##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528083_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1434279
Sequences flagged as poor quality	0
Sequence length	52
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.937971621978708	31.0	31.0	34.0	28.0	34.0
2	31.689994066705292	31.0	31.0	34.0	30.0	34.0
3	31.92217832095429	31.0	31.0	34.0	30.0	34.0
4	35.55991616693823	37.0	35.0	37.0	33.0	37.0
5	35.47554764449595	37.0	35.0	37.0	33.0	37.0
6	35.42002357979166	37.0	35.0	37.0	33.0	37.0
7	35.634591317309955	37.0	35.0	37.0	33.0	37.0
8	35.730558001616146	37.0	35.0	37.0	33.0	37.0
9	37.388364467443225	39.0	37.0	39.0	34.0	39.0
10	37.22614568016404	39.0	37.0	39.0	33.0	39.0
11	37.61758486319607	39.0	37.0	39.0	35.0	39.0
12	37.570743906868884	39.0	37.0	39.0	35.0	39.0
13	37.321155089072626	39.0	37.0	39.0	35.0	39.0
14	37.43813163268792	40.0	37.0	40.0	32.0	40.0
15	36.336303466759254	38.0	35.0	40.0	31.0	40.0
16	36.70061961445437	39.0	36.0	40.0	31.0	40.0
17	36.41757356832248	39.0	35.0	40.0	30.0	40.0
18	36.65462159036004	39.0	35.0	40.0	31.0	40.0
19	36.79038666814476	39.0	36.0	40.0	31.0	40.0
20	36.823416504041404	39.0	35.0	40.0	31.0	40.0
21	36.910333345185975	39.0	36.0	40.0	31.0	40.0
22	37.040185347481206	39.0	36.0	40.0	31.0	40.0
23	37.09231397796384	39.0	36.0	40.0	32.0	40.0
24	37.106307071357804	39.0	36.0	40.0	32.0	40.0
25	37.23060436637502	40.0	36.0	40.0	32.0	40.0
26	36.99609211318021	39.0	36.0	40.0	31.0	40.0
27	36.673046178602625	39.0	35.0	40.0	31.0	40.0
28	36.27835727916256	38.0	35.0	40.0	30.0	40.0
29	36.13579296636149	38.0	35.0	40.0	30.0	40.0
30	35.96690392873353	38.0	35.0	40.0	30.0	40.0
31	35.83117162002651	38.0	35.0	40.0	30.0	40.0
32	35.78095754033908	38.0	35.0	40.0	30.0	40.0
33	35.61136431614769	38.0	35.0	40.0	29.0	40.0
34	35.45262672046373	38.0	35.0	40.0	28.0	40.0
35	35.341161656832455	38.0	35.0	40.0	27.0	40.0
36	35.07997188831462	38.0	34.0	40.0	26.0	40.0
37	34.867738424671906	37.0	34.0	40.0	25.0	40.0
38	34.46328991779145	37.0	33.0	40.0	23.0	40.0
39	33.900366665063075	36.0	33.0	40.0	21.0	40.0
40	33.37486430464366	35.0	33.0	40.0	19.0	40.0
41	32.73704139850057	35.0	33.0	39.0	15.0	40.0
42	32.11474406304492	35.0	32.0	39.0	12.0	40.0
43	31.533427596722813	35.0	31.0	38.0	10.0	40.0
44	31.0573988742776	35.0	30.0	38.0	10.0	40.0
45	30.684336868907653	35.0	29.0	38.0	9.0	40.0
46	30.311793591065616	35.0	27.0	37.0	9.0	40.0
47	29.735041090331798	35.0	25.0	37.0	8.0	40.0
48	29.339978483963023	35.0	23.0	36.0	8.0	40.0
49	29.076567390305513	35.0	23.0	36.0	8.0	40.0
50	28.68454115273249	34.0	23.0	35.0	8.0	39.0
51	28.306517072340878	34.0	20.0	35.0	8.0	39.0
52	27.474377021486056	33.0	18.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	7.0
14	8.0
15	27.0
16	53.0
17	192.0
18	570.0
19	1355.0
20	2565.0
21	4366.0
22	6887.0
23	10434.0
24	14930.0
25	20256.0
26	26903.0
27	35012.0
28	45389.0
29	57476.0
30	65544.0
31	65494.0
32	65426.0
33	75774.0
34	102268.0
35	136659.0
36	170129.0
37	255038.0
38	230707.0
39	40809.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.8379074085307	21.955142618695525	37.79759725966845	6.4093527131053305
2	27.350396959029588	20.381738838817274	33.08672859325139	19.181135608901755
3	26.78760547982645	22.514517747244433	32.781000070418656	17.91687670251046
4	20.672407530194615	24.419028654815417	32.87073156617367	22.037832248816304
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	100.0	0.0	0.0	0.0
11	98.53654693403445	1.2473165960039854	0.1717936328984807	0.04434283706308187
12	98.02569792906401	1.2949363408374521	0.44844831444928074	0.23091741564925652
13	91.35014875069635	5.459537509787148	1.9761148284259895	1.21419891109052
14	38.07613442015117	41.60027442359541	9.988363491343037	10.335227664910382
15	23.759603257106882	49.56113838381515	14.931265116480127	11.747993242597849
16	39.48722668323248	33.71373352046568	16.44707898532991	10.351960810971923
17	21.133057096980433	34.094412593365725	23.5195523325657	21.25297797708814
18	18.465166121793597	42.332279842345876	26.635682457876047	12.566871577984479
19	28.914667230015915	35.85111404405977	25.950529848097894	9.28368887782642
20	18.416709719657053	42.86153530798401	28.026137174148126	10.695617798210808
21	25.96963352318482	35.880397049667465	21.544622768652403	16.605346658495314
22	20.778732729127317	48.19180926444576	19.37447316735447	11.654984839072453
23	17.85064133268353	39.01179617075897	28.70724594029474	14.430316556262762
24	15.04484134537283	53.39498103228173	16.66495849133955	14.895219131005893
25	19.892852088052603	38.94681578688665	21.59586802846587	19.564464096594875
26	20.33174856495842	46.51026752814481	12.335256947915992	20.822726958980784
27	26.015789117737903	47.1554000302591	11.007272643606997	15.821538208395996
28	27.359321303595745	45.0943644855708	13.218557895639552	14.327756315193907
29	30.325968657422997	40.21490937258372	12.390336887035227	17.06878508295806
30	32.870940730499434	42.74084749201515	10.431791861973856	13.956419915511557
31	21.129292139116586	51.323068942653414	11.36829026988473	16.179348648345265
32	20.02295229868108	57.71248132336875	10.688715375460422	11.575851002489753
33	20.62708859294461	58.870833359478866	10.307896859676534	10.194181187899984
34	17.205927159220767	61.31540655618607	11.126217423527779	10.352448861065385
35	18.425912949990902	58.62973661330885	12.528106456275243	10.416243980425008
36	15.185330050847847	58.6906034321077	14.275534955193514	11.848531561850937
37	15.608818089088663	61.07166039522297	13.100449773021847	10.219071742666525
38	16.405315841617984	59.039001477397356	14.182387108784274	10.373295572200387
39	16.45356307942876	59.13786648204429	13.190948204638008	11.217622233888944
40	16.4902365578803	60.2369552925198	12.187098883829437	11.085709265770467
41	17.773459696474674	59.119669185702364	12.112008890878274	10.994862226944688
42	17.892753083605072	54.85201972559035	14.996803271887826	12.258423918916751
43	16.863315993610726	58.02309034713608	11.692355531943226	13.421238127309959
44	17.915621716555844	53.43848721204173	13.686737378153065	14.95915369324936
45	19.136165278861363	55.421434741776174	12.85056812516951	12.591831854192941
46	23.65564858719956	53.09022860963593	11.562115878430905	11.692006924733612
47	23.312619092937986	51.510619621426514	13.025220337186838	12.151540948448664
48	21.671027742858957	52.487138137001246	12.039986641371728	13.801847478768078
49	21.10293743406966	53.99737429049718	12.455386992349467	12.444301283083696
50	18.249936030577036	56.022363849711255	12.535496929119091	13.192203190592625
51	18.47304464473091	56.14779272373088	13.884676551772705	11.494486079765514
52	21.378127965340077	53.14356551270708	12.540168265727939	12.938138256224905
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.5
4	11.0
5	147.5
6	284.0
7	649.5
8	1015.0
9	4238.5
10	7462.0
11	11751.5
12	16041.0
13	16322.0
14	15390.0
15	14177.0
16	12227.0
17	10277.0
18	11445.5
19	12614.0
20	16040.0
21	19466.0
22	28010.5
23	36555.0
24	50247.0
25	63939.0
26	83722.0
27	103505.0
28	108967.5
29	114430.0
30	107336.0
31	100242.0
32	90571.5
33	80901.0
34	72435.0
35	63969.0
36	57738.5
37	51508.0
38	49368.0
39	50577.5
40	53927.0
41	59106.0
42	64285.0
43	65100.0
44	65915.0
45	68872.5
46	71830.0
47	75021.5
48	78213.0
49	73186.5
50	68160.0
51	66792.0
52	65424.0
53	58342.5
54	51261.0
55	44666.5
56	38072.0
57	31281.5
58	24491.0
59	25667.5
60	26844.0
61	25558.0
62	24272.0
63	19452.5
64	13624.0
65	12615.0
66	9211.0
67	5807.0
68	3836.5
69	1866.0
70	1705.0
71	1544.0
72	1167.0
73	790.0
74	508.5
75	227.0
76	2009.0
77	3791.0
78	1928.5
79	66.0
80	39.5
81	13.0
82	8.0
83	3.0
84	2.0
85	1.0
86	1.5
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1434279.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.63953096115672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.17128091280152	31.206357840059685
2	6.212129086357524	4.552189919885975
3	1.9875504286341157	2.1846874640040004
4	1.0139081075962673	1.4859647000016503
5	0.6733369375094731	1.2335374784584394
6	0.4602665189616483	1.0118369623127486
7	0.377477529636773	0.9681419743987248
8	0.30585326518392114	0.8965056143421725
9	0.2629212437109385	0.866997994436349
>10	3.0972635364627874	23.98404806973878
>50	0.25121944482554465	6.233886555680878
>100	0.14431358647447284	11.603328809472572
>500	0.030259299944387343	7.831378063770431
>1k	0.012220101900617966	5.941138553437631
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTATACAGGGGGCCGGGCGCGTGGCTCGCCGTGATCGTATAGTGGTTAGT	3311	0.23084769420733345	No Hit
AGACATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA	2970	0.20707268251156158	No Hit
GCCGATACAGGGAACGGATGCCTGTAAAAAACACGGCGGCTTCTACCTTGGC	2344	0.16342705986771056	No Hit
GCGTATACAGGGCTGGTAGGAATGCAAAACAGGGCTGGGCATGGTCGTTCAT	1968	0.13721179770463068	No Hit
GGGGATACAGGGGCTGTCTTCTTTATCATGCAACTCGTAGGACAGGTTCCGG	1947	0.13574764742424592	No Hit
TCTAATACAGGGGGCTTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTG	1681	0.11720174387270538	No Hit
ATAAATACAGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGC	1678	0.11699257954693612	No Hit
ACATATACAGGGGTAGCCCATTTCTCTCAGATCTGTAGATGTCTTCAAGTTT	1658	0.11559815070847443	No Hit
ACGAATACAGGGGGAGGCCAAGATGGGAAGAATACTTTGAGGCCAGGAATTC	1641	0.114412886195782	No Hit
TCAAATACAGGGGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAA	1603	0.11176347140270478	No Hit
GAGTATACAGGGACTGCCGAGCGTGATCAGATCGGCATTTCTTTAATCTTTT	1569	0.1093929423773199	No Hit
CAAAATACAGGGGGGGACAGTGGGTGTTGCTTGTAATCCCAGCACTTTGAGA	1520	0.10597659172308874	No Hit
GGTGATACAGGGGAAGTATTATTATTCATACTTGTAAATACTTTACAAATAT	1503	0.1047913272103963	No Hit
TTACATACAGGGGACTCTTCCAGCAAAGAAACAGACCAGATGCCGGGCGCGG	1448	0.10095664790462663	No Hit
TTAGATACAGGGGACCTCCCACTGGGGTCTAACCTGGGAACCCTCCGAGAAC	1436	0.10011999060154962	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.2993838716177257	0.0	0.0	0.0	0.0
10	0.2993838716177257	0.0	0.0	0.0	0.0
11	0.3768443935942728	0.0	0.0	0.0	0.0
12	0.49606805928274766	0.0	0.0	0.0	0.0
13	0.5626520363192935	0.0	0.0	0.0	0.0
14	0.9762396298070319	0.0	0.0	0.0	0.0
15	1.300583777633222	0.0	0.0	0.0	0.0
16	1.4838814484490117	0.0	0.0	0.0	0.0
17	1.6228362822017195	0.0	0.0	0.0	0.0
18	1.8310942292259735	0.0	0.0	0.0	0.0
19	1.9729076420975278	0.0	0.0	0.0	0.0
20	2.129571722098699	0.0	0.0	0.0	0.0
21	2.2803792009783312	0.0	0.0	0.0	0.0
22	2.4672326653321983	0.0	0.0	0.0	0.0
23	2.6335880257606785	0.0	0.0	0.0	0.0
24	2.84428622325224	0.0	0.0	0.0	0.0
25	3.0520561201830327	0.0	0.0	0.0	0.0
26	3.2898062371407515	0.0	0.0	0.0	0.0
27	3.5002255488646212	0.0	0.0	0.0	0.0
28	3.7200572552481073	0.0	0.0	0.0	0.0
29	3.968474752820058	0.0	0.0	0.0	0.0
30	4.256215143636629	0.0	0.0	0.0	0.0
31	4.548905756829738	0.0	0.0	0.0	0.0
32	4.795719661237458	0.0	0.0	0.0	0.0
33	5.013599167247098	0.0	0.0	0.0	0.0
34	5.223809314645198	0.0	0.0	0.0	0.0
35	5.421051273845604	0.0	0.0	0.0	0.0
36	5.64018576580986	0.0	0.0	0.0	0.0
37	5.860993572380269	0.0	0.0	0.0	0.0
38	6.077130042341832	0.0	0.0	0.0	0.0
39	6.325965868565321	0.0	0.0	0.0	0.0
40	6.595857570249581	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTG	140	0.0	46.000004	21
CTGCTCG	75	0.0	46.000004	40
ATTTAAG	35	1.0197982E-7	46.000004	19
CTGCTAT	110	0.0	46.000004	26
TGTTTAG	35	1.0197982E-7	46.000004	32
CGGCGTT	55	1.8189894E-12	46.000004	45
ACTATGT	105	0.0	46.000004	18
CGGCGAA	75	0.0	46.000004	26
TTGGCGG	105	0.0	46.000004	36
TCTGACA	55	1.8189894E-12	46.000004	46
TTGGCAC	75	0.0	46.000004	29
CCCCTAG	55	1.8189894E-12	46.000004	40
CTCACAC	75	0.0	46.000004	31
TACCCGG	55	1.8189894E-12	46.000004	25
GCCCAGC	75	0.0	46.000004	39
TCACCGG	110	0.0	46.000004	30
TTGGACT	75	0.0	46.000004	45
CTCAACT	155	0.0	46.000004	38
CTCAAAC	35	1.0197982E-7	46.000004	17
CAAGTAG	35	1.0197982E-7	46.000004	46
>>END_MODULE