FastQCFastQC Report
Fri 17 Jun 2016
SRR1528085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10778949
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT351.02063495E-746.00000444
CGACGTA700.046.00000435
CGTAACC1450.046.00000439
AACGTAC1250.046.014
TAATAGG551.8189894E-1246.016
CCGATAA253.419075E-546.023
ACCGAAC253.419075E-546.044
TACGGTA1100.046.029
CATTACG301.8628652E-646.018
CGTTACG1000.046.020
ACGATTA650.046.026
TATAGCC453.110472E-1046.031
CTTACGT2550.044.1960838
CGTAACA1250.044.1617
ACAAACG1100.043.90909244
CATACGT800.043.12518
ATACGTA800.043.12514
TACCTAG750.042.93333441
GCGACTA2800.042.71428719
ACCGAAT650.042.4615441
Produced by FastQC (version 0.11.5)