FastQCFastQC Report
Fri 17 Jun 2016
SRR1528082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423678
Sequences flagged as poor quality0
Sequence length52
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTATACAGGGGGCCGGGCGCGTGGCTCGCCGTGATCGTATAGTGGTTAGT33120.23263687434939645No Hit
AGACATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA28350.19913210711972792No Hit
GCCGATACAGGGAACGGATGCCTGTAAAAAACACGGCGGCTTCTACCTTGGC21670.1522113848777603No Hit
GCGTATACAGGGCTGGTAGGAATGCAAAACAGGGCTGGGCATGGTCGTTCAT20040.14076216672590292No Hit
GGGGATACAGGGGCTGTCTTCTTTATCATGCAACTCGTAGGACAGGTTCCGG19070.1339488283165154No Hit
ACGAATACAGGGGGAGGCCAAGATGGGAAGAATACTTTGAGGCCAGGAATTC17090.12004118908910581No Hit
ATAAATACAGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGC16650.11695060259412592No Hit
TCTAATACAGGGGGCTTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTG16640.11688036199196729No Hit
ACATATACAGGGGTAGCCCATTTCTCTCAGATCTGTAGATGTCTTCAAGTTT16370.11498386573368417No Hit
TCAAATACAGGGGCGTCAGACGCTAAGCCAGCAAAATACGCCGACGCTGGAA15800.110980151410642No Hit
CAAAATACAGGGGGGGACAGTGGGTGTTGCTTGTAATCCCAGCACTTTGAGA15520.10901341455020026No Hit
GAGTATACAGGGACTGCCGAGCGTGATCAGATCGGCATTTCTTTAATCTTTT15240.10704667768975851No Hit
GGTGATACAGGGGAAGTATTATTATTCATACTTGTAAATACTTTACAAATAT14990.10529066263579263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC351.0197982E-746.00000431
TCCGGCC551.8189894E-1246.00000423
TCACGCG551.8189894E-1246.00000421
TCTGCGA700.046.00000429
ATTTAAG351.0197982E-746.00000419
ACAACAT351.0197982E-746.00000431
CTAGAGA700.046.00000422
TCCATTC351.0197982E-746.00000436
GACAGCT700.046.00000441
GACAGAC700.046.00000423
CCCCTCG551.8189894E-1246.00000435
CTCACAC700.046.00000431
TACCCGG351.0197982E-746.00000425
CGAACAT551.8189894E-1246.00000433
ATGATTT351.0197982E-746.00000437
CGTATTA551.8189894E-1246.00000435
CCCTCGC551.8189894E-1246.00000436
GCGACAG1100.046.00000437
ATAGGTT551.8189894E-1246.00000445
CGCGGTA351.0197982E-746.00000435
Produced by FastQC (version 0.11.5)