##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1528089_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1197151 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.558361476538884 31.0 31.0 31.0 30.0 34.0 2 31.30454971845657 31.0 31.0 34.0 30.0 34.0 3 31.56396561503102 31.0 31.0 34.0 30.0 34.0 4 35.50014743336472 35.0 35.0 37.0 35.0 37.0 5 35.44611247870987 35.0 35.0 37.0 35.0 37.0 6 35.34350553940146 35.0 35.0 37.0 35.0 37.0 7 33.85992911504063 35.0 35.0 35.0 31.0 37.0 8 34.73957002917761 35.0 35.0 35.0 32.0 37.0 9 36.3532394827386 37.0 35.0 39.0 32.0 39.0 10 36.64523689993994 38.0 35.0 39.0 32.0 39.0 11 37.456175536753506 39.0 37.0 39.0 35.0 39.0 12 37.38760858070536 39.0 37.0 39.0 35.0 39.0 13 36.851235140763364 39.0 37.0 39.0 34.0 39.0 14 35.087567065474616 38.0 32.0 40.0 25.0 40.0 15 32.423546403085325 36.0 30.0 39.0 21.0 40.0 16 32.622557221269496 35.0 30.0 38.0 21.0 40.0 17 32.40581012754448 35.0 30.0 38.0 21.0 40.0 18 32.567061298031746 35.0 30.0 38.0 21.0 40.0 19 32.685195100701584 35.0 30.0 39.0 22.0 40.0 20 33.13307260320544 35.0 30.0 39.0 23.0 40.0 21 33.054508579118256 35.0 30.0 38.0 23.0 40.0 22 33.62928151920685 35.0 31.0 39.0 25.0 40.0 23 33.37477895436749 35.0 31.0 38.0 24.0 40.0 24 33.29620991838122 35.0 31.0 38.0 24.0 40.0 25 33.19462958306847 35.0 31.0 38.0 24.0 40.0 26 32.76156391299009 35.0 31.0 37.0 23.0 40.0 27 32.635898896630415 35.0 31.0 37.0 22.0 40.0 28 32.633601776217034 35.0 31.0 37.0 22.0 40.0 29 32.458311441079694 35.0 31.0 37.0 22.0 40.0 30 32.22462162250209 34.0 31.0 36.0 22.0 39.0 31 31.738873375204967 34.0 30.0 36.0 21.0 39.0 32 31.215198416908144 34.0 30.0 35.0 20.0 39.0 33 30.864995309697775 34.0 29.0 35.0 18.0 39.0 34 30.6570549579794 33.0 29.0 35.0 18.0 38.0 35 30.66394882516909 33.0 29.0 35.0 18.0 37.0 36 30.681870540976035 33.0 29.0 35.0 19.0 37.0 37 30.665988668096173 33.0 30.0 35.0 20.0 36.0 38 30.175883409862248 33.0 29.0 35.0 18.0 36.0 39 30.018981732463157 33.0 28.0 35.0 17.0 36.0 40 29.814501261745594 33.0 27.0 35.0 16.0 36.0 41 29.380906836313883 33.0 26.0 35.0 15.0 35.0 42 29.02651461678602 33.0 25.0 35.0 12.0 35.0 43 28.653767152180468 33.0 25.0 35.0 10.0 35.0 44 28.275224261601085 33.0 24.0 35.0 10.0 35.0 45 28.116663645605275 33.0 23.0 35.0 9.0 35.0 46 27.80597184482158 33.0 23.0 35.0 9.0 35.0 47 27.475739484826892 33.0 23.0 35.0 9.0 35.0 48 27.112314987833614 33.0 22.0 35.0 8.0 35.0 49 26.89368926726871 33.0 20.0 34.0 8.0 35.0 50 26.50414776415005 33.0 20.0 34.0 8.0 35.0 51 26.22744248636972 32.0 20.0 34.0 8.0 35.0 52 25.57053955599586 31.0 18.0 34.0 8.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 1.0 12 8.0 13 11.0 14 47.0 15 172.0 16 428.0 17 986.0 18 1938.0 19 3764.0 20 6396.0 21 9912.0 22 14554.0 23 20265.0 24 27343.0 25 36033.0 26 45373.0 27 55795.0 28 66265.0 29 75982.0 30 85162.0 31 96177.0 32 107221.0 33 113344.0 34 124666.0 35 143251.0 36 108419.0 37 41632.0 38 11035.0 39 969.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.17965152265671 23.575889758267753 38.33685140805128 7.907607311024256 2 23.04938975952073 20.58178124564069 32.73471767554803 23.63411131929055 3 21.811200090882437 17.298569687533153 43.27758152480347 17.61264869678094 4 38.186995625447416 17.718900957356258 27.312176993545513 16.781926423650816 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 0.0 0.0 100.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 0.0 0.0 0.0 100.0 11 98.73048596208832 0.8612113258895495 0.14350737709779302 0.2647953349243328 12 97.14705997823165 1.988721556428554 0.32410280741527175 0.5401156579245224 13 93.40576084387015 4.426926929017308 1.2680939998379486 0.8992182272745878 14 46.32698799065448 33.01563461919173 8.044515687661791 12.612861702492001 15 30.130284316681855 37.00009439076607 21.585915227068263 11.283706065483804 16 36.56982285442689 29.64212534592545 18.79604160210366 14.992010197544003 17 23.288123219209606 29.543223870672957 25.455435446322145 21.713217463795296 18 19.718982818374624 34.71525313013981 29.111782891214222 16.453981160271343 19 22.129706277654197 36.618688870493365 28.4283269194947 12.82327793235774 20 19.279773395336093 32.792354515011056 30.61100897046404 17.31686311918881 21 24.587374525018145 32.40543590574623 20.786851449817107 22.22033811941852 22 27.789894507877456 33.976081546939355 19.864829081711495 18.36919486347169 23 25.840516359256267 34.811398060896245 20.78351018376128 18.56457539608621 24 21.917619414760544 35.176347845844006 20.49866725250198 22.407365486893465 25 28.582108689714165 33.81144066203846 19.702861209655257 17.903589438592125 26 27.442235774768598 34.348382117209944 19.607718658715566 18.601663449305896 27 27.764417354201765 39.242000382574965 14.492825048803368 18.500757214419902 28 27.5777241133324 37.99996825797247 15.304669168718066 19.117638459977062 29 25.566866669283993 39.295210044514015 13.919714388577548 21.218208897624443 30 27.937327872590846 42.11807867177992 14.701570645641194 15.243022809988046 31 25.684228639494933 44.452454201683835 13.296067079257337 16.567250079563898 32 22.518546114901127 49.50453200974647 13.994475216576689 13.98244665877571 33 23.190641782030838 49.38115576063504 13.057250087917064 14.37095236941706 34 21.010883338860346 53.14408959270802 13.825741280757398 12.019285787674235 35 20.864034695706724 51.43920858772202 15.5926863027304 12.10407041384086 36 20.89953564754989 50.42705556776046 15.90050043812351 12.772908346566139 37 20.84490594753711 51.56684495105463 15.310098726058785 12.278150375349476 38 18.286582060241358 52.02727141354766 17.804771494991027 11.881375031219955 39 19.04680361959352 54.52411600541619 13.766266744963668 12.662813630026621 40 21.15622841228884 53.535184784542636 12.058879790435793 13.249707012732731 41 21.458780053644027 51.42500820698474 12.517635619900917 14.598576119470309 42 21.317611562785313 52.604140998086294 13.39187788340819 12.686369555720207 43 20.560814801140374 52.31562267416558 15.123990206749191 11.999572317944853 44 21.46103540823171 51.3445672266907 13.38511181964514 13.809285545432449 45 22.018525649646538 50.26124523974001 14.458911198336718 13.261317912276729 46 19.897740552361398 50.61959602422752 12.384152040970605 17.098511382440478 47 21.452849306394935 49.89646251809504 14.85276293466739 13.797925240842634 48 22.106066820309216 50.33057651039844 13.809202013781052 13.754154655511295 49 21.153889526049763 51.2845915009886 13.48935932058696 14.07215965237468 50 18.51395521534042 53.08461505691429 14.673838137377823 13.727591590367464 51 18.467762212118604 51.67627141438298 15.518927854548007 14.337038518950408 52 21.761832884907584 49.645449905651 14.98783361497422 13.604883594467198 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 2.5 10 5.0 11 43.0 12 81.0 13 704.5 14 2072.5 15 2817.0 16 7536.5 17 12256.0 18 16164.0 19 20072.0 20 19046.5 21 18021.0 22 14740.5 23 11460.0 24 12901.5 25 14343.0 26 16727.0 27 19111.0 28 23558.5 29 28006.0 30 34363.5 31 40721.0 32 47098.0 33 53475.0 34 53274.0 35 53073.0 36 49987.0 37 46901.0 38 44880.5 39 42281.0 40 41702.0 41 45948.5 42 50195.0 43 48455.5 44 46716.0 45 46924.0 46 47132.0 47 46594.5 48 46057.0 49 46618.5 50 47180.0 51 46356.0 52 45532.0 53 50000.5 54 54469.0 55 55687.0 56 56905.0 57 60779.5 58 64654.0 59 64124.0 60 63594.0 61 61169.0 62 58744.0 63 63945.5 64 59148.0 65 49149.0 66 43004.0 67 36859.0 68 30222.0 69 23585.0 70 18276.5 71 12968.0 72 9577.0 73 6186.0 74 6469.0 75 6752.0 76 4193.5 77 1635.0 78 1229.0 79 823.0 80 1063.5 81 1304.0 82 910.5 83 517.0 84 464.0 85 411.0 86 292.5 87 174.0 88 141.0 89 85.5 90 63.0 91 51.0 92 39.0 93 25.5 94 12.0 95 10.5 96 9.0 97 4.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1197151.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.239109477214484 #Duplication Level Percentage of deduplicated Percentage of total 1 84.59335676347244 29.809945600330686 2 6.491627475989345 4.575183426233642 3 2.2714766958188086 2.4013444787670126 4 1.2246784162061077 1.7262630713227474 5 0.7738028491250528 1.363406165704911 6 0.547478516438169 1.1575593226312566 7 0.4497645662512548 1.1094511952369899 8 0.3613778546289071 1.0187707025527168 9 0.2950632761001065 0.9357990380277497 >10 2.515545630202944 18.031248522818906 >50 0.23648163005430736 5.7047524039360065 >100 0.18655636764925387 14.026275830824918 >500 0.03415820815861996 8.72281965929744 >1k 0.018631749904701796 9.417180582315037 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGCCGTGGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC 2830 0.23639457344979872 No Hit AGTTCGTGGCGGGGCTTTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATC 2424 0.20248072298314915 No Hit ACGACGTGGCGGGGAGGCAGGTTTGGGAGTGATGTAGCCACAAGCCACTGCA 2388 0.1994735835329044 No Hit TGCGCGTGGCGGGCACCGTGTCCTCACGTGGTCAATAGGGCAAGGGAGCTCT 2255 0.1883638738972778 No Hit TCTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2200 0.18376963307051492 No Hit CCAGCGTGGCGGGCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACG 1998 0.1668962394885858 No Hit TGCACGTGGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC 1972 0.16472441655229791 No Hit ATGGCGTGGCGGGAAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA 1906 0.15921132756018247 No Hit TTGACGTGGCGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACTGACTGC 1903 0.15896073260599541 No Hit GTGACGTGGCGGGAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAA 1855 0.15495121333900236 No Hit ATTGCGTGGCGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATG 1855 0.15495121333900236 No Hit GGCGCGTGGCGGGCGATACGACGACGAGTCGTCGGAGACACGCAGGGATGAG 1844 0.15403236517364977 No Hit GTGACGTGGCGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCA 1780 0.1486863394843257 No Hit ACTCCGTGGCGGGCTGCTTGAGCCCAGGAGTTCGAGACCAGCCTGGGCAATA 1743 0.14559566838268523 No Hit AAACCGTGGCGGGGCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGA 1737 0.1450944784743111 No Hit AGCTCGTGGCGGGACAAAACAATTCACTAAACAAGCCTACTAACAAATGTAG 1677 0.14008257939056978 No Hit TTTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1676 0.1399990477391741 No Hit CATGCGTGGCGGGTAGTGGAAGACATGCATCGCCAGTGGGCAAACCTGATGG 1647 0.13757662984869912 No Hit TGTGCGTGGCGGGGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATAT 1647 0.13757662984869912 No Hit AAATCGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 1638 0.13682484498613792 No Hit CCTACGTGGCGGGGTCAAGTAATATCAATCAGGCACAAGAAATTGTGCCTGA 1636 0.13665778168334652 No Hit TATCCGTGGCGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTG 1627 0.13590599682078536 No Hit GTTCCGTGGCGGGGCTCTCACTTCACCAGCACCGCCCGCGCCTGCTTTCATC 1609 0.13440242709566297 No Hit ATTGCGTGGCGGGATGCTTTGTGTACCTCAACCAAGTATGAAGAGCTGTACT 1595 0.13323298397612332 No Hit ATATCGTGGCGGGCTTTTCCGAGTAACCGCCAAGATGGCCGAAGTGGAGCAG 1584 0.13231413581077076 No Hit TCTGCGTGGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGC 1541 0.12872227480075613 No Hit GGTTCGTGGCGGGGGCTGCGATTGAATACATCCGGGCAAGGAAGTCTCTCCA 1520 0.12696811012144665 No Hit TTAGCGTGGCGGGAACGCAGAGTTTGTAAAGTTGGGAGAGTACCTCGTGGCG 1509 0.1260492619560941 No Hit ATCGCGTGGCGGGCTCTTATCGACGGGGACTGCTCTAAGGTAATGTGTTCTC 1497 0.12504688213934584 No Hit TTTGCGTGGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1493 0.12471275553376307 No Hit TTTGCGTGGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGAACCGACT 1474 0.12312565415724498 No Hit GCGACGTGGCGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTG 1437 0.12003498305560452 No Hit CATGCGTGGCGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACT 1418 0.11844788167908642 No Hit TGGGCGTGGCGGGGGTGCATCGGTTTATTTCAAAGCCGCCCGTAGCGCCAGT 1412 0.1179466917707123 No Hit TACTCGTGGCGGGTGATCTGGCATTGCTGTGAGCTGTGGTGTAGGTCACAGA 1408 0.11761256516512955 No Hit TTGACGTGGCGGGGACACACAGTCCCTGCCATGGCGCTCAGCGAGCCTATCT 1406 0.11744550186233817 No Hit CGAGCGTGGCGGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGA 1399 0.11686078030256834 No Hit GCTTCGTGGCGGGGGTTGCCTTAAGTCCTCGGCTCTGTCAGTACATATTCAT 1364 0.11393717250371925 No Hit TCCTCGTGGCGGACCTGTAGTTTTCAGAAGTGTCTGATAATTAAAACATTAT 1362 0.11377010920092788 No Hit GCCACGTGGCGGGATCTTTGTTTCCAAACCCGCTGAATAATTTTTAGCGAGT 1349 0.11268419773278393 No Hit TCAACGTGGCGGGGTTCAGGAAGACAAATACAATAAAAGGTCTTTCTATCAC 1339 0.11184888121882704 No Hit AACACGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 1330 0.11109709635626584 No Hit TATACGTGGCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGC 1329 0.11101356470487014 No Hit CACGCGTGGCGGGGGTACTGGTACCAAAACAGAGATATAGATCAATGGAACA 1322 0.11042884314510033 No Hit TGTGCGTGGCGGGTGCAGAACTTGAGTATGCATGAATTTGGGTGTACTGTAG 1306 0.1090923367227693 No Hit GCTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1251 0.10449809589600643 No Hit TCGACGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1250 0.10441456424461074 No Hit TACACGTGGCGGGGTTGTGGTGCAGGGGCTTATGCTCCACTGCACGCGGGAT 1225 0.10232627295971852 No Hit AGAACGTGGCGGGGACTGAATTTTATTATATGTACTTAAAAGACATACCTCA 1216 0.10157448809715733 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.031240837621987535 0.0 0.0 0.0 0.0 12 0.11176534956743134 0.0 0.0 0.0 0.0 13 0.12613279360748977 0.0 0.0 0.0 0.0 14 0.28267110832301023 0.0 0.0 0.0 0.0 15 0.444471917076459 0.0 0.0 0.0 0.0 16 0.5854733446323814 0.0 0.0 0.0 0.0 17 0.6836230350223155 0.0 0.0 0.0 0.0 18 0.8193619685403094 0.0 0.0 0.0 0.0 19 0.9532632057275983 0.0 0.0 0.0 0.0 20 1.1028683933772765 0.0 0.0 0.0 0.0 21 1.2472946186404221 0.0 0.0 0.0 0.0 22 1.400742262254302 0.0 0.0 0.0 0.0 23 1.5281280306327272 0.0 0.0 0.0 0.0 24 1.672721319198664 0.0 0.0 0.0 0.0 25 1.8173146077646012 0.0 0.0 0.0 0.0 26 1.9841273156017913 0.0 0.0 0.0 0.0 27 2.1681475436264934 0.0 0.0 0.0 0.0 28 2.340473340455799 0.0 0.0 0.0 0.0 29 2.5741949010609355 0.0 0.0 0.0 0.0 30 2.869061630487716 0.0 0.0 0.0 0.0 31 3.099274861734234 0.0 0.0 0.0 0.0 32 3.262412176910014 0.0 0.0 0.0 0.0 33 3.390883856756583 0.0 0.0 0.0 0.0 34 3.538651348075556 0.0 0.0 0.0 0.0 35 3.6778150793007733 0.0 0.0 0.0 0.0 36 3.8239954692432283 0.0 0.0 0.0 0.0 37 3.9768583912973385 0.0 0.0 0.0 0.0 38 4.132310794544715 0.0 0.0 0.0 0.0 39 4.292190375316063 0.0 0.0 0.0 0.0 40 4.458752488199066 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCTTA 75 0.0 46.000004 20 CCCCTGT 75 0.0 46.000004 22 CTCACAG 75 0.0 46.000004 40 GTTTCCA 75 0.0 46.000004 20 TTAGCGA 75 0.0 46.000004 44 TCACAGC 75 0.0 46.000004 41 ATGTGCC 75 0.0 46.000004 32 CATCTAT 175 0.0 46.000004 44 TTCGTCA 75 0.0 46.000004 31 CACCTAT 75 0.0 46.000004 40 TGGTTCC 75 0.0 46.000004 36 ATTCCAC 75 0.0 46.000004 43 ACCTATT 75 0.0 46.000004 41 CTTATTG 75 0.0 46.000004 23 GCACAAC 150 0.0 46.000004 17 CCTCCGT 175 0.0 46.000004 1 TAATTTT 75 0.0 46.000004 38 TTCCAGT 75 0.0 46.000004 27 TATTCCA 75 0.0 46.000004 42 AAACATT 150 0.0 46.000004 44 >>END_MODULE