FastQCFastQC Report
Fri 17 Jun 2016
SRR1528076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168232
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTAAGGGGGGAGACGCTAGCTGTAGCTGGCAGGCGGTTGTACGTGCTCC32300.27648617740311854No Hit
GCTATAAGGGGGGGCTCCCTGAAGAAACTCAGCCAATTGATTTAATACTTGC31870.2728054016667922No Hit
GCTCTAAGGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG29720.2544015229851605No Hit
AATCTAAGGGGAGGGGCTGTCTCGGAGGGAGGGCACTGAGGACTGATGTTTC27410.23462805333187242No Hit
TGCATAAGGGGGTATGTTTTCGGCATCGGTTACCGACGCCAGCTTTTGCTTC21360.18284039471611804No Hit
ACGTTAAGGGGGGGTCGGGCAAATGCCGTTGACGTGGTGCTAATGGACATGA20330.1740236528360805No Hit
TTAGTAAGGGGGGGTTTCACTGTGTTAGCCAGGAGATCGAGTCCATCCTGGC19810.16957248217819748No Hit
GACGTAAGGGGGGAGCCACCTCCCACCTAGCTCCCCACAGGCACCCACCTTC19590.16768929459217005No Hit
TTTATAAGGGGGGACGCTCTCGGTGGCCGTAGGTGGTCGCGGCCACCGCAGC19350.16563490813468557No Hit
CTGGTAAGGGGGGGGCGCATGTCTCCTCTCTCCAAGACTATGAAACCGTCCG19170.16409411829157222No Hit
GCCGTAAGGGGGATTTATCCTCTCCATGACGTCTTTGTTTAAAAAAGTAAAA18120.15510617754007766No Hit
GGATTAAGGGGGGGTTAACAGAGAACCTACAGAATGGCAGAAAGTATTTGCA18030.154335782618521No Hit
ATGTTAAGGGGGGGCTCATACCTGTAATCCCAGCACTTTGGGAGGCTGAGGT17860.15288059221113615No Hit
AGCTTAAGGGGGTAGCCGAGGGGCAGGCAGGAGCTCGGTCTGTACAGAGCTC17690.1514254018037513No Hit
TCACTAAGGGGGGAATCCACTGGCCCTCACTATTCAAGTCAAAATTGCACAC17670.15125420293229427No Hit
TGTTTAAGGGGGGTGTGCTTCTGGCTGGGCGCAGTGTTCACGCCTGTAATCC17620.1508262057536517No Hit
AGTATAAGGGGGGATATCAACGGCTGGAACTCACCTTAGGCAAGGCCACCTC17450.14937101534626684No Hit
GGTGTAAGGGGGCGGTCCTTCGGAGCGGAAGGAATATGGCGTCCGCTACTCG17090.14628943566004013No Hit
ATAGTAAGGGGGGTCACAGAAAATCAACGAGGAAATTGGCTTTTTCTGTGGC16790.14372145258818456No Hit
CTTCTAAGGGGGGTCCCCATTTTTACATACGTGGAAACTGAAGCACAAAGAG16370.14012627628758673No Hit
CTTATAAGGGGGAGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGC16300.13952708023748706No Hit
TAGCTAAGGGGGGCACCCATGAGTAGGTTGGGGTAGCTGTGCACTCTCAGTG15900.13610310280834628No Hit
GCTGTAAGGGGGACCGAGGTGGAAGCTGCCGTGAACCGCCTGGTCAACTTGC15770.13499031014387552No Hit
GTTCTAAGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCCA15510.132764724814934No Hit
GCTCTAAGGGGGGACCCTCGCCAGTCCTGGGCTCTCTAGGCCTCACAGATCC15420.13199432989337734No Hit
TTAGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG15350.1313951338432777No Hit
TTAGTAAGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCC15050.1288271507714221No Hit
TAAGTAAGGGGGGAGGCTGAGCCACAAACTTCTGCAAGGAGGACATGATGGA15000.1283991535927795No Hit
GATTTAAGGGGGGTGAGCCACCGTGCCTGGCCTACAAAAACTTTTTTAAAAA14950.12797115641413692No Hit
GCGCTAAGGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG14920.12771435810695136No Hit
GTCTTAAGGGGGTGGGCCCCCACTGGCAGAGCACACTGGAGAAAAAGCTCAC14740.126173568263838No Hit
TCTTTAAGGGGGGGAACAAAGATGGCGGCTGCCGCTGCTAGCCGAGCGGTCG14600.12497517616363873No Hit
TTATTAAGGGGGGACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAG14590.1248895767279102No Hit
AGACTAAGGGGGGCTACTCGGCTGCGCCCACCTTTCCTACTCCCAACACTGA14590.1248895767279102No Hit
ATCCTAAGGGGGGGACAGGCGACAGCGCTGCAGACGCCATTATCCTCTGCTT14580.12480397729218169No Hit
CGGTTAAGGGGGGGGGCCAACCCCGACCGTCAGCTCTGCTGTGCTCGGTGGA14350.12283519027042573No Hit
GGTGTAAGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTA14280.1222359942203261No Hit
GGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14280.1222359942203261No Hit
AGGTTAAGGGGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCT14160.12120880099158386No Hit
GGTTTAAGGGGGCAGAGGGAGTCAGATCTCGTTATGGATGGTTGTGAGCCAC14120.12086640324866979No Hit
TACTTAAGGGGGGGAGTGTATGGCCAGCCATGGAGCCAGCAGGGGTTCAATC14010.11992480945565608No Hit
GTTTTAAGGGGGGACCCACGGCTCGCGCGCCCGGCTTAAGGAGGTTCCCAAG13930.11924001396982792No Hit
GGTCTAAGGGGGGGAATGTTACAGAGCGGAGAGAGTGAGGAGGCTGCGTCTG13900.11898321566264235No Hit
TTCCTAAGGGGGAGGAGGCCGAGGTGGGCGGATCATGAGGTCAGGAGATCGA13860.11864081791972828No Hit
ATGTTAAGGGGGGACATTAAATGCACCAAAGGGGTCCCATAAGGAGAAGAGA13650.11684322976942936No Hit
GTTCTAAGGGGGGGAGTCCATTAAGGGGGGTGGCTCAGACCCTGGAGGCTAA13580.11624403371932972No Hit
GATTTAAGGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG13500.11555923823350156No Hit
GTGCTAAGGGGGGAGTCCTGTAAGGGGGGTCCCGATCCAGGGACCGAACCCA13360.1143608461333023No Hit
TTGCTAAGGGGGGAGAAGCAGGAGGGATCCTCACCTGCGGTGTGCCCGCTTC13300.11384724951893116No Hit
ACTATAAGGGGGGCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTAC13280.11367605064747414No Hit
TATATAAGGGGGGATGGGCCGCCGCCCCGCCCGGTGTTACCGGTATTGTAAG13240.11333365290456004No Hit
GCCTTAAGGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAA13160.11264885741873189No Hit
GTTTTAAGGGGGGAACCCAGGATGGCCCATCTGCTGCCCACCAAGTCCCTTT13000.11127926644707559No Hit
TTTCTAAGGGGGAGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCA12930.11068007039697594No Hit
TCGGTAAGGGGGGTTGTCTTCCTGCTTATGGATACCATATGCTTCTATATAT12820.10973847660396222No Hit
CCTTTAAGGGGGGGGCCTACCTCGAGTTTGTGCGTCTGTAGTGTCGGAGTCG12790.10948167829677667No Hit
GAACTAAGGGGGGCTTTCTCTGCGGAATCACCATGGCGGCTGGGACCCTGTA12710.10879688281094851No Hit
CTGCTAAGGGGGTGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG12530.10725609296783516No Hit
CCGTTAAGGGGGGGTTCGTCCCTTTCCTGTTCGGCGCGTGCGGTTGAGTCGG12470.10674249635346403No Hit
GTGTTAAGGGGGCATTCTATCTTTGCTCAAATTGTTGAAGGATGGTGATTTG12310.10537290538180771No Hit
GTGGTAAGGGGGGGTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGA12270.10503050763889366No Hit
TTCATAAGGGGGGGCCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAT12250.1048593087674366No Hit
GATTTAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGAACTGACTG12230.10468810989597956No Hit
CTCTTAAGGGGGGGTATCAACGCAGAGTGGTTTAAGGGGGGGGTCCTCCACT12200.10443131158879401No Hit
GAATTAAGGGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG12140.10391771497442288No Hit
AATATAAGGGGGGATACGATCAGGGGCTGGTGAAGGGGCTGGAGGCGCCATG12130.10383211553869436No Hit
TGGATAAGGGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGC12130.10383211553869436No Hit
TTGGTAAGGGGGGGCTTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAA12070.10331851892432324No Hit
ACGCTAAGGGGGGGGCTCCCGGAAGTGGAGGGTTTACACTGTGTGCCGCTGG12050.1031473200528662No Hit
CTATTAAGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGC12040.1030617206171377No Hit
TCAATAAGGGGGGGCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCC12030.10297612118140918No Hit
GCGGTAAGGGGGGCATGAAAATGAAGGATTAGAGAGATGGCTCAGCAGTTAA11960.10237692513130954No Hit
TACGTAAGGGGGGAGTCGTTTCTTTCCTCAACTTGCTTTTGGTCATGGTGTT11950.10229132569558103No Hit
TGTTTAAGGGGGGAGTCCAGTGACTCGGCAGGAGAAGATGGCGACCGTGTGG11920.10203452738839545No Hit
GTCGTAAGGGGGGAGACTGGCACCTGAAGATGCCCAAGGTGAAAATGCCCAA11810.10109293359538173No Hit
GCAGTAAGGGGCCAGCTATTTTAAAACAAAAAATTTTGTAGAGACAGGACCT11780.10083613528819618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTG351.0195981E-746.00000443
CCTTCAA700.046.00000439
CTTGTCC700.046.00000432
AGACTTG351.0195981E-746.00000436
AGCGTCC700.046.00000419
CTATGCA351.0195981E-746.00000446
CCTTATA351.0195981E-746.00000432
TACCCTA700.046.00000423
GCAAGCC700.046.00000446
ACTTGTC700.046.00000431
ACTTGTA700.046.00000443
TAGACGT351.0195981E-746.00000442
TAGGTCC351.0195981E-746.00000440
TAGCGCA700.046.00000428
CCAATTG3300.046.00000433
ATAGCCT351.0195981E-746.00000441
CCAATCT351.0195981E-746.00000429
AGGACAT1650.046.00000440
GCACGAC351.0195981E-746.00000412
CCTACCA351.0195981E-746.00000434
Produced by FastQC (version 0.11.5)