FastQCFastQC Report
Fri 17 Jun 2016
SRR1528086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10829815
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT750.046.00000420
CACGATT700.046.00000418
CATACGT700.046.00000418
TTTACGC405.6188583E-946.015
GCTATAC501.6370905E-1146.034
CGATTAT206.3138205E-446.026
TACGCAC301.8628671E-646.014
CGCTATA253.4190765E-546.035
CGAATAT206.3138205E-446.024
CATAACG650.046.036
TATAGGT453.110472E-1046.017
TGTCGAT253.4190765E-546.033
ACCTTAT551.8189894E-1246.044
ACCGAAT1050.043.80952541
CGTAACA1050.043.80952517
TATAGCG1000.043.69999725
CGACTAA950.043.57894531
CTTACGT1900.043.57894538
CCCACGA1800.043.44444734
TACGGGT800.043.12520
Produced by FastQC (version 0.11.5)