##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10829815
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93852027943229	31.0	31.0	33.0	30.0	34.0
2	31.64897027326875	31.0	31.0	34.0	30.0	34.0
3	31.98246396637431	33.0	31.0	34.0	30.0	34.0
4	35.63526357560124	37.0	35.0	37.0	33.0	37.0
5	35.55822200102218	37.0	35.0	37.0	33.0	37.0
6	35.22163425691021	37.0	35.0	37.0	32.0	37.0
7	35.500558412124306	37.0	35.0	37.0	33.0	37.0
8	34.9302301101173	37.0	35.0	37.0	32.0	37.0
9	36.995593091848754	39.0	37.0	39.0	33.0	39.0
10	37.21956386143253	39.0	37.0	39.0	33.0	39.0
11	37.46719791612322	39.0	37.0	39.0	35.0	39.0
12	37.36217987103196	39.0	37.0	39.0	35.0	39.0
13	37.29397750561759	39.0	37.0	39.0	35.0	39.0
14	37.81416109139445	40.0	38.0	40.0	33.0	40.0
15	37.17597392014545	39.0	37.0	40.0	31.0	40.0
16	36.87247880042272	39.0	36.0	40.0	31.0	40.0
17	36.77389715336781	39.0	36.0	40.0	31.0	40.0
18	36.9095208920928	39.0	36.0	40.0	31.0	40.0
19	36.95765310857111	39.0	36.0	40.0	31.0	40.0
20	36.94576749464326	39.0	36.0	40.0	31.0	40.0
21	36.899194584579696	39.0	36.0	40.0	31.0	40.0
22	36.9097956890307	39.0	36.0	40.0	31.0	40.0
23	36.80396082481557	39.0	35.0	40.0	31.0	40.0
24	36.849117274856496	39.0	35.0	40.0	31.0	40.0
25	36.97329492701399	39.0	36.0	40.0	31.0	40.0
26	36.85228214886404	39.0	35.0	40.0	31.0	40.0
27	36.662199769802164	39.0	35.0	40.0	31.0	40.0
28	36.56902791044907	39.0	35.0	40.0	30.0	40.0
29	36.591738732379085	39.0	35.0	40.0	31.0	40.0
30	36.52009143277147	39.0	35.0	40.0	30.0	40.0
31	36.52017721447689	39.0	35.0	40.0	31.0	40.0
32	36.452050104272324	39.0	35.0	40.0	31.0	40.0
33	36.30993761204601	39.0	35.0	40.0	30.0	40.0
34	36.11125720984153	38.0	35.0	40.0	30.0	40.0
35	36.04034944271901	38.0	35.0	40.0	30.0	40.0
36	35.83296953826081	38.0	35.0	40.0	30.0	40.0
37	35.65871393001635	38.0	35.0	40.0	29.0	40.0
38	35.39664380231795	38.0	34.0	40.0	28.0	40.0
39	35.17680856044171	37.0	34.0	40.0	28.0	40.0
40	34.85847172827975	37.0	34.0	40.0	26.0	40.0
41	34.764351099257006	37.0	33.0	40.0	27.0	40.0
42	34.53927772542744	36.0	33.0	40.0	26.0	40.0
43	34.30180090795641	36.0	33.0	39.0	26.0	40.0
44	34.024815105336515	35.0	33.0	39.0	24.0	40.0
45	33.86988438860682	35.0	33.0	39.0	24.0	40.0
46	33.624945116791004	35.0	33.0	39.0	23.0	40.0
47	33.270761596573905	35.0	33.0	39.0	23.0	40.0
48	33.01526978992716	35.0	33.0	38.0	22.0	40.0
49	32.87899091535728	35.0	32.0	38.0	22.0	40.0
50	32.67188100627758	35.0	32.0	37.0	21.0	40.0
51	32.42178753746024	35.0	32.0	37.0	20.0	40.0
52	31.565608923144115	34.0	31.0	36.0	18.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	4.0
13	15.0
14	35.0
15	97.0
16	301.0
17	807.0
18	2072.0
19	4430.0
20	9064.0
21	16569.0
22	27092.0
23	41820.0
24	61717.0
25	87947.0
26	119610.0
27	156715.0
28	203818.0
29	257588.0
30	318615.0
31	385115.0
32	460312.0
33	570162.0
34	775878.0
35	1124845.0
36	1359717.0
37	2018058.0
38	2388319.0
39	439090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.294129216427056	20.231241253890303	35.37627374059483	15.098355789087812
2	19.295112612726996	23.57863915496248	35.85427821250871	21.271970019801817
3	21.640305028294573	23.652841715209355	36.77904931894035	17.92780393755572
4	24.397646681868526	22.147811389206556	35.52396786094684	17.93057406797808
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	0.0	100.0
11	99.53769293381282	0.324585415355664	0.09385201870946087	0.043869632122063025
12	99.16644928837658	0.6147104082572048	0.144914756161578	0.0739255472046383
13	94.09270610809142	4.242584014593047	0.6222266954698672	1.0424831818456732
14	50.52791760524072	32.18128841536074	6.785776118982642	10.505017860415897
15	33.25860137038352	28.129704893389224	18.86335085133033	19.748342884896925
16	31.206377948284437	18.725213680935454	26.5237771836361	23.54463118714401
17	24.602562462978362	17.902060192163948	28.007043518287244	29.488333826570447
18	23.4376764515368	22.749612989695578	28.249014410680147	25.563696148087477
19	27.718155850307692	19.372473121655357	29.597633939268587	23.311737088768368
20	27.72549669592694	20.333366728794537	26.368243594188822	25.572892981089705
21	34.68789633063907	20.6419684916132	19.47918777929263	25.190947398455098
22	32.78938744567659	21.709502886245055	21.302616896041158	24.198492772037195
23	28.547856080644035	24.461461253031562	22.133619087676013	24.85706357864839
24	28.199124361773492	25.790283582868224	21.472019605136374	24.53857245022191
25	30.781606149320183	21.641967106547987	23.360399046521106	24.216027697610716
26	29.27615106998596	21.75625345400637	19.612569559129124	29.355025916878542
27	29.02015408388786	25.78035728218811	18.752093179800394	26.44739545412364
28	31.619367459185593	24.982116499681666	18.59265370645759	24.805862334675155
29	29.512563233997994	22.102085769701514	19.938022948683795	28.447328047616693
30	30.97366852527028	22.01790150616608	18.981496913843866	28.02693305471977
31	27.49072814263217	22.16506930173784	20.770530244514795	29.573672311115196
32	28.5277172324735	26.272895705051287	19.278316388599436	25.92107067387578
33	30.665546918391495	24.55614431086773	18.406223929032954	26.37208484170782
34	28.58833691988275	26.149033940099624	19.97568748865978	25.286941651357846
35	33.842369421822994	24.120652107168958	18.624297829648985	23.412680641359064
36	30.203313722348906	23.580799856691918	21.18602210656415	25.029864314395027
37	30.33530120320615	24.617668907548282	23.094845110465876	21.952184778779692
38	28.170056459874893	26.43793084184725	24.308753196615086	21.08325950166277
39	30.831071444895414	26.760383256777704	21.33057674577082	21.07796855255607
40	30.25272361531568	28.562907122605512	18.554453607933283	22.629915654145524
41	31.667909377953364	29.70053505069108	16.706259525208882	21.92529604614668
42	30.576228679806626	30.183996679537	17.46506288426903	21.774711756387344
43	31.292639809636636	28.393208932931913	17.546883303177385	22.767267954254066
44	28.272689792023225	28.874546795120693	19.238066393562587	23.614697019293494
45	26.74138016208033	26.931466511662478	21.486276543043438	24.840876783213748
46	26.602984446179367	26.59037111898957	18.851171511240036	27.955472923591028
47	29.323040144268393	24.893435391093938	21.212938540501383	24.570585924136285
48	29.840916026728063	24.62386476592629	20.614294888693852	24.920924318651796
49	29.0610227413857	25.395078309278595	20.461605299813524	25.082293649522175
50	27.583379771491938	26.983378755777455	20.466831612543704	24.9664098601869
51	26.671397433843513	26.459334716243998	21.839348132909013	25.029919717003473
52	29.266603353796906	25.481838794106825	21.137138538377616	24.114419313718656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	3.0
6	6.0
7	245.0
8	484.0
9	828.0
10	1172.0
11	2600.5
12	4029.0
13	10112.0
14	20892.5
15	25590.0
16	24994.0
17	24398.0
18	24279.0
19	24160.0
20	26111.5
21	28063.0
22	32700.5
23	37338.0
24	47367.0
25	57396.0
26	70173.0
27	82950.0
28	94328.0
29	105706.0
30	107201.0
31	108696.0
32	105765.5
33	102835.0
34	103441.0
35	104047.0
36	109347.5
37	114648.0
38	125014.5
39	156433.0
40	177485.0
41	182497.5
42	187510.0
43	216212.0
44	244914.0
45	290070.5
46	335227.0
47	367681.0
48	400135.0
49	453312.5
50	506490.0
51	548709.5
52	590929.0
53	694587.0
54	798245.0
55	859228.5
56	920212.0
57	946549.0
58	972886.0
59	976994.0
60	981102.0
61	965230.5
62	949359.0
63	917893.5
64	772266.0
65	658104.0
66	548550.5
67	438997.0
68	369522.0
69	300047.0
70	254312.5
71	208578.0
72	180961.5
73	153345.0
74	119183.5
75	85022.0
76	62424.0
77	39826.0
78	27300.0
79	14774.0
80	9733.5
81	4693.0
82	2575.5
83	458.0
84	861.5
85	1265.0
86	793.5
87	322.0
88	344.0
89	183.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.0829815E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.519163590189702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2656585162754	10.048589096395549
2	8.100095030900976	2.0281282957586404
3	2.9847112706388006	1.1209826599983035
4	1.6693303322790374	0.83594478063468
5	1.0808402176081155	0.6765607749546116
6	0.7831061847694323	0.5882300661370707
7	0.5979213915347992	0.5239833000288614
8	0.4565250182217407	0.4572249108905846
9	0.35235296046368353	0.397004791817925
>10	2.423279657655631	6.123723302222276
>50	0.2743875786060104	2.3947835344765616
>100	0.4975784246723053	17.18986008033348
>500	0.37467850337054365	33.398932587464536
>1k	0.13945811888538834	24.135563567432914
>5k	7.679411832895832E-5	0.08048825145403275
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.3634581938841983	0.0	0.0	0.0	0.0
7	1.3634581938841983	0.0	0.0	0.0	0.0
8	1.3634581938841983	0.0	0.0	0.0	0.0
9	1.3634581938841983	0.0	0.0	0.0	0.0
10	1.3634581938841983	0.0	0.0	0.0	0.0
11	1.3761730925228177	0.0	0.0	0.0	0.0
12	1.4018245002338452	0.0	0.0	0.0	0.0
13	1.4336902338590272	0.0	0.0	0.0	0.0
14	1.538595072953693	0.0	0.0	0.0	0.0
15	1.642124080605255	0.0	0.0	0.0	0.0
16	1.681672309268441	0.0	0.0	9.233768074523895E-6	0.0
17	1.7339539041063952	0.0	0.0	9.233768074523895E-6	0.0
18	1.8358023659683937	0.0	0.0	9.233768074523895E-6	0.0
19	1.8964312871457176	0.0	0.0	9.233768074523895E-6	0.0
20	1.9661185348041494	0.0	0.0	9.233768074523895E-6	0.0
21	2.030302456690165	0.0	0.0	9.233768074523895E-6	0.0
22	2.089592481496683	0.0	0.0	9.233768074523895E-6	0.0
23	2.1674331463649192	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
24	2.2457447334049565	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
25	2.3268356846354252	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
26	2.3889604762408223	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
27	2.4476687736586453	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
28	2.5222129833242763	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
29	2.5937931534379857	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
30	2.681061495510311	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
31	2.7661691358531977	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
32	2.8898554592114456	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
33	2.956052342537707	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
34	3.0442809964897832	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
35	3.0984924488553127	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
36	3.168521345932502	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
37	3.2257430066903265	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
38	3.282059758176848	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
39	3.34146058820026	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
40	3.4088855626804335	0.0	0.0	9.233768074523895E-6	6.463637652166726E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAT	75	0.0	46.000004	20
CACGATT	70	0.0	46.000004	18
CATACGT	70	0.0	46.000004	18
TTTACGC	40	5.6188583E-9	46.0	15
GCTATAC	50	1.6370905E-11	46.0	34
CGATTAT	20	6.3138205E-4	46.0	26
TACGCAC	30	1.8628671E-6	46.0	14
CGCTATA	25	3.4190765E-5	46.0	35
CGAATAT	20	6.3138205E-4	46.0	24
CATAACG	65	0.0	46.0	36
TATAGGT	45	3.110472E-10	46.0	17
TGTCGAT	25	3.4190765E-5	46.0	33
ACCTTAT	55	1.8189894E-12	46.0	44
ACCGAAT	105	0.0	43.809525	41
CGTAACA	105	0.0	43.809525	17
TATAGCG	100	0.0	43.699997	25
CGACTAA	95	0.0	43.578945	31
CTTACGT	190	0.0	43.578945	38
CCCACGA	180	0.0	43.444447	34
TACGGGT	80	0.0	43.125	20
>>END_MODULE