##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528084_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9558971
Sequences flagged as poor quality	0
Sequence length	59
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.473040665151093	31.0	31.0	31.0	28.0	33.0
2	31.192034895806252	31.0	31.0	33.0	30.0	34.0
3	31.615765755540004	31.0	31.0	34.0	30.0	34.0
4	35.381109640357735	37.0	35.0	37.0	33.0	37.0
5	35.26585173236743	37.0	35.0	37.0	33.0	37.0
6	34.78520167076561	35.0	35.0	37.0	32.0	37.0
7	35.01227642598769	36.0	35.0	37.0	32.0	37.0
8	34.376906991348754	35.0	35.0	37.0	30.0	37.0
9	36.537289317019585	38.0	35.0	39.0	32.0	39.0
10	36.83050497799397	39.0	35.0	39.0	33.0	39.0
11	36.71211095838663	37.0	35.0	39.0	33.0	39.0
12	36.72943970642865	39.0	37.0	39.0	32.0	39.0
13	36.74073276297208	39.0	37.0	39.0	32.0	39.0
14	36.97938962258595	39.0	36.0	40.0	31.0	40.0
15	36.32860629036326	38.0	36.0	40.0	30.0	40.0
16	35.80179048560771	38.0	35.0	40.0	30.0	40.0
17	35.91804149212295	38.0	35.0	40.0	30.0	40.0
18	36.14401236283696	38.0	35.0	40.0	30.0	40.0
19	36.31889949242444	38.0	35.0	40.0	30.0	40.0
20	36.20019100382248	38.0	35.0	40.0	30.0	40.0
21	36.13625012566729	38.0	35.0	40.0	30.0	40.0
22	36.04164109295865	38.0	35.0	40.0	30.0	40.0
23	35.90722934508327	38.0	34.0	40.0	29.0	40.0
24	35.97967751968282	38.0	34.0	40.0	30.0	40.0
25	36.01228845657132	38.0	35.0	40.0	30.0	40.0
26	35.68863259444976	38.0	34.0	40.0	28.0	40.0
27	35.46629537844607	38.0	34.0	40.0	27.0	40.0
28	35.36046620499215	38.0	34.0	40.0	27.0	40.0
29	35.265143497140016	38.0	34.0	40.0	27.0	40.0
30	35.11204825289249	38.0	34.0	40.0	26.0	40.0
31	35.139673715926115	38.0	34.0	40.0	26.0	40.0
32	35.06705491626661	38.0	33.0	40.0	26.0	40.0
33	34.78665329144737	37.0	33.0	40.0	26.0	40.0
34	34.55092666354987	37.0	33.0	40.0	25.0	40.0
35	34.426871992811776	37.0	33.0	40.0	24.0	40.0
36	34.06322678455662	37.0	33.0	40.0	24.0	40.0
37	33.97652854057199	36.0	33.0	39.0	23.0	40.0
38	33.835843627938615	36.0	32.0	39.0	23.0	40.0
39	33.511393328842615	36.0	32.0	39.0	23.0	40.0
40	33.20277287168253	35.0	31.0	39.0	22.0	40.0
41	33.0582461229352	35.0	31.0	39.0	22.0	40.0
42	32.94895527980993	35.0	31.0	39.0	22.0	40.0
43	32.76657278278174	35.0	31.0	38.0	21.0	40.0
44	32.496688712623985	35.0	31.0	38.0	20.0	40.0
45	32.37279451941009	35.0	31.0	38.0	20.0	40.0
46	32.14497449568578	35.0	31.0	38.0	20.0	40.0
47	31.756706658070204	35.0	30.0	37.0	18.0	40.0
48	31.475041612742626	34.0	30.0	37.0	18.0	40.0
49	31.212305906148266	34.0	29.0	37.0	15.0	40.0
50	30.79448729366372	34.0	29.0	36.0	14.0	39.0
51	30.52924085657337	34.0	28.0	36.0	13.0	39.0
52	29.942280398172564	34.0	27.0	36.0	12.0	39.0
53	29.910590690148553	33.0	27.0	35.0	12.0	39.0
54	29.879167538012197	33.0	27.0	35.0	12.0	38.0
55	29.787947468404287	33.0	27.0	35.0	12.0	38.0
56	29.501859248239167	33.0	27.0	35.0	11.0	38.0
57	29.240570977775747	33.0	26.0	35.0	10.0	37.0
58	29.082144197319984	33.0	26.0	35.0	10.0	37.0
59	28.285200153865933	33.0	25.0	35.0	9.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	8.0
13	23.0
14	94.0
15	429.0
16	1490.0
17	3882.0
18	8728.0
19	17184.0
20	30343.0
21	48252.0
22	71342.0
23	100041.0
24	133741.0
25	171617.0
26	215871.0
27	265624.0
28	313677.0
29	362512.0
30	420427.0
31	491969.0
32	577115.0
33	696656.0
34	889591.0
35	1091531.0
36	1318298.0
37	1378615.0
38	896989.0
39	52919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.58161500856107	20.600376337578595	34.50347322949301	15.314535424367328
2	18.743513292382623	24.087278850411828	35.68120459827737	21.488003258928185
3	21.60574605781313	23.85382275979287	36.66031626207465	17.88011492031935
4	24.480731241887856	22.296604937916435	35.51600899301818	17.70665482717753
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	0.0	100.0
11	99.48383565553239	0.36280055667079647	0.09969692344500261	0.053666864351821976
12	99.16943989054889	0.6145954412875612	0.14134366554726446	0.07462100261628579
13	94.1809426977025	4.162759778222991	0.6329865421707002	1.0233109819038053
14	50.29757910134888	32.42811386288336	6.833130888251465	10.441176147516297
15	33.27379066219575	28.455677917633604	18.711083023476064	19.55944839669458
16	31.673199971001065	18.59272300334419	26.384325258440473	23.349751767214276
17	24.48903757527876	17.96736280505506	27.79509426276113	29.748505356905046
18	23.234007091349056	23.233588636266393	28.024731950750763	25.507672321633784
19	28.036365002048864	19.452438970680003	29.406041717251785	23.10515431001935
20	27.70153816765424	20.42986635276956	26.671500520296586	25.197094959279614
21	34.70450951258247	20.728747895563234	19.58709781628169	24.979644775572602
22	33.01059287657636	21.812285025239643	21.225537769703454	23.951584328480543
23	28.401163681739384	24.57254028702462	22.47558863814944	24.550707393086558
24	28.052768441289338	26.40523755119667	21.26584545554119	24.276148551972803
25	30.557745179894365	21.937403094956558	23.56808070659488	23.936771018554193
26	28.977522789848404	22.094532978497373	19.74588059739903	29.182063634255194
27	28.846337121432843	26.281521306006685	18.909472578167673	25.962668994392807
28	31.36586563553755	25.472971933903764	18.527161553267607	24.634000877291083
29	29.35787753723701	22.321607629105685	19.65583952498653	28.664675308670777
30	31.028622223040536	22.347478614591466	18.900287489103167	27.72361167326483
31	27.10896392509194	22.617518140812436	20.64532887483391	29.62818905926171
32	28.253281655525477	27.09816778395917	18.990161179482605	25.658389381032748
33	30.73229325625112	25.112859951139093	18.334494371831443	25.82035242077835
34	28.381642752133047	26.875528757227112	19.790592522981814	24.952235967658027
35	33.692821120599696	24.47270736567775	18.664916966481016	23.16955454724154
36	30.002664512738868	23.96691024588316	21.487490651451918	24.54293458992605
37	30.17356156849937	25.196184819474816	22.951277914746264	21.67897569727955
38	27.989728183085816	27.02154865832316	24.12274291866771	20.865980239923314
39	30.292863112567243	27.5024372393221	21.386350057971722	20.818349590138936
40	30.02617122700759	29.15781416221474	18.424859746933013	22.391154863844655
41	31.66705914266295	30.233986482436237	16.523776460876384	21.57517791402443
42	30.28853210246166	31.0287268368112	17.26701545595232	21.415725604774824
43	31.25475534971285	28.87208257039382	17.359849716041612	22.513312363851714
44	27.981369542809574	29.550178570475836	19.007882752233478	23.46056913448111
45	26.372545747863445	27.310000208181407	21.588610322177985	24.728843721777167
46	26.27025440290592	26.951352818206058	18.59240916203219	28.185983616855832
47	29.376551095300947	25.161348433842935	21.012313982331364	24.449786488524758
48	29.92678814487459	24.846408677251976	20.448435297062833	24.7783678808106
49	29.083444232648052	25.623678531925663	20.360350502161793	24.93252673326449
50	27.157044414090176	27.636478863676857	20.477653923209935	24.728822799023035
51	26.35216698533765	26.980058836876896	21.812745325830573	24.85502885195488
52	28.928166012848035	25.908343063285788	21.295932375984822	23.867558547881355
53	25.812014703256242	26.29860473475649	20.999697561589002	26.88968300039826
54	25.912266079685775	27.95551948007793	21.478954167765547	24.65326027247075
55	28.110253708270484	25.93256115119504	21.337411736054015	24.619773404480462
56	26.874493080897516	25.767679387247856	23.187297042746547	24.170530489108085
57	27.258593001275973	24.782636122653788	22.36098425238449	25.59778662368575
58	28.432872115628342	24.950656299720965	21.079496945853272	25.536974638797417
59	25.397189718433083	25.51743278643695	21.76560636076833	27.319771134361638
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	3.0
5	6.0
6	158.5
7	311.0
8	559.0
9	1802.0
10	2797.0
11	7227.5
12	11658.0
13	14900.0
14	17776.5
15	17411.0
16	16677.5
17	15944.0
18	15383.0
19	15741.5
20	16661.0
21	19111.5
22	21562.0
23	26704.5
24	31847.0
25	39483.0
26	55531.0
27	63943.0
28	73444.0
29	82945.0
30	88698.5
31	101021.5
32	107591.0
33	109026.5
34	110462.0
35	115504.0
36	116209.0
37	111872.0
38	123484.5
39	135097.0
40	136812.5
41	138528.0
42	158899.0
43	199747.0
44	220224.0
45	227263.5
46	234303.0
47	257494.5
48	305179.0
49	329672.0
50	387019.0
51	444366.0
52	484065.0
53	578896.5
54	634029.0
55	673512.5
56	712996.0
57	737763.5
58	777745.0
59	792959.0
60	782634.5
61	772310.0
62	713311.0
63	654312.0
64	603539.5
65	481301.0
66	409835.0
67	344344.5
68	278854.0
69	245443.0
70	190681.5
71	169331.0
72	139923.0
73	110515.0
74	85330.5
75	46242.0
76	32338.0
77	24161.5
78	15985.0
79	11105.0
80	6225.0
81	4306.0
82	1666.5
83	946.0
84	837.5
85	729.0
86	630.0
87	429.5
88	328.0
89	202.5
90	77.0
91	38.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	9558971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.639950146579036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.2265600749449	17.797119012578648
2	5.9699023393275406	2.46436973327332
3	2.3259988659017643	1.4402550189963537
4	1.2565672625163615	1.0374194261664396
5	0.7554590522414284	0.7796318588022465
6	0.5203114188460274	0.6443521047406684
7	0.3603300565811252	0.52060360829039
8	0.28140437000999663	0.4646537734428649
9	0.2158173111875529	0.4009012689311843
>10	1.2894072157303842	5.231288558072955
>50	0.13764812797768847	2.0094882711435047
>100	0.3998762933964023	23.814275025453732
>500	0.21279360516961482	30.071872205154733
>1k	0.04786892110450221	13.246752939655332
>5k	5.508506456214293E-5	0.07701719529763304
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.232611752875911	0.0	0.0	0.0	0.0
7	1.232611752875911	0.0	0.0	0.0	0.0
8	1.232611752875911	0.0	0.0	0.0	0.0
9	1.232611752875911	0.0	0.0	0.0	0.0
10	1.232611752875911	0.0	0.0	0.0	0.0
11	1.2443494179446721	0.0	0.0	0.0	0.0
12	1.2672389109664628	0.0	0.0	0.0	0.0
13	1.2950975580949038	0.0	0.0	0.0	0.0
14	1.3876807451345965	0.0	0.0	0.0	0.0
15	1.476581527446835	0.0	0.0	0.0	0.0
16	1.512139748096317	0.0	0.0	0.0	0.0
17	1.5566947530231026	0.0	0.0	0.0	0.0
18	1.6466625957961374	0.0	0.0	0.0	0.0
19	1.7022334307740865	0.0	0.0	0.0	0.0
20	1.7619678938245549	0.0	0.0	0.0	0.0
21	1.8201540730691619	0.0	0.0	0.0	1.0461377066631964E-5
22	1.8708289835799272	0.0	0.0	0.0	1.0461377066631964E-5
23	1.9388907027754347	0.0	0.0	0.0	5.2306885333159815E-5
24	2.006628119281877	0.0	0.0	0.0	5.2306885333159815E-5
25	2.076918111792577	0.0	0.0	0.0	5.2306885333159815E-5
26	2.1307418968003984	0.0	0.0	0.0	5.2306885333159815E-5
27	2.180663588162366	0.0	0.0	0.0	5.2306885333159815E-5
28	2.2432749299061583	0.0	0.0	0.0	5.2306885333159815E-5
29	2.3051121297470196	0.0	0.0	0.0	5.2306885333159815E-5
30	2.3804863515121033	0.0	0.0	0.0	5.2306885333159815E-5
31	2.454479671504391	0.0	0.0	0.0	5.2306885333159815E-5
32	2.5612275630923036	0.0	0.0	0.0	6.276826239979177E-5
33	2.618001456432915	0.0	0.0	0.0	6.276826239979177E-5
34	2.695059959905726	0.0	0.0	0.0	6.276826239979177E-5
35	2.743265985428766	0.0	0.0	0.0	6.276826239979177E-5
36	2.8041511999565643	0.0	0.0	0.0	6.276826239979177E-5
37	2.856855617618256	0.0	0.0	0.0	6.276826239979177E-5
38	2.9061705491103593	0.0	0.0	0.0	6.276826239979177E-5
39	2.959847874839248	0.0	0.0	0.0	6.276826239979177E-5
40	3.018462970543587	0.0	0.0	0.0	6.276826239979177E-5
41	3.092037835453209	0.0	0.0	0.0	6.276826239979177E-5
42	3.1917661430294118	0.0	0.0	0.0	6.276826239979177E-5
43	3.23960602035512	0.0	0.0	0.0	6.276826239979177E-5
44	3.2815142968840476	0.0	0.0	0.0	6.276826239979177E-5
45	3.321518602786848	0.0	0.0	0.0	6.276826239979177E-5
46	3.3624435098715124	0.0	0.0	0.0	6.276826239979177E-5
47	3.403807794792975	0.0	0.0	0.0	6.276826239979177E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTAG	45	7.6397555E-11	53.0	53
GCGACTA	235	0.0	49.61702	19
TCGGGGG	178585	0.0	48.50085	9
TCGGGAT	55525	0.0	48.48987	9
ATCGGGG	507790	0.0	48.48322	8
CTTACGT	170	0.0	48.323532	38
CGGGATT	14800	0.0	47.950676	10
ATCGGGC	101615	0.0	47.93549	8
CGGGGGC	48190	0.0	47.731895	10
CGGGGGG	73035	0.0	47.7098	10
CACGAGA	12290	0.0	47.393818	2
TATAGAG	7515	0.0	47.393215	1
AGAGATC	238530	0.0	47.36182	4
TCGGGGC	129910	0.0	47.327114	9
TAGAGAT	86400	0.0	47.31048	3
GATCGGG	1000815	0.0	47.224262	7
TCGGGGA	134325	0.0	47.188053	9
CTGAGAT	83195	0.0	47.135887	3
TACGGTA	90	0.0	47.11111	29
TGAGATC	380850	0.0	47.106472	4
>>END_MODULE