FastQCFastQC Report
Fri 17 Jun 2016
SRR1528089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197151
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCGTGGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC28300.23639457344979872No Hit
AGTTCGTGGCGGGGCTTTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATC24240.20248072298314915No Hit
ACGACGTGGCGGGGAGGCAGGTTTGGGAGTGATGTAGCCACAAGCCACTGCA23880.1994735835329044No Hit
TGCGCGTGGCGGGCACCGTGTCCTCACGTGGTCAATAGGGCAAGGGAGCTCT22550.1883638738972778No Hit
TCTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22000.18376963307051492No Hit
CCAGCGTGGCGGGCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACG19980.1668962394885858No Hit
TGCACGTGGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC19720.16472441655229791No Hit
ATGGCGTGGCGGGAAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA19060.15921132756018247No Hit
TTGACGTGGCGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACTGACTGC19030.15896073260599541No Hit
GTGACGTGGCGGGAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAA18550.15495121333900236No Hit
ATTGCGTGGCGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATG18550.15495121333900236No Hit
GGCGCGTGGCGGGCGATACGACGACGAGTCGTCGGAGACACGCAGGGATGAG18440.15403236517364977No Hit
GTGACGTGGCGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCA17800.1486863394843257No Hit
ACTCCGTGGCGGGCTGCTTGAGCCCAGGAGTTCGAGACCAGCCTGGGCAATA17430.14559566838268523No Hit
AAACCGTGGCGGGGCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGA17370.1450944784743111No Hit
AGCTCGTGGCGGGACAAAACAATTCACTAAACAAGCCTACTAACAAATGTAG16770.14008257939056978No Hit
TTTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16760.1399990477391741No Hit
CATGCGTGGCGGGTAGTGGAAGACATGCATCGCCAGTGGGCAAACCTGATGG16470.13757662984869912No Hit
TGTGCGTGGCGGGGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATAT16470.13757662984869912No Hit
AAATCGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT16380.13682484498613792No Hit
CCTACGTGGCGGGGTCAAGTAATATCAATCAGGCACAAGAAATTGTGCCTGA16360.13665778168334652No Hit
TATCCGTGGCGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTG16270.13590599682078536No Hit
GTTCCGTGGCGGGGCTCTCACTTCACCAGCACCGCCCGCGCCTGCTTTCATC16090.13440242709566297No Hit
ATTGCGTGGCGGGATGCTTTGTGTACCTCAACCAAGTATGAAGAGCTGTACT15950.13323298397612332No Hit
ATATCGTGGCGGGCTTTTCCGAGTAACCGCCAAGATGGCCGAAGTGGAGCAG15840.13231413581077076No Hit
TCTGCGTGGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGC15410.12872227480075613No Hit
GGTTCGTGGCGGGGGCTGCGATTGAATACATCCGGGCAAGGAAGTCTCTCCA15200.12696811012144665No Hit
TTAGCGTGGCGGGAACGCAGAGTTTGTAAAGTTGGGAGAGTACCTCGTGGCG15090.1260492619560941No Hit
ATCGCGTGGCGGGCTCTTATCGACGGGGACTGCTCTAAGGTAATGTGTTCTC14970.12504688213934584No Hit
TTTGCGTGGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14930.12471275553376307No Hit
TTTGCGTGGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGAACCGACT14740.12312565415724498No Hit
GCGACGTGGCGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTG14370.12003498305560452No Hit
CATGCGTGGCGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACT14180.11844788167908642No Hit
TGGGCGTGGCGGGGGTGCATCGGTTTATTTCAAAGCCGCCCGTAGCGCCAGT14120.1179466917707123No Hit
TACTCGTGGCGGGTGATCTGGCATTGCTGTGAGCTGTGGTGTAGGTCACAGA14080.11761256516512955No Hit
TTGACGTGGCGGGGACACACAGTCCCTGCCATGGCGCTCAGCGAGCCTATCT14060.11744550186233817No Hit
CGAGCGTGGCGGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGA13990.11686078030256834No Hit
GCTTCGTGGCGGGGGTTGCCTTAAGTCCTCGGCTCTGTCAGTACATATTCAT13640.11393717250371925No Hit
TCCTCGTGGCGGACCTGTAGTTTTCAGAAGTGTCTGATAATTAAAACATTAT13620.11377010920092788No Hit
GCCACGTGGCGGGATCTTTGTTTCCAAACCCGCTGAATAATTTTTAGCGAGT13490.11268419773278393No Hit
TCAACGTGGCGGGGTTCAGGAAGACAAATACAATAAAAGGTCTTTCTATCAC13390.11184888121882704No Hit
AACACGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT13300.11109709635626584No Hit
TATACGTGGCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGC13290.11101356470487014No Hit
CACGCGTGGCGGGGGTACTGGTACCAAAACAGAGATATAGATCAATGGAACA13220.11042884314510033No Hit
TGTGCGTGGCGGGTGCAGAACTTGAGTATGCATGAATTTGGGTGTACTGTAG13060.1090923367227693No Hit
GCTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12510.10449809589600643No Hit
TCGACGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12500.10441456424461074No Hit
TACACGTGGCGGGGTTGTGGTGCAGGGGCTTATGCTCCACTGCACGCGGGAT12250.10232627295971852No Hit
AGAACGTGGCGGGGACTGAATTTTATTATATGTACTTAAAAGACATACCTCA12160.10157448809715733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTTA750.046.00000420
CCCCTGT750.046.00000422
CTCACAG750.046.00000440
GTTTCCA750.046.00000420
TTAGCGA750.046.00000444
TCACAGC750.046.00000441
ATGTGCC750.046.00000432
CATCTAT1750.046.00000444
TTCGTCA750.046.00000431
CACCTAT750.046.00000440
TGGTTCC750.046.00000436
ATTCCAC750.046.00000443
ACCTATT750.046.00000441
CTTATTG750.046.00000423
GCACAAC1500.046.00000417
CCTCCGT1750.046.0000041
TAATTTT750.046.00000438
TTCCAGT750.046.00000427
TATTCCA750.046.00000442
AAACATT1500.046.00000444
Produced by FastQC (version 0.11.5)