FastQCFastQC Report
Fri 17 Jun 2016
SRR1528078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2725611
Sequences flagged as poor quality0
Sequence length59
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCATCTCTGGGGGCCGCCCATCCCTCTTTGTGTGCTTGGGACGCCGCGGATCTGCGG35280.12943886710172506No Hit
AATTATCTCTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGA32200.11813864854522528No Hit
TATAATCTCTGGGTCTGACCCTGATCTCAGCCTATTCCTAAGCTTGGCCCTCCCCAGCA30020.1101404419045858No Hit
GGGCATCTCTGGGATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGGTCGAGCT28090.10305946079612975No Hit
ACGGATCTCTGGGGCTCTCTTTCCCGCCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT27820.10206885722137166No Hit
AGAAATCTCTGGGGGCGGACACCAGGACTTCAAGATGGCGTCACTCGTGCCGCTGAAGG27480.1008214304976022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA650.053.00000431
ATAATCC1550.053.00000437
ATTCCCG650.053.00000416
CTTATGA1550.053.00000433
GTTCGAC650.053.00000415
TAACATA650.053.00000453
ACAACTA251.4792724E-553.019
ACAACGG150.00669724753.036
CTTAACG251.4792724E-553.025
CTAGACG700.053.045
ACACGTG307.0140595E-753.045
CATATAG251.4792724E-553.048
CTCCGTA457.6397555E-1153.047
ACGTTCA307.0140595E-753.039
ACGTTAA850.053.052
TTAGCAC251.4792724E-553.046
TAGACGG150.00669724753.052
TAGACGA700.053.046
GACACGT251.4792724E-553.044
TAGGTTA503.6379788E-1253.033