FastQCFastQC Report
Fri 17 Jun 2016
SRR1528079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2965096
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGATCTCTGGGGCGAGTCCCGCTGGGCCGCAGCCGGTGGCCGGCTCGCCG42660.1438739251612764No Hit
GTCCATCTCTGGGGGCCGCCCATCCCTCTTTGTGTGCTTGGGACGCCGCGGA37550.1266400818051085No Hit
AATTATCTCTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATC36620.12350358976572766No Hit
GGGCATCTCTGGGATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG34640.11682589703672326No Hit
TATAATCTCTGGGTCTGACCCTGATCTCAGCCTATTCCTAAGCTTGGCCCTC33960.11453254801868135No Hit
AGCCATCTCTGGGGGGGCCGCCTCCGTCTCCTTGAGGAGATGCACCCAGGGG33880.11426274225185289No Hit
AGAAATCTCTGGGGGCGGACACCAGGACTTCAAGATGGCGTCACTCGTGCCG32810.11065409012052224No Hit
ACGGATCTCTGGGGCTCTCTTTCCCGCCTTGCAAGATGGCGGGTGAAAAAGC32770.110519187237108No Hit
TCCTATCTCTGGGATCGAGCCGCTGCCGCTCCCCTGGCATAGCCTGCTTCCT32340.10906898124040502No Hit
GTCGATCTCTGGGTTGATACCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAA32230.10869799831101588No Hit
CAGCATCTCTGGGGCTCTGACCCTGATCTCAGCCTATTCCTAAGCTTGGCCC30780.10380776878725007No Hit
CATCATCTCTGGGAGACGCTAGCTGTAGCTGGCAGGCGGTTGTACGTGCTCC30780.10380776878725007No Hit
CTCTATCTCTGGGTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCT30060.10137951688579391No Hit
AGATATCTCTGGGGCCTTTGCGGGCGCCTCTGGATCCCGCGGCCTCTTCTGG29760.10036774526018719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGG351.02030754E-746.00000436
CTAGATT700.046.00000446
ACAACCC1050.046.00000428
GACAGTC750.046.00000440
CCTTACG750.046.00000439
CACGGTT750.046.00000438
CGAAAGT351.02030754E-746.00000444
TTAGCCT750.046.00000425
TAGACGA700.046.00000446
GCGAACC1150.046.00000419
AACCAAT351.02030754E-746.00000431
ATAGCCG1050.046.00000414
ACTTCTA750.046.00000427
TAAACCC1400.046.00000426
ATATGTT351.02030754E-746.00000437
GTCTAGT1400.046.00000432
AAGGTAC750.046.00000427
TACGGCA750.046.00000442
TAGCCGA351.02030754E-746.00000420
ATGTACC750.046.00000428
Produced by FastQC (version 0.11.5)