FastQCFastQC Report
Fri 17 Jun 2016
SRR1528091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10512920
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA551.8189894E-1246.00000427
CGCTAAT551.8189894E-1246.00000444
ACGTAAT351.02063495E-746.00000444
AACGTTA301.8628616E-646.025
ATTAGCG950.046.031
TAACGTG253.4190693E-546.031
GTTACGT1050.046.035
TACGATT650.046.014
ATATAGC1500.046.014
TAACACG253.4190693E-546.038
CGTACTA1150.046.026
CGTACCC800.046.015
ATACGTA206.313813E-446.028
CGGTATA1050.046.023
CGACTAT206.313813E-446.034
TATACGA1000.046.031
CGATATA1000.046.024
TACGACT1350.045.99999639
CCGATTA3050.045.24590346
ACGATTC2000.044.85000225
Produced by FastQC (version 0.11.5)