##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528090_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9288575
Sequences flagged as poor quality	0
Sequence length	59
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.836476208675712	31.0	31.0	33.0	28.0	34.0
2	31.423081904382535	31.0	31.0	34.0	30.0	34.0
3	31.690994474394618	31.0	31.0	34.0	30.0	34.0
4	35.44428397251462	37.0	35.0	37.0	33.0	37.0
5	35.30646595414259	37.0	35.0	37.0	33.0	37.0
6	34.984043515824546	37.0	35.0	37.0	31.0	37.0
7	34.511788622043746	35.0	35.0	37.0	30.0	37.0
8	35.18328258102023	37.0	35.0	37.0	32.0	37.0
9	36.67399272762507	38.0	35.0	39.0	32.0	39.0
10	37.00206500997193	39.0	37.0	39.0	33.0	39.0
11	36.88417276062259	39.0	37.0	39.0	33.0	39.0
12	36.8676764735172	39.0	37.0	39.0	33.0	39.0
13	36.90788683947753	39.0	37.0	39.0	33.0	39.0
14	37.30420295901148	39.0	37.0	40.0	32.0	40.0
15	36.46109893067559	38.0	36.0	40.0	30.0	40.0
16	35.886136355684265	38.0	35.0	40.0	30.0	40.0
17	36.00531545473875	38.0	35.0	40.0	30.0	40.0
18	36.24778138734951	38.0	35.0	40.0	30.0	40.0
19	36.48579970555225	39.0	35.0	40.0	30.0	40.0
20	36.51815967465408	39.0	35.0	40.0	30.0	40.0
21	36.3771492397919	39.0	35.0	40.0	30.0	40.0
22	36.37519791787223	39.0	35.0	40.0	30.0	40.0
23	36.359998062135475	39.0	35.0	40.0	30.0	40.0
24	36.32721510027103	38.0	35.0	40.0	30.0	40.0
25	36.33116080776653	38.0	35.0	40.0	30.0	40.0
26	35.99634874025348	38.0	35.0	40.0	30.0	40.0
27	35.78913041020824	38.0	35.0	40.0	29.0	40.0
28	35.719873823487454	38.0	34.0	40.0	28.0	40.0
29	35.70508016568742	38.0	34.0	40.0	28.0	40.0
30	35.600461642394016	38.0	34.0	40.0	28.0	40.0
31	35.60577515926824	38.0	34.0	40.0	28.0	40.0
32	35.546644883633924	38.0	34.0	40.0	28.0	40.0
33	35.3103650452303	38.0	34.0	40.0	27.0	40.0
34	35.18537504407296	38.0	34.0	40.0	27.0	40.0
35	35.06963339371217	38.0	33.0	40.0	26.0	40.0
36	34.8987728472882	38.0	33.0	40.0	26.0	40.0
37	34.836250770435726	37.0	33.0	40.0	26.0	40.0
38	34.711652756208565	37.0	33.0	40.0	26.0	40.0
39	34.48952772626587	37.0	33.0	40.0	25.0	40.0
40	34.40274315489728	37.0	33.0	40.0	25.0	40.0
41	34.168843660087795	36.0	33.0	39.0	24.0	40.0
42	34.12069924611687	36.0	33.0	39.0	24.0	40.0
43	33.947165415577736	36.0	33.0	39.0	24.0	40.0
44	33.721772715405756	36.0	32.0	39.0	23.0	40.0
45	33.595352462568265	35.0	32.0	39.0	23.0	40.0
46	33.44095827400866	35.0	32.0	39.0	23.0	40.0
47	33.122543231873564	35.0	32.0	39.0	23.0	40.0
48	32.924314655369635	35.0	31.0	38.0	23.0	40.0
49	32.69623596730392	35.0	31.0	38.0	22.0	40.0
50	32.361001014687396	35.0	31.0	38.0	20.0	40.0
51	32.18207884417147	35.0	31.0	37.0	20.0	40.0
52	31.54640824884334	34.0	30.0	37.0	18.0	39.0
53	31.51149255940766	34.0	30.0	37.0	19.0	39.0
54	31.533644504135456	34.0	30.0	36.0	20.0	39.0
55	31.47761351983485	34.0	30.0	36.0	20.0	39.0
56	31.29873914997726	34.0	30.0	36.0	20.0	39.0
57	31.04472408308056	34.0	29.0	36.0	18.0	39.0
58	30.8927198197786	34.0	29.0	35.0	18.0	38.0
59	30.107087470360092	33.0	28.0	35.0	16.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	11.0
13	22.0
14	97.0
15	357.0
16	1053.0
17	2918.0
18	6476.0
19	12494.0
20	21304.0
21	33404.0
22	48876.0
23	68822.0
24	93090.0
25	122124.0
26	155541.0
27	196704.0
28	240045.0
29	286333.0
30	340414.0
31	412045.0
32	498506.0
33	621098.0
34	825495.0
35	1015613.0
36	1276919.0
37	1557024.0
38	1343371.0
39	108418.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.25389255079493	22.49402088049028	32.62656543118831	16.625521137526476
2	27.08090315252878	21.916838697001424	32.72971365360134	18.272544496868466
3	20.367074605092817	22.285958825761757	35.632763906196594	21.714202662948836
4	22.703417908559707	21.77342595608045	35.03220892332785	20.490947212031983
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.4571503163833	0.4363209641952614	0.07067822566970713	0.03585049375173264
12	99.55140589379964	0.3379743394438867	0.06535986413416482	0.045259902622307516
13	98.40065887393922	0.9351918889603625	0.209235539358836	0.4549136977415804
14	65.45691884923144	23.3843512056478	4.542096069633932	6.616633875486821
15	34.238696463128086	26.41556966488401	17.350034854646704	21.995699017341195
16	27.67823912709969	19.480932220496687	26.97453592181793	25.866292730585695
17	22.786552296773184	18.688528649442997	30.391270996896726	28.13364805688709
18	23.489017421940396	19.07406679711366	30.600764918192514	26.83615086275344
19	24.315032176625586	18.797404338125062	28.891083939140287	27.996479546109065
20	27.845713685899078	19.093886844860485	24.50981985934333	28.550579609897103
21	29.478730591075596	20.950361061842102	21.311557477869318	28.259350869212984
22	30.23432550202803	21.75644810964007	21.537673970442185	26.471552417889722
23	28.461287118853	21.2206285678912	21.64448260362865	28.67360170962715
24	28.859905852081724	23.358093141305314	21.033409322743264	26.748591683869698
25	31.886559563765164	19.828972689567557	22.79189219013681	25.49257555653047
26	30.166220329813775	20.643704766339294	22.007229311277566	27.182845592569365
27	29.75205561671193	22.452216836274673	20.953676963366284	26.842050583647115
28	29.01886457287582	22.09038523131912	20.591640806043984	28.299109389761078
29	28.804439862949916	21.642157166196107	21.658790503387227	27.894612467466757
30	29.042129713115305	21.384442715917135	21.29686200520532	28.27656556576224
31	29.152641820731382	21.117814088813407	21.2557900431444	28.473754047310813
32	30.01804905488732	22.94476816949855	19.759306459817573	27.277876315796558
33	29.655797579284226	22.820658712450513	20.845920929744334	26.677622778520927
34	28.41064425921091	23.794758614749842	21.030836269287807	26.76376085675144
35	30.45932233954078	24.399566133664205	18.63983442024207	26.501277106552944
36	32.927472728594	22.731904517108383	19.866147390746157	24.47447536355146
37	32.142346915431055	23.151710569166962	20.62006281910842	24.085879696293567
38	28.357697493964356	25.879427145713958	23.3442912395066	22.418584120815087
39	30.972070527502872	24.3445092492659	22.351910815168097	22.331509408063134
40	29.860845178081675	25.273209292060407	19.87173490013269	24.994210629725227
41	31.361742786164726	25.20914133761099	18.22836118565011	25.200754690574172
42	31.756690342705955	25.669825565277776	18.398322670592638	24.17516142142363
43	31.415496994964244	24.406262532196813	19.047927157825608	25.130313315013336
44	29.01717432437161	24.132732954193727	19.911633377563298	26.93845934387137
45	27.162293462667847	24.611062514971348	21.584204250921157	26.642439771439648
46	26.896493810945167	21.782135580538455	22.777164419730692	28.544206188785687
47	27.615183168569992	20.7075251047658	24.720551860753666	26.95673986591054
48	27.707393222318817	21.050742444346955	23.15102155066843	28.090842782665803
49	28.34302355312844	21.587929257178846	23.15734114220965	26.911706047483065
50	27.788815830200004	23.557208721466964	21.64043461994978	27.013540828383253
51	27.826851804501768	23.11553709799404	23.080278729514482	25.977332367989707
52	29.156086913224044	23.04665677996894	23.364972560376593	24.43228374643043
53	27.583563679035805	23.28191353356139	23.176762851137013	25.957759936265788
54	26.847885709056555	23.75644272668305	22.31132331923896	27.084348245021438
55	26.964835833268292	24.17691626541208	22.24741685350013	26.6108310478195
56	28.37593495234737	23.297513343004713	21.47007479618779	26.856476908460124
57	27.80605205857734	22.872281270270197	21.63329681894155	27.688369852210915
58	27.93404801059366	22.72569258470756	22.696387766691878	26.643871638006907
59	27.475355477024195	22.710103541178274	22.590494236198772	27.224046745598763
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	7.5
7	14.0
8	135.5
9	938.0
10	1619.0
11	3240.5
12	4862.0
13	5932.0
14	7268.5
15	7535.0
16	8026.0
17	8517.0
18	9972.5
19	11809.0
20	12190.0
21	12995.0
22	13800.0
23	16023.0
24	18246.0
25	24308.0
26	33715.0
27	37060.0
28	44627.5
29	52195.0
30	58052.5
31	69788.5
32	75667.0
33	80723.0
34	85779.0
35	93584.5
36	99353.0
37	97316.0
38	105443.0
39	113570.0
40	121772.5
41	129975.0
42	145166.0
43	180245.0
44	200133.0
45	200529.0
46	200925.0
47	208253.5
48	249325.5
49	283069.0
50	304481.5
51	325894.0
52	376719.5
53	470166.5
54	512788.0
55	559241.5
56	605695.0
57	680051.5
58	836694.0
59	918980.0
60	910462.0
61	901944.0
62	836785.0
63	771626.0
64	697292.5
65	559330.0
66	495701.0
67	426590.5
68	357480.0
69	291248.5
70	197370.5
71	169724.0
72	151273.5
73	132823.0
74	101327.5
75	51952.0
76	34072.0
77	24000.0
78	13928.0
79	10895.5
80	7863.0
81	6388.5
82	3421.5
83	1929.0
84	1196.5
85	464.0
86	306.0
87	103.5
88	59.0
89	34.5
90	10.0
91	6.5
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	9288575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.121174472256987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.69164568322155	10.023198647243847
2	7.157033626069382	1.7350330657079411
3	2.815718313132096	1.023894388146098
4	1.6231505303899405	0.7869796309437176
5	1.057794089497589	0.6410853357261251
6	0.7040634724087781	0.5120445713165934
7	0.5002614587258344	0.4244629496073139
8	0.37152953037961994	0.36026994074616614
9	0.27323332418459423	0.29807179146685997
>10	1.43193385314084	3.3093570233526464
>50	0.1670434152371735	1.4501289680339373
>100	0.6464579405486534	23.44500959125692
>500	0.4445575246640376	37.33658846829557
>1k	0.11557723840006826	18.65387562815634
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.005813593581362049	0.0	0.0	0.0	0.0
12	0.010313745649897859	0.0	0.0	0.0	0.0
13	0.016784059987673027	0.0	0.0	0.0	0.0
14	0.04859733597457091	0.0	0.0	0.0	0.0
15	0.0826606879957367	0.0	0.0	0.0	0.0
16	0.10770220405175175	0.0	0.0	0.0	0.0
17	0.122257719833236	0.0	0.0	0.0	0.0
18	0.13874033422780135	0.0	0.0	0.0	0.0
19	0.15669787884578634	0.0	0.0	0.0	0.0
20	0.19349577303300022	0.0	0.0	0.0	0.0
21	0.23237148862984902	0.0	0.0	0.0	0.0
22	0.25544284241662474	0.0	0.0	0.0	0.0
23	0.2746492330631986	0.0	0.0	0.0	0.0
24	0.29458770586446253	0.0	0.0	0.0	0.0
25	0.3150106447975066	0.0	0.0	0.0	0.0
26	0.3393631423549899	0.0	0.0	0.0	0.0
27	0.36305891915606	0.0	0.0	0.0	0.0
28	0.38953230177933645	0.0	0.0	0.0	0.0
29	0.4210871958292849	0.0	0.0	0.0	0.0
30	0.4583264924921207	0.0	0.0	0.0	1.076591403955935E-5
31	0.4906888300950361	0.0	0.0	0.0	1.076591403955935E-5
32	0.5173559991710246	0.0	0.0	0.0	1.076591403955935E-5
33	0.540653437152631	0.0	0.0	0.0	1.076591403955935E-5
34	0.5648444459995209	0.0	0.0	0.0	1.076591403955935E-5
35	0.588249543121523	0.0	0.0	0.0	1.076591403955935E-5
36	0.6107395375501624	0.0	0.0	0.0	1.076591403955935E-5
37	0.633713998110582	0.0	0.0	0.0	1.076591403955935E-5
38	0.6551381670493052	0.0	0.0	0.0	1.076591403955935E-5
39	0.6782633504062787	0.0	0.0	0.0	1.076591403955935E-5
40	0.7012162791386192	0.0	0.0	0.0	1.076591403955935E-5
41	0.7223390024842347	0.0	0.0	0.0	1.076591403955935E-5
42	0.7430418551823073	0.0	0.0	0.0	1.076591403955935E-5
43	0.7632602417485997	0.0	0.0	0.0	1.076591403955935E-5
44	0.7845013901486504	0.0	0.0	0.0	1.076591403955935E-5
45	0.8059901545716108	0.0	0.0	0.0	1.076591403955935E-5
46	0.825605649951688	0.0	0.0	0.0	1.076591403955935E-5
47	0.845641015979308	0.0	0.0	0.0	1.076591403955935E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	170	0.0	51.441177	19
ATGGGAT	52240	0.0	50.89481	9
ATTAGGA	6810	0.0	50.820854	1
CGGTATA	120	0.0	50.791664	23
TACGGAC	16675	0.0	50.425488	2
ATGGGGG	215910	0.0	50.352577	9
CATGGGG	628195	0.0	50.320873	8
GGACATG	973965	0.0	50.27943	5
ACATGGG	956940	0.0	50.23353	7
GACATGG	971635	0.0	50.210724	6
ACCGATA	190	0.0	50.21053	15
ATGGGGT	82065	0.0	50.161583	9
TAGGACA	79620	0.0	50.071083	3
ATGGGAG	90340	0.0	50.066635	9
TACGCCT	90	0.0	50.055553	50
TAATCGA	90	0.0	50.055553	40
CGGACAT	200650	0.0	50.048218	4
TGGACAT	343250	0.0	50.019955	4
TCGGACA	72115	0.0	50.01983	3
TTGGACA	129225	0.0	49.971134	3
>>END_MODULE