FastQCFastQC Report
Fri 17 Jun 2016
SRR1528090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9288575
Sequences flagged as poor quality0
Sequence length59
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA1700.051.44117719
ATGGGAT522400.050.894819
ATTAGGA68100.050.8208541
CGGTATA1200.050.79166423
TACGGAC166750.050.4254882
ATGGGGG2159100.050.3525779
CATGGGG6281950.050.3208738
GGACATG9739650.050.279435
ACATGGG9569400.050.233537
GACATGG9716350.050.2107246
ACCGATA1900.050.2105315
ATGGGGT820650.050.1615839
TAGGACA796200.050.0710833
ATGGGAG903400.050.0666359
TACGCCT900.050.05555350
TAATCGA900.050.05555340
CGGACAT2006500.050.0482184
TGGACAT3432500.050.0199554
TCGGACA721150.050.019833
TTGGACA1292250.049.9711343
Produced by FastQC (version 0.11.5)