FastQCFastQC Report
Fri 17 Jun 2016
SRR1528084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9558971
Sequences flagged as poor quality0
Sequence length59
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAG457.6397555E-1153.053
GCGACTA2350.049.6170219
TCGGGGG1785850.048.500859
TCGGGAT555250.048.489879
ATCGGGG5077900.048.483228
CTTACGT1700.048.32353238
CGGGATT148000.047.95067610
ATCGGGC1016150.047.935498
CGGGGGC481900.047.73189510
CGGGGGG730350.047.709810
CACGAGA122900.047.3938182
TATAGAG75150.047.3932151
AGAGATC2385300.047.361824
TCGGGGC1299100.047.3271149
TAGAGAT864000.047.310483
GATCGGG10008150.047.2242627
TCGGGGA1343250.047.1880539
CTGAGAT831950.047.1358873
TACGGTA900.047.1111129
TGAGATC3808500.047.1064724
Produced by FastQC (version 0.11.5)