FastQCFastQC Report
Fri 17 Jun 2016
SRR1528081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368581
Sequences flagged as poor quality0
Sequence length59
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA27390.20013429968704813No Hit
GGCTATACAGGGGGCCGGGCGCGTGGCTCGCCGTGATCGTATAGTGGTTAGTACTCTGC25360.18530141803809932No Hit
GCCGATACAGGGAACGGATGCCTGTAAAAAACACGGCGGCTTCTACCTTGGCAGTATCG18780.1372224223484032No Hit
GCGTATACAGGGCTGGTAGGAATGCAAAACAGGGCTGGGCATGGTCGTTCATGCCTGTA16460.12027055760674742No Hit
GGGGATACAGGGGCTGTCTTCTTTATCATGCAACTCGTAGGACAGGTTCCGGCAGCGCT15080.11018712082076253No Hit
ACATATACAGGGGTAGCCCATTTCTCTCAGATCTGTAGATGTCTTCAAGTTTTTGGCCT14940.1091641634656626No Hit
ACGAATACAGGGGGAGGCCAAGATGGGAAGAATACTTTGAGGCCAGGAATTCAAGACCA14270.10426858183768443No Hit
GGTGATACAGGGGAAGTATTATTATTCATACTTGTAAATACTTTACAAATATAGTAAAT13880.10141891491990608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAGC650.053.00000450
AACGTAT503.6379788E-1253.00000433
CTATGAA503.6379788E-1253.00000433
TAATAAG251.4788815E-553.00000432
GTCGCGG353.342757E-853.00000443
CATATTC700.053.00000438
GCGACGA700.053.00000444
CATATGT650.053.00000441
TACTTTG1750.053.00000433
CAAGTTA353.342757E-853.00000441
CATATAA700.053.00000444
TTCAGGC2000.053.00000420
CATGCTT251.4788815E-553.00000442
TTTACCG700.053.00000418
CACGGCG1750.053.00000432
CGAAACT700.053.00000448
CGACGGT503.6379788E-1253.00000434
CGACGAA700.053.00000445
CCGATAA503.6379788E-1253.00000430
CCTAGAC700.053.00000429