##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528088_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1202621
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.585975964164938	31.0	31.0	31.0	30.0	34.0
2	31.331293898909134	31.0	31.0	34.0	30.0	34.0
3	31.593314103113116	31.0	31.0	34.0	30.0	34.0
4	35.53588952795602	35.0	35.0	37.0	35.0	37.0
5	35.55375550568301	35.0	35.0	37.0	35.0	37.0
6	35.39006553186748	35.0	35.0	37.0	35.0	37.0
7	33.845496627782154	35.0	35.0	35.0	31.0	37.0
8	34.730437103626166	35.0	35.0	35.0	32.0	37.0
9	36.35037139714008	37.0	35.0	39.0	32.0	39.0
10	36.62136034544549	38.0	35.0	39.0	32.0	39.0
11	37.46786560354426	39.0	37.0	39.0	35.0	39.0
12	37.41138978946817	39.0	37.0	39.0	35.0	39.0
13	36.86930213259206	39.0	37.0	39.0	34.0	39.0
14	35.07377885468489	38.0	32.0	40.0	25.0	40.0
15	32.459784088253905	36.0	30.0	39.0	21.0	40.0
16	32.62115412918949	35.0	30.0	38.0	21.0	40.0
17	32.43177276964231	35.0	30.0	38.0	21.0	40.0
18	32.524851137640205	35.0	30.0	38.0	21.0	40.0
19	32.702103156355996	35.0	30.0	39.0	22.0	40.0
20	33.16449904001344	35.0	30.0	39.0	23.0	40.0
21	33.13128824459243	35.0	30.0	38.0	23.0	40.0
22	33.62181851140135	35.0	31.0	39.0	24.0	40.0
23	33.519319885483455	35.0	31.0	38.0	25.0	40.0
24	33.53792341893248	35.0	31.0	38.0	26.0	40.0
25	33.36681797507278	35.0	31.0	38.0	24.0	40.0
26	32.79663751090327	35.0	31.0	38.0	22.0	40.0
27	32.642937384263206	35.0	31.0	37.0	22.0	40.0
28	32.68029828183609	35.0	31.0	37.0	22.0	40.0
29	32.49063753252271	35.0	31.0	37.0	22.0	40.0
30	32.11393115536815	34.0	31.0	36.0	21.0	39.0
31	31.696999304020135	34.0	30.0	36.0	21.0	39.0
32	31.28372197059589	34.0	30.0	35.0	20.0	39.0
33	31.016584609781468	34.0	29.0	35.0	18.0	39.0
34	30.869205676601357	33.0	29.0	35.0	18.0	38.0
35	30.730713998840866	33.0	29.0	35.0	18.0	37.0
36	30.762166135465787	33.0	30.0	35.0	20.0	37.0
37	30.750364412395925	33.0	30.0	35.0	20.0	37.0
38	30.317303622670817	33.0	29.0	35.0	18.0	36.0
39	30.16666846828718	33.0	29.0	35.0	18.0	36.0
40	29.900505645585767	33.0	27.0	35.0	17.0	36.0
41	29.44342980872611	33.0	26.0	35.0	15.0	35.0
42	29.103263621706258	33.0	25.0	35.0	12.0	35.0
43	28.77476362045898	33.0	25.0	35.0	10.0	35.0
44	28.334637429414588	33.0	24.0	35.0	10.0	35.0
45	28.170646446386684	33.0	23.0	35.0	10.0	35.0
46	27.854214253700874	33.0	23.0	35.0	9.0	35.0
47	27.632140133924153	33.0	23.0	35.0	9.0	35.0
48	27.272164713571442	33.0	23.0	35.0	8.0	35.0
49	26.896978349787673	33.0	20.0	34.0	8.0	35.0
50	26.510058447341265	33.0	20.0	34.0	8.0	35.0
51	26.258161964575706	32.0	20.0	34.0	8.0	35.0
52	25.425239539306233	31.0	17.0	34.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	5.0
13	14.0
14	50.0
15	143.0
16	412.0
17	890.0
18	1921.0
19	3696.0
20	6248.0
21	9828.0
22	14555.0
23	20161.0
24	26890.0
25	35438.0
26	44902.0
27	55547.0
28	65542.0
29	75288.0
30	84656.0
31	95488.0
32	106844.0
33	115097.0
34	127339.0
35	146348.0
36	110941.0
37	42492.0
38	11004.0
39	879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.327010754011447	23.508570031622597	38.20314130553184	7.961277908834122
2	22.970079517986132	20.571485114595536	32.772835332162	23.68560003525633
3	21.902910393216153	17.240261063127953	43.22949624195819	17.62733230169771
4	38.073507780090324	17.56147614252537	27.616763718578007	16.748252358806308
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	0.0	100.0
11	98.7564660853253	0.8494779319502985	0.13420686982848296	0.2598491128959165
12	97.22106964704591	1.9459164607968764	0.3143966386750273	0.518617253482186
13	93.45745667171951	4.365548248367524	1.2745495047899547	0.9024455751230022
14	46.43457914006158	32.92375569693195	8.075029456495438	12.566635706511029
15	30.240283514091303	36.82382063842225	21.618448372346734	11.317447475139716
16	36.61868535473769	29.462814968306724	18.96682329678261	14.951676380172973
17	23.401803228115924	29.449344390294197	25.431869225633015	21.716983155956864
18	19.774642218953435	34.4842639534816	29.33891891127795	16.40217491628701
19	22.132409129725826	36.42095057378842	28.598619182601997	12.848021113883759
20	19.21253661793699	32.623162243133955	30.7287166946195	17.435584444309555
21	24.642177377577806	32.26552671207305	20.78468611474438	22.307609795604765
22	27.753465139890288	33.885737900801665	19.998486638766494	18.36231032054155
23	25.8573565570533	34.71983276526853	20.797824085892398	18.624986591785774
24	22.22811675498765	34.90301599589563	20.39686651072948	22.47200073838724
25	28.551472159558166	33.65806850204678	19.795596451417364	17.994862886977693
26	27.43931795636364	34.130952311659286	19.731902236864315	18.697827495112758
27	27.83412230453318	39.045135583030735	14.567265996519268	18.553476115916816
28	27.642956509157912	37.884587081050476	15.26324586049969	19.20921054929192
29	25.59418137551232	39.14591546297628	13.92375486541479	21.33614829609661
30	27.93024568837564	42.025625695875924	14.846489459272705	15.19763915647573
31	25.658457652078255	44.48267575570358	13.250891178517588	16.607975413700576
32	22.472083890103367	49.67691400698973	13.94562376675611	13.90537833615079
33	23.292708176557703	49.43327947873853	13.01523921501454	14.258773129689237
34	20.883886112083523	53.29351474820413	13.75778404002591	12.064815099686435
35	20.719744624449433	51.500431141648114	15.643664961779313	12.136159272123137
36	20.778699191183257	50.54651465424269	15.930372078984151	12.7444140755899
37	20.744606987571313	51.69949635005542	15.261416522744906	12.294480139628362
38	18.197420467462315	52.14227923843006	17.762703295551965	11.897596998555654
39	19.095126394766098	54.615294427754044	13.691345818840684	12.598233358639172
40	21.134588536205502	53.604751621666345	12.012595821958872	13.24806402016928
41	21.39044636672734	51.542672213440476	12.5198212903317	14.547060129500483
42	21.33714611669013	52.69798215730476	13.34693141064392	12.617940315361198
43	20.623870695755354	52.36570789966248	15.053204625563666	11.957216779018493
44	21.358599259450816	51.41977397700522	13.412371811235626	13.809254952308333
45	21.88652950513919	50.47541993695437	14.403872874330318	13.234177683576123
46	19.824450096913328	50.763540633333356	12.360253147084578	17.05175612266874
47	21.41863479849429	50.06440100414012	14.72816456722442	13.788799630141165
48	22.141389515067505	50.508930078553426	13.66806333832521	13.681617068053859
49	20.930367921398346	51.46226450394597	13.571357892469862	14.036009682185826
50	18.35449405922564	53.31654777357122	14.58788762211869	13.741070545084444
51	18.28838844490492	51.84933574251572	15.628614501160381	14.233661311418977
52	21.87946160926842	49.538466399638786	15.000652740971596	13.581419250121193
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	3.0
11	38.0
12	73.0
13	656.5
14	2020.0
15	2800.0
16	7425.0
17	12050.0
18	15787.0
19	19524.0
20	18647.0
21	17770.0
22	14520.0
23	11270.0
24	12725.0
25	14180.0
26	16785.0
27	19390.0
28	23884.5
29	28379.0
30	34649.5
31	40920.0
32	47385.5
33	53851.0
34	53659.0
35	53467.0
36	50503.0
37	47539.0
38	45496.5
39	43067.5
40	42681.0
41	46918.5
42	51156.0
43	48954.5
44	46753.0
45	47079.5
46	47406.0
47	46978.0
48	46550.0
49	46669.0
50	46788.0
51	46351.5
52	45915.0
53	50557.0
54	55199.0
55	56491.0
56	57783.0
57	60923.0
58	64063.0
59	63509.0
60	62955.0
61	61088.0
62	59221.0
63	64456.5
64	59322.0
65	48952.0
66	43130.0
67	37308.0
68	30500.5
69	23693.0
70	18281.5
71	12870.0
72	9471.0
73	6072.0
74	6386.0
75	6700.0
76	4129.5
77	1559.0
78	1172.0
79	785.0
80	1035.5
81	1286.0
82	931.0
83	576.0
84	481.5
85	387.0
86	275.0
87	163.0
88	120.0
89	70.5
90	64.0
91	50.5
92	37.0
93	27.0
94	17.0
95	9.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1202621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.66728888867213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.15409250649776	30.372156174819665
2	6.210334578200917	4.430115949920038
3	2.172583223789024	2.324704604326972
4	1.1677277632267975	1.6659873389733248
5	0.743716560266826	1.326317670316321
6	0.5464572781050374	1.1694389762093895
7	0.41587144957479993	1.0383104992774663
8	0.34810283383031	0.9932707469752879
9	0.27715167461328116	0.8896723959970043
>10	2.488854927881835	18.033520965976912
>50	0.23451210287113836	5.691295530431508
>100	0.18746758367769897	13.99440280226807
>500	0.03393607943772463	8.520687626469968
>1k	0.019191438026851172	9.550118718038037
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGCCGTGGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC	2837	0.23590141865142883	No Hit
AGTTCGTGGCGGGGCTTTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATC	2381	0.1979842360976567	No Hit
TGCGCGTGGCGGGCACCGTGTCCTCACGTGGTCAATAGGGCAAGGGAGCTCT	2252	0.18725766471731328	No Hit
ACGACGTGGCGGGGAGGCAGGTTTGGGAGTGATGTAGCCACAAGCCACTGCA	2146	0.17844358280788378	No Hit
TCTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2087	0.1735376315564089	No Hit
CCAGCGTGGCGGGCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACG	1990	0.16547191509211964	No Hit
ATGGCGTGGCGGGAAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA	1940	0.16131432928578496	No Hit
TGCACGTGGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC	1926	0.16015020526001125	No Hit
TTGACGTGGCGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	1842	0.15316546110536902	No Hit
GGCGCGTGGCGGGCGATACGACGACGAGTCGTCGGAGACACGCAGGGATGAG	1835	0.15258339909248217	No Hit
ATTGCGTGGCGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATG	1831	0.1522507922279754	No Hit
GTGACGTGGCGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCA	1823	0.15158557849896184	No Hit
GTGACGTGGCGGGAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAA	1819	0.15125297163445509	No Hit
ACTCCGTGGCGGGCTGCTTGAGCCCAGGAGTTCGAGACCAGCCTGGGCAATA	1757	0.1460975652346001	No Hit
AAACCGTGGCGGGGCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGA	1740	0.1446839860604463	No Hit
TATCCGTGGCGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTG	1718	0.14285464830565905	No Hit
TGTGCGTGGCGGGGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATAT	1713	0.14243888972502558	No Hit
TTTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1672	0.13902966936383115	No Hit
GTTCCGTGGCGGGGCTCTCACTTCACCAGCACCGCCCGCGCCTGCTTTCATC	1634	0.13586990415101682	No Hit
CATGCGTGGCGGGTAGTGGAAGACATGCATCGCCAGTGGGCAAACCTGATGG	1628	0.13537099385425666	No Hit
ATTGCGTGGCGGGATGCTTTGTGTACCTCAACCAAGTATGAAGAGCTGTACT	1621	0.1347889318413698	No Hit
CCTACGTGGCGGGGTCAAGTAATATCAATCAGGCACAAGAAATTGTGCCTGA	1597	0.13279329065432915	No Hit
AAATCGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1593	0.1324606837898224	No Hit
GCGACGTGGCGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTG	1510	0.12555909135130686	No Hit
AGCTCGTGGCGGGACAAAACAATTCACTAAACAAGCCTACTAACAAATGTAG	1500	0.12472757419003991	No Hit
TCTGCGTGGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGC	1497	0.12447811904165984	No Hit
ATATCGTGGCGGGCTTTTCCGAGTAACCGCCAAGATGGCCGAAGTGGAGCAG	1492	0.12406236046102637	No Hit
ATCGCGTGGCGGGCTCTTATCGACGGGGACTGCTCTAAGGTAATGTGTTCTC	1461	0.12148465726109889	No Hit
TTAGCGTGGCGGGAACGCAGAGTTTGTAAAGTTGGGAGAGTACCTCGTGGCG	1460	0.1214015055449722	No Hit
CGAGCGTGGCGGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGA	1437	0.11948901607405824	No Hit
CATGCGTGGCGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACT	1434	0.11923956092567817	No Hit
TGGGCGTGGCGGGGGTGCATCGGTTTATTTCAAAGCCGCCCGTAGCGCCAGT	1433	0.11915640920955148	No Hit
TTTGCGTGGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGAACCGACT	1398	0.11624609914511722	No Hit
GCTTCGTGGCGGGGGTTGCCTTAAGTCCTCGGCTCTGTCAGTACATATTCAT	1396	0.11607979571286381	No Hit
TTTGCGTGGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1376	0.11441676139032994	No Hit
GCCACGTGGCGGGATCTTTGTTTCCAAACCCGCTGAATAATTTTTAGCGAGT	1357	0.11283687878392279	No Hit
TATACGTGGCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGC	1356	0.11275372706779609	No Hit
GGTTCGTGGCGGGGGCTGCGATTGAATACATCCGGGCAAGGAAGTCTCTCCA	1347	0.11200536162265584	No Hit
TCAACGTGGCGGGGTTCAGGAAGACAAATACAATAAAAGGTCTTTCTATCAC	1339	0.1113401478936423	No Hit
TCCTCGTGGCGGACCTGTAGTTTTCAGAAGTGTCTGATAATTAAAACATTAT	1320	0.10976026528723512	No Hit
CACGCGTGGCGGGGGTACTGGTACCAAAACAGAGATATAGATCAATGGAACA	1311	0.10901189984209489	No Hit
TTGACGTGGCGGGGACACACAGTCCCTGCCATGGCGCTCAGCGAGCCTATCT	1307	0.10867929297758812	No Hit
TACTCGTGGCGGGTGATCTGGCATTGCTGTGAGCTGTGGTGTAGGTCACAGA	1298	0.10793092753244787	No Hit
TACACGTGGCGGGGTTGTGGTGCAGGGGCTTATGCTCCACTGCACGCGGGAT	1261	0.10485431403576023	No Hit
TGTGCGTGGCGGGTGCAGAACTTGAGTATGCATGAATTTGGGTGTACTGTAG	1248	0.10377334172611322	No Hit
AACACGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1203	0.10003151450041202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.02918625236046934	0.0	0.0	0.0	0.0
12	0.10626789320991402	0.0	0.0	0.0	0.0
13	0.12181726412560566	0.0	0.0	0.0	0.0
14	0.28030443506308306	0.0	0.0	0.0	0.0
15	0.4402883368908409	0.0	0.0	0.0	0.0
16	0.5769897582031247	0.0	0.0	0.0	0.0
17	0.6770212727035367	0.0	0.0	0.0	0.0
18	0.804908612106391	0.0	0.0	0.0	0.0
19	0.9367040821671998	0.0	0.0	0.0	0.0
20	1.0769810272729314	0.0	0.0	0.0	0.0
21	1.21858839983669	0.0	0.0	0.0	0.0
22	1.370423433484032	0.0	0.0	0.0	0.0
23	1.5042976964480081	0.0	0.0	0.0	0.0
24	1.6446577932698665	0.0	0.0	0.0	0.0
25	1.788427110452919	0.0	0.0	0.0	0.0
26	1.9495751363064506	0.0	0.0	0.0	0.0
27	2.13442140125609	0.0	0.0	0.0	0.0
28	2.3166899630058015	0.0	0.0	0.0	0.0
29	2.538871348496326	0.0	0.0	0.0	0.0
30	2.8257447691334177	0.0	0.0	0.0	0.0
31	3.0573222985462585	0.0	0.0	0.0	0.0
32	3.2240414893802787	0.0	0.0	0.0	0.0
33	3.362489096731223	0.0	0.0	0.0	0.0
34	3.5124947926237775	0.0	0.0	0.0	0.0
35	3.6577608406971107	0.0	0.0	0.0	0.0
36	3.807932840021919	0.0	0.0	0.0	0.0
37	3.959435266804754	0.0	0.0	0.0	0.0
38	4.107195866361888	0.0	0.0	0.0	0.0
39	4.27009007825408	0.0	0.0	0.0	0.0
40	4.428660400907684	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAAT	145	0.0	46.000004	38
TCTTGTA	105	0.0	46.000004	30
TCCATGC	115	0.0	46.000004	24
ACGCCTT	145	0.0	46.000004	26
TCATTTC	115	0.0	46.000004	23
TCCGAGA	105	0.0	46.000004	27
TAGGTCA	145	0.0	46.000004	42
ACATTGT	145	0.0	46.000004	46
CCCAGCG	155	0.0	46.000004	29
ATGCTCG	115	0.0	46.000004	27
CTAAGGT	145	0.0	46.000004	35
CTGACAT	115	0.0	46.000004	17
TGTGCCT	115	0.0	46.000004	44
TGTGACT	105	0.0	46.000004	27
TGTTGGC	105	0.0	46.000004	24
TAACCTT	105	0.0	46.000004	35
GTTAAGG	115	0.0	46.000004	14
TATCTGA	115	0.0	46.000004	37
TTCCAGT	115	0.0	46.000004	27
CATAGGG	105	0.0	46.000004	32
>>END_MODULE