##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2965096
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46967147100802	31.0	31.0	34.0	30.0	34.0
2	31.985303342623645	31.0	31.0	34.0	30.0	34.0
3	32.17059211573588	33.0	31.0	34.0	30.0	34.0
4	35.801697145724795	37.0	35.0	37.0	35.0	37.0
5	35.6347595491006	37.0	35.0	37.0	33.0	37.0
6	35.76624972682166	37.0	35.0	37.0	33.0	37.0
7	35.647344976351526	37.0	35.0	37.0	33.0	37.0
8	35.73619707422626	37.0	35.0	37.0	33.0	37.0
9	37.442978911981264	39.0	37.0	39.0	34.0	39.0
10	37.65024977268864	39.0	37.0	39.0	35.0	39.0
11	37.502374965262504	39.0	37.0	39.0	35.0	39.0
12	37.296973521262046	39.0	37.0	39.0	35.0	39.0
13	37.26652965030475	39.0	37.0	39.0	35.0	39.0
14	37.778693506044995	40.0	38.0	40.0	33.0	40.0
15	37.21071122149165	39.0	37.0	40.0	32.0	40.0
16	36.910842684351536	39.0	36.0	40.0	31.0	40.0
17	36.753169205988605	39.0	36.0	40.0	31.0	40.0
18	36.81783658943926	39.0	36.0	40.0	31.0	40.0
19	37.05443196442881	39.0	36.0	40.0	32.0	40.0
20	37.241431643359945	39.0	36.0	40.0	32.0	40.0
21	37.19073109268638	39.0	36.0	40.0	32.0	40.0
22	37.196823981415776	39.0	36.0	40.0	32.0	40.0
23	37.264087233600534	39.0	36.0	40.0	32.0	40.0
24	37.23905027021048	39.0	36.0	40.0	32.0	40.0
25	37.23524803244145	40.0	36.0	40.0	32.0	40.0
26	36.96134863761578	40.0	36.0	40.0	31.0	40.0
27	36.75663351203469	39.0	35.0	40.0	31.0	40.0
28	36.74195439203317	39.0	35.0	40.0	31.0	40.0
29	36.79497088795776	39.0	35.0	40.0	31.0	40.0
30	36.64231141251413	39.0	35.0	40.0	31.0	40.0
31	36.70098978245561	39.0	35.0	40.0	31.0	40.0
32	36.766901307748554	39.0	35.0	40.0	31.0	40.0
33	36.77735864201362	39.0	35.0	40.0	31.0	40.0
34	36.73796632554224	39.0	35.0	40.0	31.0	40.0
35	36.49782098117565	39.0	35.0	40.0	31.0	40.0
36	36.52101786923593	39.0	35.0	40.0	31.0	40.0
37	36.40020154490782	38.0	35.0	40.0	31.0	40.0
38	36.202723284507485	38.0	35.0	40.0	30.0	40.0
39	36.02940714229826	38.0	35.0	40.0	30.0	40.0
40	35.87536086521313	38.0	35.0	40.0	30.0	40.0
41	35.72140868288919	38.0	35.0	40.0	30.0	40.0
42	35.506044998205795	38.0	34.0	40.0	29.0	40.0
43	35.38262066388407	37.0	34.0	40.0	29.0	40.0
44	35.10070938681243	37.0	34.0	40.0	28.0	40.0
45	34.96685975766046	37.0	34.0	40.0	27.0	40.0
46	34.7918671098676	36.0	33.0	40.0	27.0	40.0
47	34.62470253914207	36.0	33.0	40.0	27.0	40.0
48	34.41718784147292	36.0	33.0	39.0	26.0	40.0
49	34.10586200244444	35.0	33.0	39.0	26.0	40.0
50	33.90751597924654	35.0	33.0	39.0	24.0	40.0
51	33.72661323613131	35.0	33.0	39.0	24.0	40.0
52	32.449904151501336	35.0	31.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	1.0
13	7.0
14	5.0
15	16.0
16	44.0
17	138.0
18	392.0
19	873.0
20	1737.0
21	3149.0
22	5214.0
23	8240.0
24	12254.0
25	17612.0
26	25009.0
27	33403.0
28	43903.0
29	56664.0
30	72124.0
31	89419.0
32	110405.0
33	139992.0
34	196534.0
35	291739.0
36	307187.0
37	515710.0
38	833404.0
39	199917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.844799628747264	21.36163550859736	33.5974956628723	16.196069199783075
2	19.157457296492257	24.25816904410515	35.10520401363058	21.479169645772007
3	21.264235626772287	22.300188594231013	35.17983903387951	21.25573674511719
4	20.956859406912965	19.92036682792058	34.71607664642224	24.406697118744216
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.41431913165712	0.3561436122135675	0.17240588500338608	0.05713137112592644
12	99.51826180332777	0.2777313112290462	0.14417745664895842	0.05982942879421105
13	98.11567652447003	0.9719415492786743	0.28498234121256105	0.6273995850387306
14	60.45757034510856	22.66759659720967	8.727272236716788	8.147560820964987
15	33.05936806093294	24.88796315532448	20.282176361237543	21.77049242250504
16	31.07514900023473	17.716289792978035	27.985704341444595	23.22285686534264
17	23.151628142899927	19.24737006828784	28.943582265127333	28.6574195236849
18	21.553636037416663	20.238602729894748	33.35956070225045	24.848200530438138
19	20.542775006272983	21.31374498498531	30.6942169831938	27.44926302554791
20	25.170618421798146	18.753321983504076	26.14026662205878	29.935792972638996
21	29.075247479339623	18.004105094742297	22.697646214490188	30.223001211427892
22	29.089749539306652	23.01210483572876	21.0216802423935	26.876465382571084
23	28.91818679732461	21.169803608382328	20.459910910135793	29.452098684157274
24	26.007522184779177	21.76219589517506	21.80620796088896	30.424073959156807
25	30.057947533570584	19.965930276793735	22.728707603396316	27.247414586239366
26	30.95009402730974	20.855547341468878	20.415460410050805	27.77889822117058
27	28.526260195285413	23.248488413191343	22.45043668063361	25.774814710889633
28	31.2905551793264	22.91352455367381	19.8665405774383	25.929379689561483
29	29.203540121466553	22.40409754018082	19.580546464600136	28.811815873752483
30	28.167283622520145	25.212134784168878	20.12855570274959	26.492025890561383
31	27.715999751778696	23.27567134419931	22.447940977290447	26.560387926731543
32	27.83009386542628	25.93986838874694	19.781551760887336	26.448485984939442
33	28.29517155599684	27.01845066736456	20.777168766205207	23.90920901043339
34	27.87029492468372	25.53704837887205	19.240962181325663	27.35169451511857
35	27.390647722704426	26.313448198641797	20.697778419315934	25.598125659337846
36	30.809086788421013	26.733299697547736	19.74705034845415	22.710563165577103
37	29.037811929192177	25.49789281696107	20.22447165285711	25.23982360098965
38	26.958452609966088	27.653033830945102	21.662637567215363	23.725875991873448
39	29.61114918370265	26.325892989636763	22.093146393911024	21.969811432749566
40	28.68170204269946	27.92159174610198	19.239545701049813	24.157160510148746
41	30.18543075839703	27.74554348324641	18.600038582224656	23.468987176131904
42	30.50265488874559	28.253621467905255	18.093781786491906	23.149941856857247
43	30.021490029327886	27.931540833753782	17.42719291382134	24.61977622309699
44	27.03264919584391	27.28063442128012	19.500009443201836	26.186706939674124
45	25.94961512207362	28.07362729570982	20.397383423673297	25.579374158543267
46	25.94877197905228	26.102628717586207	21.778687772672452	26.169911530689056
47	27.247987923493877	23.850694884752464	22.863812841135665	26.03750435061799
48	26.405249610805182	25.70705973769483	21.29904057069316	26.588650080806826
49	28.072480621200796	24.341707654659412	20.57373521801655	27.01207650612324
50	26.236654732258245	27.93400281137609	20.56928342286388	25.26005903350178
51	27.122764321964617	25.43182412980895	21.097495662872298	26.347915885354134
52	28.87235354268462	24.790462096336846	20.1674751846146	26.169709176363938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	2.0
6	3.0
7	6.5
8	10.0
9	536.5
10	1063.0
11	1506.5
12	1950.0
13	3076.5
14	4546.5
15	4890.0
16	6127.5
17	7365.0
18	7402.0
19	7439.0
20	8934.0
21	10429.0
22	14194.5
23	17960.0
24	23755.0
25	29550.0
26	37241.5
27	44933.0
28	42814.5
29	40696.0
30	36211.0
31	31726.0
32	35035.0
33	38344.0
34	34894.0
35	31444.0
36	38342.0
37	45240.0
38	46342.5
39	50708.5
40	53972.0
41	59636.5
42	65301.0
43	69519.5
44	73738.0
45	80655.5
46	87573.0
47	100920.0
48	114267.0
49	123047.5
50	131828.0
51	153642.0
52	175456.0
53	191696.0
54	207936.0
55	234899.5
56	261863.0
57	285120.5
58	308378.0
59	301451.0
60	294524.0
61	271228.5
62	247933.0
63	226897.5
64	171248.0
65	136634.0
66	114509.5
67	92385.0
68	76055.5
69	59726.0
70	53394.0
71	47062.0
72	31457.0
73	15852.0
74	12127.0
75	8402.0
76	7366.0
77	6330.0
78	4056.0
79	1782.0
80	2243.5
81	2705.0
82	1633.0
83	561.0
84	421.0
85	281.0
86	164.0
87	47.0
88	27.0
89	3.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2965096.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.163885410928652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2130827431573	8.152765814587893
2	8.108477437497577	1.6482727106365151
3	2.997832733616499	0.9140888515682727
4	1.7072376198089663	0.6940867014785963
5	1.0434760299820935	0.5302885398894374
6	0.7686895512904035	0.4687723509536294
7	0.5541494300501758	0.3942617915292987
8	0.4358193478855786	0.35436943294197365
9	0.3591912515159439	0.32857008489144957
>10	2.4199645890230363	5.058671493220446
>50	0.28745796668391843	1.9952878119348987
>100	0.42578866217301176	11.739122978225073
>500	0.43860955691356124	32.4587383037775
>1k	0.24022308040188892	35.262703134365005
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAAGATCTCTGGGGCGAGTCCCGCTGGGCCGCAGCCGGTGGCCGGCTCGCCG	4266	0.1438739251612764	No Hit
GTCCATCTCTGGGGGCCGCCCATCCCTCTTTGTGTGCTTGGGACGCCGCGGA	3755	0.1266400818051085	No Hit
AATTATCTCTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATC	3662	0.12350358976572766	No Hit
GGGCATCTCTGGGATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG	3464	0.11682589703672326	No Hit
TATAATCTCTGGGTCTGACCCTGATCTCAGCCTATTCCTAAGCTTGGCCCTC	3396	0.11453254801868135	No Hit
AGCCATCTCTGGGGGGGCCGCCTCCGTCTCCTTGAGGAGATGCACCCAGGGG	3388	0.11426274225185289	No Hit
AGAAATCTCTGGGGGCGGACACCAGGACTTCAAGATGGCGTCACTCGTGCCG	3281	0.11065409012052224	No Hit
ACGGATCTCTGGGGCTCTCTTTCCCGCCTTGCAAGATGGCGGGTGAAAAAGC	3277	0.110519187237108	No Hit
TCCTATCTCTGGGATCGAGCCGCTGCCGCTCCCCTGGCATAGCCTGCTTCCT	3234	0.10906898124040502	No Hit
GTCGATCTCTGGGTTGATACCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	3223	0.10869799831101588	No Hit
CAGCATCTCTGGGGCTCTGACCCTGATCTCAGCCTATTCCTAAGCTTGGCCC	3078	0.10380776878725007	No Hit
CATCATCTCTGGGAGACGCTAGCTGTAGCTGGCAGGCGGTTGTACGTGCTCC	3078	0.10380776878725007	No Hit
CTCTATCTCTGGGTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCT	3006	0.10137951688579391	No Hit
AGATATCTCTGGGGCCTTTGCGGGCGCCTCTGGATCCCGCGGCCTCTTCTGG	2976	0.10036774526018719	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.005328663894862089	0.0	0.0	0.0	0.0
12	0.013422836899715894	0.0	0.0	0.0	0.0
13	0.016930311868485877	0.0	0.0	0.0	0.0
14	0.055917245175198374	0.0	0.0	0.0	0.0
15	0.09223984653447982	0.0	0.0	0.0	0.0
16	0.1171968799661124	0.0	0.0	0.0	0.0
17	0.13952330717116748	0.0	0.0	0.0	0.0
18	0.17486786262569576	0.0	0.0	0.0	0.0
19	0.2095379036631529	0.0	0.0	0.0	0.0
20	0.24032948680245092	0.0	0.0	0.0	0.0
21	0.2777313112290462	0.0	0.0	0.0	0.0
22	0.32194573126806014	0.0	0.0	0.0	0.0
23	0.3529396687324795	0.0	0.0	0.0	0.0
24	0.388722658558104	0.0	0.0	0.0	0.0
25	0.4234264253164147	0.0	0.0	0.0	0.0
26	0.4667977023340897	0.0	0.0	0.0	0.0
27	0.5111807509773714	0.0	0.0	0.0	3.372572085355752E-5
28	0.5555300738997996	0.0	0.0	0.0	3.372572085355752E-5
29	0.609153970056956	0.0	0.0	0.0	3.372572085355752E-5
30	0.6842611503978286	0.0	0.0	0.0	3.372572085355752E-5
31	0.7480027628110523	0.0	0.0	0.0	3.372572085355752E-5
32	0.7996705671587024	0.0	0.0	0.0	3.372572085355752E-5
33	0.839568094928461	0.0	0.0	0.0	3.372572085355752E-5
34	0.8814554402285795	0.0	0.0	0.0	3.372572085355752E-5
35	0.9252988773382043	0.0	0.0	0.0	3.372572085355752E-5
36	0.9704913432819713	0.0	0.0	0.0	3.372572085355752E-5
37	1.0150092948086673	0.0	0.0	0.0	3.372572085355752E-5
38	1.0594260691728026	0.0	0.0	0.0	3.372572085355752E-5
39	1.1090366045483857	0.0	0.0	0.0	3.372572085355752E-5
40	1.1615138261965212	0.0	0.0	0.0	3.372572085355752E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	35	1.02030754E-7	46.000004	36
CTAGATT	70	0.0	46.000004	46
ACAACCC	105	0.0	46.000004	28
GACAGTC	75	0.0	46.000004	40
CCTTACG	75	0.0	46.000004	39
CACGGTT	75	0.0	46.000004	38
CGAAAGT	35	1.02030754E-7	46.000004	44
TTAGCCT	75	0.0	46.000004	25
TAGACGA	70	0.0	46.000004	46
GCGAACC	115	0.0	46.000004	19
AACCAAT	35	1.02030754E-7	46.000004	31
ATAGCCG	105	0.0	46.000004	14
ACTTCTA	75	0.0	46.000004	27
TAAACCC	140	0.0	46.000004	26
ATATGTT	35	1.02030754E-7	46.000004	37
GTCTAGT	140	0.0	46.000004	32
AAGGTAC	75	0.0	46.000004	27
TACGGCA	75	0.0	46.000004	42
TAGCCGA	35	1.02030754E-7	46.000004	20
ATGTACC	75	0.0	46.000004	28
>>END_MODULE