FastQCFastQC Report
Fri 17 Jun 2016
SRR1528080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986245
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGATCTCTGGGGCGAGTCCCGCTGGGCCGCAGCCGGTGGCCGGCTCGCCG44150.14784453385438906No Hit
AATTATCTCTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATC37480.1255087911407135No Hit
GTCCATCTCTGGGGGCCGCCCATCCCTCTTTGTGTGCTTGGGACGCCGCGGA37020.1239683950914945No Hit
GGGCATCTCTGGGATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG35400.1185435220485928No Hit
AGCCATCTCTGGGGGGGCCGCCTCCGTCTCCTTGAGGAGATGCACCCAGGGG35020.11727102096445537No Hit
TATAATCTCTGGGTCTGACCCTGATCTCAGCCTATTCCTAAGCTTGGCCCTC34180.11445812383109892No Hit
GTCGATCTCTGGGTTGATACCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAA33200.11117641050884974No Hit
ACGGATCTCTGGGGCTCTCTTTCCCGCCTTGCAAGATGGCGGGTGAAAAAGC32870.1100713437778883No Hit
AGAAATCTCTGGGGGCGGACACCAGGACTTCAAGATGGCGTCACTCGTGCCG32400.10849746085803409No Hit
TCCTATCTCTGGGATCGAGCCGCTGCCGCTCCCCTGGCATAGCCTGCTTCCT31780.10642127487865195No Hit
CAGCATCTCTGGGGCTCTGACCCTGATCTCAGCCTATTCCTAAGCTTGGCCC31720.10622035365484078No Hit
TCATATCTCTGGGGTGAGCATAGTGTCCGGCCACTGCACGCAGTCAGGCATG30340.10159916550718376No Hit
CTCTATCTCTGGGTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCT30180.10106337557702064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGA700.046.00000416
GTCATAC351.02030754E-746.00000413
TTCTACG351.02030754E-746.00000443
GATACGG351.02030754E-746.00000416
CCTTAAA351.02030754E-746.00000441
AATTTAG351.02030754E-746.00000418
ACGTTGT700.046.00000425
TTTAACC1400.046.00000420
ATTACGA351.02030754E-746.00000431
CGCGCAA700.046.00000423
GGTCATA351.02030754E-746.00000412
CAATTGA1050.046.00000435
CGACAAG1050.046.00000432
ATACTTA351.02030754E-746.00000436
GTAATGC700.046.00000444
CATTACG351.02030754E-746.00000430
CTAACTA351.02030754E-746.00000433
CTAACCG351.02030754E-746.00000424
GTTCGTT1050.046.00000438
CTTATAC351.02030754E-746.00000440
Produced by FastQC (version 0.11.5)