##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528081_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1368581
Sequences flagged as poor quality	0
Sequence length	59
%GC	37
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.50998150639239	31.0	31.0	33.0	27.0	34.0
2	31.33760734658745	31.0	31.0	34.0	28.0	34.0
3	31.58896404377965	31.0	31.0	34.0	30.0	34.0
4	35.301782649328025	37.0	35.0	37.0	33.0	37.0
5	35.07248529681473	37.0	35.0	37.0	32.0	37.0
6	35.042159726022795	37.0	35.0	37.0	32.0	37.0
7	35.18690380766648	37.0	35.0	37.0	32.0	37.0
8	35.4174170180647	37.0	35.0	37.0	33.0	37.0
9	36.95348759043126	39.0	37.0	39.0	33.0	39.0
10	36.8341435399147	39.0	37.0	39.0	33.0	39.0
11	37.18192565876627	39.0	37.0	39.0	34.0	39.0
12	37.17659166684325	39.0	37.0	39.0	34.0	39.0
13	36.80934997636238	39.0	37.0	39.0	32.0	39.0
14	36.609608053889396	39.0	36.0	40.0	31.0	40.0
15	35.499654021208826	38.0	34.0	40.0	27.0	40.0
16	35.74207080180128	38.0	34.0	40.0	28.0	40.0
17	35.59552923794792	38.0	34.0	40.0	27.0	40.0
18	35.91532616629925	38.0	34.0	40.0	29.0	40.0
19	36.16342985910224	38.0	35.0	40.0	30.0	40.0
20	36.12538315233077	38.0	35.0	40.0	30.0	40.0
21	36.19462421296218	38.0	35.0	40.0	30.0	40.0
22	36.241200922707534	38.0	35.0	40.0	30.0	40.0
23	36.27602166039131	38.0	35.0	40.0	30.0	40.0
24	36.322975402990394	38.0	35.0	40.0	30.0	40.0
25	36.388565967231756	39.0	35.0	40.0	30.0	40.0
26	35.972191634985435	38.0	35.0	40.0	29.0	40.0
27	35.6034936916412	38.0	34.0	40.0	27.0	40.0
28	35.197248098578015	37.0	34.0	40.0	27.0	40.0
29	34.972270548838544	37.0	34.0	40.0	27.0	40.0
30	34.68528936175498	37.0	33.0	40.0	26.0	40.0
31	34.58376668973192	37.0	33.0	40.0	25.0	40.0
32	34.55840246211222	37.0	33.0	40.0	25.0	40.0
33	34.25166139234726	37.0	33.0	40.0	24.0	40.0
34	34.08343313256577	36.0	33.0	40.0	23.0	40.0
35	34.00849200741498	36.0	33.0	40.0	23.0	40.0
36	33.5876919232402	36.0	33.0	40.0	21.0	40.0
37	33.52383600239956	36.0	33.0	40.0	21.0	40.0
38	33.284477864298864	35.0	32.0	40.0	21.0	40.0
39	32.63741203480101	35.0	31.0	39.0	18.0	40.0
40	32.14363051949428	35.0	31.0	39.0	15.0	40.0
41	31.464611886326058	35.0	30.0	38.0	12.0	40.0
42	30.93537393840774	35.0	29.0	38.0	10.0	40.0
43	30.428181452175647	35.0	28.0	37.0	10.0	40.0
44	29.948816328737575	34.0	26.0	37.0	9.0	40.0
45	29.608661818335925	34.0	25.0	36.0	9.0	40.0
46	29.207968691659463	34.0	23.0	36.0	8.0	40.0
47	28.58426063199767	33.0	23.0	35.0	8.0	39.0
48	28.171256944236404	33.0	22.0	35.0	8.0	39.0
49	27.814757036667906	33.0	20.0	35.0	8.0	39.0
50	27.226585054154633	33.0	20.0	35.0	8.0	38.0
51	26.84953831742513	33.0	18.0	35.0	8.0	38.0
52	26.16031349258831	32.0	15.0	35.0	8.0	38.0
53	25.82245625213268	32.0	13.0	35.0	8.0	38.0
54	25.407214479815224	31.0	12.0	35.0	8.0	37.0
55	24.92214344638717	31.0	10.0	35.0	8.0	37.0
56	24.2165147696775	30.0	10.0	35.0	8.0	36.0
57	23.413958691520634	27.0	9.0	35.0	8.0	36.0
58	22.697938229450795	26.0	9.0	34.0	8.0	35.0
59	21.73314769092951	24.0	8.0	34.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	24.0
15	90.0
16	349.0
17	967.0
18	2431.0
19	4880.0
20	8427.0
21	13158.0
22	18860.0
23	26256.0
24	34116.0
25	44241.0
26	54841.0
27	63409.0
28	63645.0
29	60387.0
30	64261.0
31	75181.0
32	88754.0
33	104617.0
34	125593.0
35	133277.0
36	144175.0
37	142047.0
38	89557.0
39	5033.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.30918593784365	22.482556750385985	36.94373953752098	6.264517774249387
2	26.99401789152414	21.07365219888337	32.638185098287934	19.294144811304555
3	26.699333104872856	22.835477037895455	32.56935468196621	17.895835175265475
4	20.69464649881885	24.654295215263108	32.624009832081555	22.027048453836493
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	100.0	0.0	0.0	0.0
11	98.55412284694877	1.199928977532203	0.18778574304334197	0.05816243247568102
12	98.11454345778584	1.2255759797922081	0.4138593185204237	0.24602124390153013
13	91.39042555756656	5.546474779351752	1.8795380032310838	1.1835616598506045
14	37.87499607257444	41.89602223032469	10.043249175605974	10.185732521494892
15	23.512017191529036	50.030067639401686	14.861086044596556	11.59682912447272
16	39.68943014699167	33.32524709900254	16.539685995933016	10.445636758072778
17	21.05056259001111	33.79120417425055	23.662172717581203	21.49606051815713
18	18.42894209403755	42.29424491498859	26.62378039736048	12.653032593613384
19	29.292822273581177	35.67943731499999	25.849036337637305	9.17870407378153
20	18.271845071647203	42.810765310931544	28.231430949282505	10.68595866813875
21	26.119827763208754	35.60300778689752	21.920587820523593	16.356576629370128
22	20.81915502261101	48.188305989926796	19.544477089774006	11.44806189768819
23	17.67896821598429	38.87362165629948	29.295891145646475	14.15151898206975
24	14.745345726705253	53.89867315124205	16.759841032427016	14.596140089625678
25	19.677242340789476	38.88648169162074	21.860525610102727	19.575750357487063
26	20.04821051877821	46.75543500896184	12.34687607090848	20.849478401351472
27	25.848524858959752	47.342247188876655	11.061384017460421	15.747843934703171
28	27.25202235015684	45.5152453526682	13.103791445299912	14.128940851875043
29	30.34113435741107	40.30276614975657	12.253860019976896	17.10223947285546
30	33.0043307630312	42.93476235604615	10.280136871694111	13.780770009228538
31	20.84187928957073	51.89060786318092	11.179608660356969	16.087904186891386
32	19.688421803313066	58.63138535461182	10.360000613774414	11.3201922283007
33	20.514606004321266	59.61254759491765	9.9912975556434	9.88154884511768
34	16.83502839802686	62.30241396015289	11.000591123214482	9.861966518605767
35	18.218359015651977	59.3631652054208	12.327220676014061	10.091255102913163
36	14.842599743822252	59.42030468054138	14.27580830071439	11.46128727492198
37	15.291897227858636	62.16731052089719	12.680506305436069	9.860285945808103
38	15.97552501459541	60.22281472561727	13.707555489956386	10.094104769830942
39	15.986850613884016	60.38765699655336	12.915348086814008	10.710144302748613
40	15.841590669459826	61.656927869084846	11.8341552308559	10.66732623059943
41	16.98467244540148	60.60744669113484	11.735001435793716	10.672879427669974
42	16.898524822425564	56.554708855376475	14.746295615677846	11.80047070652011
43	16.045816798567277	59.79368411515284	11.129191476427044	13.031307609852833
44	17.15031846854516	54.92214198501952	13.16129626233303	14.766243284102293
45	18.402929749865006	57.16366075519096	12.34183435251549	12.091575142428544
46	23.094431385500748	54.40525624716403	10.994745652613911	11.505566714721306
47	22.74911020977202	52.75157261426251	12.551248336780944	11.948068839184527
48	21.160968915979396	53.68246380740344	11.546265803777782	13.610301472839387
49	20.51109872196092	55.160271843610275	12.1330779836926	12.1955514507362
50	17.46465865009086	57.38198908212229	12.191532689698308	12.961819578088546
51	17.79237034563537	57.357072763687356	13.570698409520517	11.27985848115676
52	19.33513617389106	55.186722598077864	12.599035058940611	12.879106169090468
53	16.97086252110763	55.3140077204053	13.71508153335462	14.000048225132456
54	17.97357993425307	53.782713628203226	14.166498000483713	14.07720843705999
55	18.095165722744948	51.79167327326625	14.492529123230558	15.620631880758246
56	19.236201583976396	50.395336483554864	15.133119632670628	15.235342299798113
57	20.131800748366373	47.509573784818	15.80118385393338	16.557441612882247
58	20.642475673708756	46.24169121155416	16.007236692603506	17.10859642213358
59	22.671438519166934	42.68092279521636	17.814217792005003	16.833420893611702
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.0
4	101.0
5	192.0
6	502.0
7	812.0
8	3149.5
9	8727.5
10	11968.0
11	12023.0
12	12078.0
13	10836.5
14	7513.5
15	5432.0
16	5304.5
17	5177.0
18	5889.0
19	9297.0
20	11993.0
21	17459.5
22	22926.0
23	35192.0
24	47458.0
25	59513.5
26	77581.0
27	83593.0
28	87695.0
29	91797.0
30	90779.0
31	85172.5
32	80584.0
33	76356.5
34	72129.0
35	68410.5
36	61353.5
37	58015.0
38	54874.5
39	51734.0
40	51875.0
41	52016.0
42	50887.0
43	53899.0
44	58040.0
45	55889.0
46	53738.0
47	53448.0
48	51809.0
49	50460.0
50	49316.5
51	48173.0
52	42169.0
53	37443.5
54	38722.0
55	32669.0
56	26616.0
57	21987.0
58	18972.5
59	20587.0
60	20922.5
61	21258.0
62	17799.5
63	14341.0
64	10277.0
65	6690.0
66	7167.0
67	5427.5
68	3688.0
69	2646.0
70	1022.5
71	441.0
72	1141.5
73	1842.0
74	1535.0
75	1337.0
76	1446.0
77	1169.0
78	892.0
79	473.0
80	54.0
81	31.5
82	5.5
83	2.0
84	2.0
85	2.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1368581.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.868039874271034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.8899219455177	49.869518614760466
2	4.294129486557314	4.712209357874193
3	1.3185492869430153	2.17038644556543
4	0.7060765981625231	1.5496415576908384
5	0.419347169623873	1.150437861204268
6	0.29315924003982113	0.9651043723209539
7	0.23368797609633787	0.8975400833414094
8	0.17049267893040768	0.7483679284659918
9	0.15608827672454348	0.7707832012105652
>10	1.3406328954526425	14.712202285180703
>50	0.08261510123069667	3.0182988331232945
>100	0.0718516004950262	9.009335657131217
>500	0.019334104888258305	7.440065862986778
>1k	0.004113639337927299	2.98610793914395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGACATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	2739	0.20013429968704813	No Hit
GGCTATACAGGGGGCCGGGCGCGTGGCTCGCCGTGATCGTATAGTGGTTAGTACTCTGC	2536	0.18530141803809932	No Hit
GCCGATACAGGGAACGGATGCCTGTAAAAAACACGGCGGCTTCTACCTTGGCAGTATCG	1878	0.1372224223484032	No Hit
GCGTATACAGGGCTGGTAGGAATGCAAAACAGGGCTGGGCATGGTCGTTCATGCCTGTA	1646	0.12027055760674742	No Hit
GGGGATACAGGGGCTGTCTTCTTTATCATGCAACTCGTAGGACAGGTTCCGGCAGCGCT	1508	0.11018712082076253	No Hit
ACATATACAGGGGTAGCCCATTTCTCTCAGATCTGTAGATGTCTTCAAGTTTTTGGCCT	1494	0.1091641634656626	No Hit
ACGAATACAGGGGGAGGCCAAGATGGGAAGAATACTTTGAGGCCAGGAATTCAAGACCA	1427	0.10426858183768443	No Hit
GGTGATACAGGGGAAGTATTATTATTCATACTTGTAAATACTTTACAAATATAGTAAAT	1388	0.10141891491990608	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.26165787775805743	0.0	0.0	0.0	0.0
10	0.26165787775805743	0.0	0.0	0.0	0.0
11	0.332388072024966	0.0	0.0	0.0	0.0
12	0.4321264141472079	0.0	0.0	0.0	0.0
13	0.49123873559548176	0.0	0.0	0.0	0.0
14	0.8570190584262093	0.0	0.0	0.0	0.0
15	1.1360672112209653	0.0	0.0	0.0	0.0
16	1.2950640115564953	0.0	0.0	0.0	0.0
17	1.4127041073929858	0.0	0.0	0.0	0.0
18	1.5922331232130214	0.0	0.0	0.0	0.0
19	1.7149149374425043	0.0	0.0	0.0	0.0
20	1.8542563428836145	0.0	0.0	0.0	0.0
21	1.9905288762594249	0.0	0.0	0.0	0.0
22	2.153763642780369	0.0	0.0	0.0	0.0
23	2.2927397063089434	0.0	0.0	0.0	0.0
24	2.470149739036272	0.0	0.0	0.0	0.0
25	2.6330922320271872	0.0	0.0	0.0	0.0
26	2.829938454501414	0.0	0.0	0.0	0.0
27	3.013413162976835	0.0	0.0	0.0	0.0
28	3.208213470740862	0.0	0.0	0.0	0.0
29	3.423180652076859	0.0	0.0	0.0	0.0
30	3.6692018959783894	0.0	0.0	0.0	0.0
31	3.9196803112128547	0.0	0.0	0.0	0.0
32	4.137643296231644	0.0	0.0	0.0	0.0
33	4.3274749539851864	0.0	0.0	0.0	0.0
34	4.509488294810464	0.0	0.0	0.0	0.0
35	4.681052856937222	0.0	0.0	0.0	0.0
36	4.858974368342101	0.0	0.0	0.0	0.0
37	5.0510711459533635	0.0	0.0	0.0	0.0
38	5.244921564744797	0.0	0.0	0.0	0.0
39	5.463103754911109	0.0	0.0	0.0	0.0
40	5.701598955414403	0.0	0.0	0.0	0.0
41	5.933225727962028	0.0	0.0	0.0	0.0
42	6.176689578475808	0.0	0.0	0.0	0.0
43	6.422126275317281	0.0	0.0	0.0	0.0
44	6.663617279503369	0.0	0.0	0.0	0.0
45	6.92593277270399	0.0	0.0	0.0	0.0
46	7.15412533127378	0.0	0.0	0.0	0.0
47	7.369165580992283	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGAGC	65	0.0	53.000004	50
AACGTAT	50	3.6379788E-12	53.000004	33
CTATGAA	50	3.6379788E-12	53.000004	33
TAATAAG	25	1.4788815E-5	53.000004	32
GTCGCGG	35	3.342757E-8	53.000004	43
CATATTC	70	0.0	53.000004	38
GCGACGA	70	0.0	53.000004	44
CATATGT	65	0.0	53.000004	41
TACTTTG	175	0.0	53.000004	33
CAAGTTA	35	3.342757E-8	53.000004	41
CATATAA	70	0.0	53.000004	44
TTCAGGC	200	0.0	53.000004	20
CATGCTT	25	1.4788815E-5	53.000004	42
TTTACCG	70	0.0	53.000004	18
CACGGCG	175	0.0	53.000004	32
CGAAACT	70	0.0	53.000004	48
CGACGGT	50	3.6379788E-12	53.000004	34
CGACGAA	70	0.0	53.000004	45
CCGATAA	50	3.6379788E-12	53.000004	30
CCTAGAC	70	0.0	53.000004	29
>>END_MODULE