FastQCFastQC Report
Fri 17 Jun 2016
SRR1528083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434279
Sequences flagged as poor quality0
Sequence length52
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTATACAGGGGGCCGGGCGCGTGGCTCGCCGTGATCGTATAGTGGTTAGT33110.23084769420733345No Hit
AGACATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA29700.20707268251156158No Hit
GCCGATACAGGGAACGGATGCCTGTAAAAAACACGGCGGCTTCTACCTTGGC23440.16342705986771056No Hit
GCGTATACAGGGCTGGTAGGAATGCAAAACAGGGCTGGGCATGGTCGTTCAT19680.13721179770463068No Hit
GGGGATACAGGGGCTGTCTTCTTTATCATGCAACTCGTAGGACAGGTTCCGG19470.13574764742424592No Hit
TCTAATACAGGGGGCTTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTG16810.11720174387270538No Hit
ATAAATACAGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGC16780.11699257954693612No Hit
ACATATACAGGGGTAGCCCATTTCTCTCAGATCTGTAGATGTCTTCAAGTTT16580.11559815070847443No Hit
ACGAATACAGGGGGAGGCCAAGATGGGAAGAATACTTTGAGGCCAGGAATTC16410.114412886195782No Hit
TCAAATACAGGGGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAA16030.11176347140270478No Hit
GAGTATACAGGGACTGCCGAGCGTGATCAGATCGGCATTTCTTTAATCTTTT15690.1093929423773199No Hit
CAAAATACAGGGGGGGACAGTGGGTGTTGCTTGTAATCCCAGCACTTTGAGA15200.10597659172308874No Hit
GGTGATACAGGGGAAGTATTATTATTCATACTTGTAAATACTTTACAAATAT15030.1047913272103963No Hit
TTACATACAGGGGACTCTTCCAGCAAAGAAACAGACCAGATGCCGGGCGCGG14480.10095664790462663No Hit
TTAGATACAGGGGACCTCCCACTGGGGTCTAACCTGGGAACCCTCCGAGAAC14360.10011999060154962No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTG1400.046.00000421
CTGCTCG750.046.00000440
ATTTAAG351.0197982E-746.00000419
CTGCTAT1100.046.00000426
TGTTTAG351.0197982E-746.00000432
CGGCGTT551.8189894E-1246.00000445
ACTATGT1050.046.00000418
CGGCGAA750.046.00000426
TTGGCGG1050.046.00000436
TCTGACA551.8189894E-1246.00000446
TTGGCAC750.046.00000429
CCCCTAG551.8189894E-1246.00000440
CTCACAC750.046.00000431
TACCCGG551.8189894E-1246.00000425
GCCCAGC750.046.00000439
TCACCGG1100.046.00000430
TTGGACT750.046.00000445
CTCAACT1550.046.00000438
CTCAAAC351.0197982E-746.00000417
CAAGTAG351.0197982E-746.00000446
Produced by FastQC (version 0.11.5)