SRR1528075_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528075_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1071449
Sequences flagged as poor quality	0
Sequence length	59
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCGGTAAGGGGGGAGACGCTAGCTGTAGCTGGCAGGCGGTTGTACGTGCTCCAGAGTCG	3004	0.2803679876503688	No Hit
GCTATAAGGGGGGGCTCCCTGAAGAAACTCAGCCAATTGATTTAATACTTGCTTAGTGC	2724	0.25423515258309076	No Hit
GCTCTAAGGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG	2526	0.23575550492837272	No Hit
AATCTAAGGGGAGGGGCTGTCTCGGAGGGAGGGCACTGAGGACTGATGTTTCTGCACAC	2060	0.1922630008521171	No Hit
GACGTAAGGGGGGAGCCACCTCCCACCTAGCTCCCCACAGGCACCCACCTTCCCTGCAT	1860	0.17359669008977563	No Hit
GGATTAAGGGGGGGTTAACAGAGAACCTACAGAATGGCAGAAAGTATTTGCAAACTATG	1799	0.16790346530726147	No Hit
GCCGTAAGGGGGATTTATCCTCTCCATGACGTCTTTGTTTAAAAAAGTAAAAATGCTGA	1675	0.15633035263460976	No Hit
TGCATAAGGGGGTATGTTTTCGGCATCGGTTACCGACGCCAGCTTTTGCTTCGCGCAGT	1671	0.15595702641936293	No Hit
ACGTTAAGGGGGGGTCGGGCAAATGCCGTTGACGTGGTGCTAATGGACATGAGTATGCC	1668	0.1556770317579278	No Hit
TCACTAAGGGGGGAATCCACTGGCCCTCACTATTCAAGTCAAAATTGCACACTATTAAC	1607	0.1499838069754137	No Hit
ATGTTAAGGGGGGGCTCATACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGCAGA	1576	0.14709052880725074	No Hit
TTAGTAAGGGGGGGTTTCACTGTGTTAGCCAGGAGATCGAGTCCATCCTGGCTAACACA	1549	0.14457057685433464	No Hit
TTTATAAGGGGGGACGCTCTCGGTGGCCGTAGGTGGTCGCGGCCACCGCAGCCGGGCGG	1497	0.13971733605612585	No Hit
TAAGTAAGGGGGGAGGCTGAGCCACAAACTTCTGCAAGGAGGACATGATGGAGGACATG	1489	0.1389706836256322	No Hit
CTTATAAGGGGGAGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTG	1485	0.1385973574103854	No Hit
CTGGTAAGGGGGGGGCGCATGTCTCCTCTCTCCAAGACTATGAAACCGTCCGCAAAGGA	1476	0.13775737342608002	No Hit
ATAGTAAGGGGGGTCACAGAAAATCAACGAGGAAATTGGCTTTTTCTGTGGCCGTTGTC	1410	0.13159749087450734	No Hit
AGTATAAGGGGGGATATCAACGGCTGGAACTCACCTTAGGCAAGGCCACCTCTGTTGAA	1332	0.12431762967719416	No Hit
CTTCTAAGGGGGGTCCCCATTTTTACATACGTGGAAACTGAAGCACAAAGAGATCTAGT	1316	0.12282432481620684	No Hit
GCTGTAAGGGGGACCGAGGTGGAAGCTGCCGTGAACCGCCTGGTCAACTTGCACCTGCG	1306	0.12189100927808977	No Hit
GTTCTAAGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCCAAGTGCAG	1298	0.12114435684759611	No Hit
GCGCTAAGGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG	1287	0.12011770975566734	No Hit
GATTTAAGGGGGGTGAGCCACCGTGCCTGGCCTACAAAAACTTTTTTAAAAAATAGCTG	1285	0.1199310466480439	No Hit
GCTCTAAGGGGGGACCCTCGCCAGTCCTGGGCTCTCTAGGCCTCACAGATCCTTTGTTT	1283	0.11974438354042048	No Hit
GGTGTAAGGGGGCGGTCCTTCGGAGCGGAAGGAATATGGCGTCCGCTACTCGCGTTATC	1261	0.11769108935656294	No Hit
TGTTTAAGGGGGGTGTGCTTCTGGCTGGGCGCAGTGTTCACGCCTGTAATCCCAGCACT	1248	0.11647777915701073	No Hit
GTGCTAAGGGGGGAGTCCTGTAAGGGGGGTCCCGATCCAGGGACCGAACCCAGGTCTCC	1244	0.1161044529417639	No Hit
TTAGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1232	0.11498447429602343	No Hit
TTGCTAAGGGGGGAGAAGCAGGAGGGATCCTCACCTGCGGTGTGCCCGCTTCACTTCTG	1227	0.11451781652696488	No Hit
AGCTTAAGGGGGTAGCCGAGGGGCAGGCAGGAGCTCGGTCTGTACAGAGCTCACCGAGC	1207	0.11265118545073073	No Hit
ATCCTAAGGGGGGGACAGGCGACAGCGCTGCAGACGCCATTATCCTCTGCTTCTCCGCT	1199	0.11190453302023708	No Hit
TTATTAAGGGGGGACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCC	1175	0.1096645757287561	No Hit
CGGTTAAGGGGGGGGGCCAACCCCGACCGTCAGCTCTGCTGTGCTCGGTGGATGCTGGG	1169	0.10910458640588587	No Hit
TAGCTAAGGGGGGCACCCATGAGTAGGTTGGGGTAGCTGTGCACTCTCAGTGAGAGATG	1164	0.10863792863682732	No Hit
TCGGTAAGGGGGGTTGTCTTCCTGCTTATGGATACCATATGCTTCTATATATCCATTGT	1161	0.10835793397539219	No Hit
AGACTAAGGGGGGCTACTCGGCTGCGCCCACCTTTCCTACTCCCAACACTGACATTTTT	1154	0.10770461309871025	No Hit
ATGTTAAGGGGGGACATTAAATGCACCAAAGGGGTCCCATAAGGAGAAGAGAGAGAGAG	1144	0.10677129756059317	No Hit
CTGCTAAGGGGGTGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCT	1138	0.10621130823772293	No Hit
GGTTTAAGGGGGCAGAGGGAGTCAGATCTCGTTATGGATGGTTGTGAGCCACCATATGG	1135	0.10593131357628781	No Hit
ACTATAAGGGGGGCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTC	1132	0.10565131891485269	No Hit
AGGTTAAGGGGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCA	1131	0.10555798736104098	No Hit
GTCTTAAGGGGGTGGGCCCCCACTGGCAGAGCACACTGGAGAAAAAGCTCACAGGCTGA	1124	0.10490466648435902	No Hit
GTGTTAAGGGGGCATTCTATCTTTGCTCAAATTGTTGAAGGATGGTGATTTGTTTCCTG	1111	0.10369135628480683	No Hit
TCTTTAAGGGGGGGAACAAAGATGGCGGCTGCCGCTGCTAGCCGAGCGGTCGGCGCAAA	1109	0.10350469317718342	No Hit
GGTCTAAGGGGGGGAATGTTACAGAGCGGAGAGAGTGAGGAGGCTGCGTCTGGCTCCCG	1107	0.10331803006956	No Hit
TTAGTAAGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACA	1093	0.1020113883161961	No Hit
GGTGTAAGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG	1079	0.1007047465628322	No Hit
GCGGTAAGGGGGGCATGAAAATGAAGGATTAGAGAGATGGCTCAGCAGTTAAGAGCACT	1072	0.10005142568615025	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTGAT	75	0.0	53.000004	29
GTTGACG	150	0.0	53.000004	28
TCGCACA	65	0.0	53.000004	30
CGAACGC	90	0.0	53.000004	38
ACACCTA	45	7.6397555E-11	53.000004	21
TAGAATT	45	7.6397555E-11	53.000004	38
CTATAGG	130	0.0	53.000004	24
TTAGAAT	75	0.0	53.000004	44
CCAATTG	300	0.0	53.000004	33
TTGTCGA	75	0.0	53.000004	26
ATAGCCT	75	0.0	53.000004	41
TCCCTAG	45	7.6397555E-11	53.000004	39
AGGACGC	90	0.0	53.000004	21
ACTTCGA	45	7.6397555E-11	53.000004	40
TATATGG	65	0.0	53.000004	17
TTCGTGC	90	0.0	53.000004	15
GTAGGTC	45	7.6397555E-11	53.000004	39
CAATTAG	75	0.0	53.000004	41
CTAAGGC	45	7.6397555E-11	53.000004	29
TAACGGG	45	7.6397555E-11	53.000004	42

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content