SRR1528087_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528087_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	679760
Sequences flagged as poor quality	0
Sequence length	59
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGCGCGTGGCGGGCACCGTGTCCTCACGTGGTCAATAGGGCAAGGGAGCTCTCACGACC	1576	0.23184653407084854	No Hit
TCTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1527	0.2246381075673767	No Hit
CCTACGTGGCGGGGTCAAGTAATATCAATCAGGCACAAGAAATTGTGCCTGATTTTTTA	1402	0.20624926444627514	No Hit
ATTGCGTGGCGGGATGCTTTGTGTACCTCAACCAAGTATGAAGAGCTGTACTGGTTTTA	1395	0.2052194892314935	No Hit
ATTGCGTGGCGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCAC	1327	0.1952159585736142	No Hit
ACTCCGTGGCGGGCTGCTTGAGCCCAGGAGTTCGAGACCAGCCTGGGCAATATGGTGAA	1298	0.19094974696951866	No Hit
TGCACGTGGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCA	1266	0.18624220313051665	No Hit
GTGACGTGGCGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA	1255	0.18462398493585971	No Hit
TTTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1206	0.1774155584323879	No Hit
TCAACGTGGCGGGGTTCAGGAAGACAAATACAATAAAAGGTCTTTCTATCACGCCTTAC	1143	0.16814758149935272	No Hit
GCCACGTGGCGGGATCTTTGTTTCCAAACCCGCTGAATAATTTTTAGCGAGTATACCTG	1067	0.15696716488172296	No Hit
TTTGCGTGGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1053	0.1549076144521596	No Hit
CATGCGTGGCGGGTAGTGGAAGACATGCATCGCCAGTGGGCAAACCTGATGGAGAAGAT	1053	0.1549076144521596	No Hit
TTTGCGTGGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGAACCGACTGCTCTTC	1049	0.15431917147228433	No Hit
TATACGTGGCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG	1049	0.15431917147228433	No Hit
TCTGCGTGGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCT	1041	0.15314228551253384	No Hit
AACACGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	1004	0.14769918794868775	No Hit
TCCTCGTGGCGGACCTGTAGTTTTCAGAAGTGTCTGATAATTAAAACATTATCTAAAGA	980	0.14416853006943628	No Hit
ATCGCGTGGCGGGCTCTTATCGACGGGGACTGCTCTAAGGTAATGTGTTCTCTGAGGAA	973	0.14313875485465458	No Hit
GTTACGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	936	0.13769565729080852	No Hit
CCAGCGTGGCGGGCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACGCCAGAGC	930	0.13681299282099565	No Hit
CCCACGTGGCGGGAAAGACGTGAGCAATCACATAAAATTAAGACTGAAGCCAACTTCCT	919	0.13519477462633872	No Hit
CATGCGTGGCGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTC	918	0.1350476638813699	No Hit
AATACGTGGCGGGGGAGAGATGGCTCAGCGGTTAAGAGAACTGACTGCTCTTCCGAAGG	909	0.13372366717665057	No Hit
AACACGTGGCGGGGTACTGACATAAGGACAGACATACAGATCAATGGAAAAGAGTCCAG	882	0.12975167706249263	No Hit
TTGACGTGGCGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCG	881	0.12960456631752382	No Hit
GCTGCGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	870	0.12798634812286688	No Hit
TATCCGTGGCGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCC	838	0.1232788042838649	No Hit
AAACCGTGGCGGGGCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTGT	837	0.12313169353889608	No Hit
CACGCGTGGCGGGGGTACTGGTACCAAAACAGAGATATAGATCAATGGAACAGAACAGA	830	0.12210191832411439	No Hit
ATGCCGTGGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCA	823	0.12107214310933272	No Hit
GTTCCGTGGCGGGGCTCTCACTTCACCAGCACCGCCCGCGCCTGCTTTCATCGTGGGTC	798	0.11739437448511239	No Hit
ATGCCGTGGCGGGGATAAAGTAAAAGAAACACAATAAAGCATGGAAATTTTATGAAAGA	785	0.11548193480051784	No Hit
AGCTCGTGGCGGGACAAAACAATTCACTAAACAAGCCTACTAACAAATGTAGCTACCTA	767	0.1128339413910792	No Hit
ATTACGTGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTT	764	0.11239260915617277	No Hit
TCACCGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	762	0.11209838766623513	No Hit
CGTCCGTGGCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	736	0.10827350829704602	No Hit
AGCGCGTGGCGGGGGGCCCAGAGCAGCTCCTTTCCCAGGCTGGGTCAGGCGCCCACCCA	729	0.10724373308226433	No Hit
GGGTCGTGGCGGAGATCAGGGCTCCCTGGTACCATGGACACAGATGGATAACAGTCCCA	729	0.10724373308226433	No Hit
TTCTCGTGGCCGGGTTCTCAGATGAATACACATGGGCTTGTTTTGCTAAACCTTATTGC	727	0.1069495115923267	No Hit
TCTGCGTGGCGGTCTGGGCGCACTGGCTTCCTGTGCCCACGGCTCCACGCAGCTCAGCT	725	0.10665529010238908	No Hit
TGTACGTGGCGGGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAAGGT	716	0.10533129339766976	No Hit
TGTCCGTGGCGGGAACCCCATCTCTATTAAAAGTACAAAAATTAGCTGGGCATAGTGGC	708	0.10415440743791926	No Hit
AGAACGTGGCGGGGACTGAATTTTATTATATGTACTTAAAAGACATACCTCAGTGTGGC	706	0.10386018594798165	No Hit
CAACCGTGGCGGGACCAAATGGATATACATACAATGGGATATTACACAGCCATAAAAAG	700	0.10297752147816876	No Hit
CGTGCGTGGCGGGGTATATATAGACAAACCATTCAATAAAAGGAGGAAACCTATCATGA	695	0.10224196775332471	No Hit
CACGCGTGGCGGGATATTTTCATGAAGGCAGAGGAAAAGAGTACTCAGATAAAGAGTAC	693	0.10194774626338708	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTTA	45	7.6397555E-11	53.000004	20
CTTAAGA	65	0.0	53.000004	25
TGTCAGG	45	7.6397555E-11	53.000004	28
TCTTGTA	45	7.6397555E-11	53.000004	30
TGATAAT	150	0.0	53.000004	35
TCGTTCC	75	0.0	53.000004	18
TCGCACC	75	0.0	53.000004	42
CTGTACT	215	0.0	53.000004	46
ACGCACC	45	7.6397555E-11	53.000004	53
TCACAGT	45	7.6397555E-11	53.000004	53
TGGACAC	90	0.0	53.000004	35
ATAATTA	155	0.0	53.000004	37
ATGTGTG	65	0.0	53.000004	41
ATAATCT	90	0.0	53.000004	45
AACTTGT	45	7.6397555E-11	53.000004	34
GACGTGA	90	0.0	53.000004	17
ATTACAC	65	0.0	53.000004	41
ACATTGT	75	0.0	53.000004	46
CGCTGTA	90	0.0	53.000004	21
TTATCGA	150	0.0	53.000004	17

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content