FastQCFastQC Report
Fri 17 Jun 2016
SRR1528077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176606
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTAAGGGGGGAGACGCTAGCTGTAGCTGGCAGGCGGTTGTACGTGCTCC33060.28097765947139486No Hit
GCTATAAGGGGGGGCTCCCTGAAGAAACTCAGCCAATTGATTTAATACTTGC32220.27383848119081494No Hit
GCTCTAAGGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG28960.24613167024475482No Hit
AATCTAAGGGGAGGGGCTGTCTCGGAGGGAGGGCACTGAGGACTGATGTTTC28500.24222212023396109No Hit
TGCATAAGGGGGTATGTTTTCGGCATCGGTTACCGACGCCAGCTTTTGCTTC22600.19207789183464985No Hit
GACGTAAGGGGGGAGCCACCTCCCACCTAGCTCCCCACAGGCACCCACCTTC21210.1802642515846426No Hit
TTAGTAAGGGGGGGTTTCACTGTGTTAGCCAGGAGATCGAGTCCATCCTGGC19930.16938550372852085No Hit
TTTATAAGGGGGGACGCTCTCGGTGGCCGTAGGTGGTCGCGGCCACCGCAGC19780.1681106504641316No Hit
CTGGTAAGGGGGGGGCGCATGTCTCCTCTCTCCAAGACTATGAAACCGTCCG19770.16802566024650564No Hit
ACGTTAAGGGGGGGTCGGGCAAATGCCGTTGACGTGGTGCTAATGGACATGA19710.16751571894074993No Hit
GCCGTAAGGGGGATTTATCCTCTCCATGACGTCTTTGTTTAAAAAAGTAAAA18970.1612264428364295No Hit
ATGTTAAGGGGGGGCTCATACCTGTAATCCCAGCACTTTGGGAGGCTGAGGT18730.15918667761340669No Hit
TCACTAAGGGGGGAATCCACTGGCCCTCACTATTCAAGTCAAAATTGCACAC18600.15808180478426934No Hit
GGATTAAGGGGGGGTTAACAGAGAACCTACAGAATGGCAGAAAGTATTTGCA18370.15612702977887244No Hit
TGTTTAAGGGGGGTGTGCTTCTGGCTGGGCGCAGTGTTCACGCCTGTAATCC17790.15119759715656728No Hit
AGCTTAAGGGGGTAGCCGAGGGGCAGGCAGGAGCTCGGTCTGTACAGAGCTC17620.14975276345692612No Hit
AGTATAAGGGGGGATATCAACGGCTGGAACTCACCTTAGGCAAGGCCACCTC17320.14720305692814759No Hit
CTTATAAGGGGGAGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGC16850.14320851669972787No Hit
GGTGTAAGGGGGCGGTCCTTCGGAGCGGAAGGAATATGGCGTCCGCTACTCG16730.14218863408821644No Hit
TTAGTAAGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCC16630.14133873191195695No Hit
GCTGTAAGGGGGACCGAGGTGGAAGCTGCCGTGAACCGCCTGGTCAACTTGC16430.1396389275594379No Hit
CTTCTAAGGGGGGTCCCCATTTTTACATACGTGGAAACTGAAGCACAAAGAG16300.13853405473030056No Hit
ATAGTAAGGGGGGTCACAGAAAATCAACGAGGAAATTGGCTTTTTCTGTGGC16200.137684152554041No Hit
GCTCTAAGGGGGGACCCTCGCCAGTCCTGGGCTCTCTAGGCCTCACAGATCC15760.13394458297849918No Hit
GTTCTAAGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCCA15640.13292470036698775No Hit
TTAGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG15560.13224477862598014No Hit
TAGCTAAGGGGGGCACCCATGAGTAGGTTGGGGTAGCTGTGCACTCTCAGTG15400.13088493514396493No Hit
GGTTTAAGGGGGCAGAGGGAGTCAGATCTCGTTATGGATGGTTGTGAGCCAC15260.1296950720972016No Hit
TTATTAAGGGGGGACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAG15090.12825023839756045No Hit
TAAGTAAGGGGGGAGGCTGAGCCACAAACTTCTGCAAGGAGGACATGATGGA15060.1279952677446826No Hit
TCTTTAAGGGGGGGAACAAAGATGGCGGCTGCCGCTGCTAGCCGAGCGGTCG15050.12791027752705664No Hit
TACTTAAGGGGGGGAGTGTATGGCCAGCCATGGAGCCAGCAGGGGTTCAATC15040.12782528730943069No Hit
GATTTAAGGGGGGTGAGCCACCGTGCCTGGCCTACAAAAACTTTTTTAAAAA14930.1268903949155452No Hit
CGGTTAAGGGGGGGGGCCAACCCCGACCGTCAGCTCTGCTGTGCTCGGTGGA14840.12612548295691167No Hit
GGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14820.12595550252165977No Hit
GCGCTAAGGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG14760.12544556121590406No Hit
ATCCTAAGGGGGGGACAGGCGACAGCGCTGCAGACGCCATTATCCTCTGCTT14470.12298084490475146No Hit
TTCCTAAGGGGGAGGAGGCCGAGGTGGGCGGATCATGAGGTCAGGAGATCGA14450.12281086446949957No Hit
GTCTTAAGGGGGTGGGCCCCCACTGGCAGAGCACACTGGAGAAAAAGCTCAC14350.12196096229324005No Hit
AGACTAAGGGGGGCTACTCGGCTGCGCCCACCTTTCCTACTCCCAACACTGA14310.12162100142273624No Hit
AGGTTAAGGGGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCT14290.12145102098748434No Hit
GTTTTAAGGGGGGACCCACGGCTCGCGCGCCCGGCTTAAGGAGGTTCCCAAG14170.12043113837597293No Hit
ACTATAAGGGGGGCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTAC14160.12034614815834697No Hit
GGTCTAAGGGGGGGAATGTTACAGAGCGGAGAGAGTGAGGAGGCTGCGTCTG14110.11992119707021723No Hit
ATGTTAAGGGGGGACATTAAATGCACCAAAGGGGTCCCATAAGGAGAAGAGA13900.11813640250007225No Hit
TTGCTAAGGGGGGAGAAGCAGGAGGGATCCTCACCTGCGGTGTGCCCGCTTC13810.1173714905414387No Hit
GGTGTAAGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTA13730.11669156880043108No Hit
AATATAAGGGGGGATACGATCAGGGGCTGGTGAAGGGGCTGGAGGCGCCATG13550.11516174488316396No Hit
GTGCTAAGGGGGGAGTCCTGTAAGGGGGGTCCCGATCCAGGGACCGAACCCA13450.11431184270690445No Hit
GTCGTAAGGGGGGAGACTGGCACCTGAAGATGCCCAAGGTGAAAATGCCCAA13410.11397188183640064No Hit
GTTCTAAGGGGGGGAGTCCATTAAGGGGGGTGGCTCAGACCCTGGAGGCTAA13390.11380190140114874No Hit
GATTTAAGGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG13220.11235706770150755No Hit
TCGGTAAGGGGGGTTGTCTTCCTGCTTATGGATACCATATGCTTCTATATAT13120.11150716552524803No Hit
GTTTTAAGGGGGGAACCCAGGATGGCCCATCTGCTGCCCACCAAGTCCCTTT13120.11150716552524803No Hit
TTTCTAAGGGGGAGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCA12960.11014732204323281No Hit
TATATAAGGGGGGATGGGCCGCCGCCCCGCCCGGTGTTACCGGTATTGTAAG12880.10946740030222521No Hit
GTGTTAAGGGGGCATTCTATCTTTGCTCAAATTGTTGAAGGATGGTGATTTG12790.10870248834359164No Hit
CCTTTAAGGGGGGGGCCTACCTCGAGTTTGTGCGTCTGTAGTGTCGGAGTCG12740.1082775372554619No Hit
GAACTAAGGGGGGCTTTCTCTGCGGAATCACCATGGCGGCTGGGACCCTGTA12730.10819254703783594No Hit
GCGGTAAGGGGGGCATGAAAATGAAGGATTAGAGAGATGGCTCAGCAGTTAA12540.10657773290294287No Hit
ACGCTAAGGGGGGGGCTCCCGGAAGTGGAGGGTTTACACTGTGTGCCGCTGG12520.10640775246769096No Hit
GCCTTAAGGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAA12510.10632276225006501No Hit
CTATTAAGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGC12420.10555785029143146No Hit
TGGATAAGGGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGC12410.1054728600738055No Hit
GATTTAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGAACTGACTG12220.10385804593891243No Hit
CTCCTAAGGGGGGGAGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGT12050.10241321223927126No Hit
GTTTTAAGGGGTGACACAGAAGATGATGGTCTTGCCTTCCCACAGCTTCTAG12030.10224323180401935No Hit
CCGTTAAGGGGGGGTTCGTCCCTTTCCTGTTCGGCGCGTGCGGTTGAGTCGG12020.1021582415863934No Hit
GAATTAAGGGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG12000.1019882611511415No Hit
CAATTAAGGGGGGACCACCTTATCGAGCACTCGAGGCTGCACTCGGGCGAGA11990.10190327093351555No Hit
TTCATAAGGGGGGGCCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAT11980.1018182807158896No Hit
TCAATAAGGGGGGGCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCC11980.1018182807158896No Hit
GTGGTAAGGGGGGGTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGA11980.1018182807158896No Hit
TACGTAAGGGGGGAGTCGTTTCTTTCCTCAACTTGCTTTTGGTCATGGTGTT11870.10088338832200414No Hit
CTGCTAAGGGGGTGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG11840.10062841766912628No Hit
TGTTTAAGGGGGGAGTCCAGTGACTCGGCAGGAGAAGATGGCGACCGTGTGG11830.10054342745150033No Hit
CCAGTAAGGGGGGGACTCCAGAGAGTGCTCTTAACCTGAGCTAATCATTATA11770.10003348614574463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTG405.6115823E-946.00000415
CCTTCGG1600.046.00000417
TTTAGTC405.6115823E-946.00000445
TTCTAGT405.6115823E-946.00000420
AACGTCA206.311515E-446.00000419
TAATACC206.311515E-446.00000421
AGGTAAC405.6115823E-946.00000424
ACTATCT551.8189894E-1246.00000441
CTCACTA2200.046.00000426
GTACCTT405.6115823E-946.00000439
TGATACG206.311515E-446.00000444
GATACCT405.6115823E-946.00000413
CTCACAT405.6115823E-946.00000443
TCACCTC551.8189894E-1246.00000424
ACGCCCA405.6115823E-946.00000437
CCCTCAC2200.046.00000424
ATAGGTA551.8189894E-1246.00000434
CAAGTAG206.311515E-446.00000425
GTACAAG206.311515E-446.00000441
TGGACGT405.6115823E-946.00000422
Produced by FastQC (version 0.11.5)