FastQCFastQC Report
Fri 17 Jun 2016
SRR1528088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202621
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCGTGGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC28370.23590141865142883No Hit
AGTTCGTGGCGGGGCTTTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATC23810.1979842360976567No Hit
TGCGCGTGGCGGGCACCGTGTCCTCACGTGGTCAATAGGGCAAGGGAGCTCT22520.18725766471731328No Hit
ACGACGTGGCGGGGAGGCAGGTTTGGGAGTGATGTAGCCACAAGCCACTGCA21460.17844358280788378No Hit
TCTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20870.1735376315564089No Hit
CCAGCGTGGCGGGCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACG19900.16547191509211964No Hit
ATGGCGTGGCGGGAAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA19400.16131432928578496No Hit
TGCACGTGGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC19260.16015020526001125No Hit
TTGACGTGGCGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACTGACTGC18420.15316546110536902No Hit
GGCGCGTGGCGGGCGATACGACGACGAGTCGTCGGAGACACGCAGGGATGAG18350.15258339909248217No Hit
ATTGCGTGGCGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATG18310.1522507922279754No Hit
GTGACGTGGCGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCA18230.15158557849896184No Hit
GTGACGTGGCGGGAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAA18190.15125297163445509No Hit
ACTCCGTGGCGGGCTGCTTGAGCCCAGGAGTTCGAGACCAGCCTGGGCAATA17570.1460975652346001No Hit
AAACCGTGGCGGGGCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGA17400.1446839860604463No Hit
TATCCGTGGCGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTG17180.14285464830565905No Hit
TGTGCGTGGCGGGGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATAT17130.14243888972502558No Hit
TTTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16720.13902966936383115No Hit
GTTCCGTGGCGGGGCTCTCACTTCACCAGCACCGCCCGCGCCTGCTTTCATC16340.13586990415101682No Hit
CATGCGTGGCGGGTAGTGGAAGACATGCATCGCCAGTGGGCAAACCTGATGG16280.13537099385425666No Hit
ATTGCGTGGCGGGATGCTTTGTGTACCTCAACCAAGTATGAAGAGCTGTACT16210.1347889318413698No Hit
CCTACGTGGCGGGGTCAAGTAATATCAATCAGGCACAAGAAATTGTGCCTGA15970.13279329065432915No Hit
AAATCGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT15930.1324606837898224No Hit
GCGACGTGGCGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTG15100.12555909135130686No Hit
AGCTCGTGGCGGGACAAAACAATTCACTAAACAAGCCTACTAACAAATGTAG15000.12472757419003991No Hit
TCTGCGTGGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGC14970.12447811904165984No Hit
ATATCGTGGCGGGCTTTTCCGAGTAACCGCCAAGATGGCCGAAGTGGAGCAG14920.12406236046102637No Hit
ATCGCGTGGCGGGCTCTTATCGACGGGGACTGCTCTAAGGTAATGTGTTCTC14610.12148465726109889No Hit
TTAGCGTGGCGGGAACGCAGAGTTTGTAAAGTTGGGAGAGTACCTCGTGGCG14600.1214015055449722No Hit
CGAGCGTGGCGGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGA14370.11948901607405824No Hit
CATGCGTGGCGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACT14340.11923956092567817No Hit
TGGGCGTGGCGGGGGTGCATCGGTTTATTTCAAAGCCGCCCGTAGCGCCAGT14330.11915640920955148No Hit
TTTGCGTGGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGAACCGACT13980.11624609914511722No Hit
GCTTCGTGGCGGGGGTTGCCTTAAGTCCTCGGCTCTGTCAGTACATATTCAT13960.11607979571286381No Hit
TTTGCGTGGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13760.11441676139032994No Hit
GCCACGTGGCGGGATCTTTGTTTCCAAACCCGCTGAATAATTTTTAGCGAGT13570.11283687878392279No Hit
TATACGTGGCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGC13560.11275372706779609No Hit
GGTTCGTGGCGGGGGCTGCGATTGAATACATCCGGGCAAGGAAGTCTCTCCA13470.11200536162265584No Hit
TCAACGTGGCGGGGTTCAGGAAGACAAATACAATAAAAGGTCTTTCTATCAC13390.1113401478936423No Hit
TCCTCGTGGCGGACCTGTAGTTTTCAGAAGTGTCTGATAATTAAAACATTAT13200.10976026528723512No Hit
CACGCGTGGCGGGGGTACTGGTACCAAAACAGAGATATAGATCAATGGAACA13110.10901189984209489No Hit
TTGACGTGGCGGGGACACACAGTCCCTGCCATGGCGCTCAGCGAGCCTATCT13070.10867929297758812No Hit
TACTCGTGGCGGGTGATCTGGCATTGCTGTGAGCTGTGGTGTAGGTCACAGA12980.10793092753244787No Hit
TACACGTGGCGGGGTTGTGGTGCAGGGGCTTATGCTCCACTGCACGCGGGAT12610.10485431403576023No Hit
TGTGCGTGGCGGGTGCAGAACTTGAGTATGCATGAATTTGGGTGTACTGTAG12480.10377334172611322No Hit
AACACGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT12030.10003151450041202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAT1450.046.00000438
TCTTGTA1050.046.00000430
TCCATGC1150.046.00000424
ACGCCTT1450.046.00000426
TCATTTC1150.046.00000423
TCCGAGA1050.046.00000427
TAGGTCA1450.046.00000442
ACATTGT1450.046.00000446
CCCAGCG1550.046.00000429
ATGCTCG1150.046.00000427
CTAAGGT1450.046.00000435
CTGACAT1150.046.00000417
TGTGCCT1150.046.00000444
TGTGACT1050.046.00000427
TGTTGGC1050.046.00000424
TAACCTT1050.046.00000435
GTTAAGG1150.046.00000414
TATCTGA1150.046.00000437
TTCCAGT1150.046.00000427
CATAGGG1050.046.00000432
Produced by FastQC (version 0.11.5)