FastQCFastQC Report
Fri 17 Jun 2016
SRR1528074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325629
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGCAGGAGGGATAAACCCTTGGTTTGTACCTCGAATCTTGCGCCTGTTT50490.38087579556572765No Hit
GCAAGCAGGAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGC48210.36367641323477384No Hit
CATTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG47220.3562082603805439No Hit
CCCGGCAGGAGGGGTTGCAACCCGCGGAGGACGAGATTCTCAGGTGCTGGGG34030.2567083248782276No Hit
CTTGGCAGGAGGGGGGAGGCAGAGGCGAGTGCAGCACGAGGTCAAGAGATTG32560.24561924942800736No Hit
CAGAGCAGGAGGGGGCTAGCTGGTGCGCTCTGTGACTTGATTGCTGCTGCTT25990.19605787139539038No Hit
AATTGCAGGAGGGGGTGGCCCACAGGAGAGCACAGGCCCAGACTGAGGGACT25100.1893440774153251No Hit
CTTTGCAGGAGGGAGCCTGGGATAAATGAGACCCCGTCTCAAAATTTAAAGA24990.18851428265374398No Hit
CCTTGCAGGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG24880.1876844878921629No Hit
CTTTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG24600.1855722830445019No Hit
ATGAGCAGGAGGGCAAGCTGTGCCGCTGCCCACCGAAAGGGCAGCAGCAGCA24530.18504423183258664No Hit
GTCGGCAGGAGGGGCCAGGCTGGTCGCGAACTCCTGACCTCAAGTGATCCAC24370.18383725763392322No Hit
TTTTGCAGGAGGGGTGGCCCTGGTGCTCACACATGTAATCCCAGCACTTTGG23840.17983915560085062No Hit
CCTGGCAGGAGGGGCCTGCGGACTAGCGGTGGCTTCGCGTGGGTGGAGGAGG23340.1760673612300274No Hit
CCTTGCAGGAGGGAAGCAGTGGTATCAACGGGGGCCGGTAGTGGTGGCGCAC23030.173728848720117No Hit
GCTTGCAGGAGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCT22300.16822202893871513No Hit
GCTTGCAGGAGGGATTAGTCCTTTCACAATTAAAAATGTGTTATGAGACTTC22100.16671331119038585No Hit
GTTAGCAGGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCC22020.16610982409105413No Hit
GCAGGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG21890.16512915755464008No Hit
AGTCGCAGGAGGGGGCTTTTAAAGGCGCTAGCTGGTGCGCTCTGTGACTTGA21870.16497828577980717No Hit
TCTGGCAGGAGGGGCTTCCACCTTCCCCCACCCTCCTCATCCAACCTCTGTT21860.1649028498923907No Hit
CTTGGCAGGAGGGGGGAGGACGGACCGCAGAGGTTGTCTGAAGGCCGAGGCC21750.16407305513080958No Hit
GTAAGCAGGAGGGGGTGCCCCCGACCTACAACTTGCGCCAATTATCGAGCTG20840.15720838937591136No Hit
GAGTGCAGGAGGGCAAGTACTGGGATACAGCCACCCTTTCAACCCAGCACTC20550.1550207486408339No Hit
GTTGGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC20220.15253136435609058No Hit
GCTAGCAGGAGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAG20040.1511735183825942No Hit
CTTTGCAGGAGGGGTCTAGCAGTGACCGACCGTCGGAGGCGGCGCGGCCGGG19870.14989110829651434No Hit
GTTTGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC19350.1459684421508582No Hit
GGTAGCAGGAGGGAGTGAACGCAGGAGGGGGCACTGCTGCTGCTGCCACTGG19330.14581757037602527No Hit
TGAAGCAGGAGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCT19220.14498777561444415No Hit
TCCTGCAGGAGGGGCAGAGCAGAGAGGAGCAGCGGGCTTGTCGTTGGTGGCG18990.1432527502038655No Hit
TATCGCAGGAGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC18990.1432527502038655No Hit
ATATGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG18860.14227208366745145No Hit
TATTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG18210.1373687509853813No Hit
AGACGCAGGAGGGAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACAC18100.13653895622380016No Hit
ATTTGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC17960.1354828537999697No Hit
TCTTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG17900.13503023847547088No Hit
CATAGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG17880.13487936670063796No Hit
GCCCGCAGGAGGGGCTTTTCCTCTTCCGGGGACGGTGCTCAGAGGCACTCAG17600.13276716185297696No Hit
GCGTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG17480.1318619312039794No Hit
CCGGGCAGGAGGGGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACT17290.13042864934306655No Hit
ACCAGCAGGAGGGGGAGAAAGCCAGAGCCGGTTTGCAGGCAACGCTGTGGGG17160.12944798280665254No Hit
GCTGGCAGGAGGGGGCTCCTGGTGCTGCTTGTGCGCTCCCTCAGTGCGGACC17150.12937254691923605No Hit
CACTGCAGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG17120.12914623925698668No Hit
CGTAGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG17110.12907080336957022No Hit
TGGGGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG17050.1286181880450714No Hit
ACAGGCAGGAGGGGACCAGCTTGGTCAACATGGTGAAACTCTGTCTCTACTA17030.1284673162702385No Hit
TCTTGCAGGAGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC16830.12695859852190922No Hit
GCACGCAGGAGGGGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTT16770.12650598319741044No Hit
GGAAGCAGGAGGGAGATCAGGAATTAGAGACCAGCCTGGCCAATACAATATG16750.1263551114225775No Hit
GGCAGCAGGAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGC16680.12582706021066226No Hit
CCTTGCAGGAGGGGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACT16580.12507270133649762No Hit
TTCTGCAGGAGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC16560.12492182956166469No Hit
CATCGCAGGAGGGGGAGACTGAGTACACCTTCCCACTTACTGAAAAGGTCAA16500.1244692142371659No Hit
GGAGGCAGGAGGGAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAG16440.12401659891266711No Hit
TTGTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG16440.12401659891266711No Hit
AATCGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG16310.12303593237625307No Hit
CTTTGCAGGAGGGGAAGGTGCTCGGTCCTTCCGAGGAAGCTAAGGCCGCGTT16260.12265875293917076No Hit
CGTGGCAGGAGGGGGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGC16130.12167808640275674No Hit
GATGGCAGGAGGGGCTCTGTTGAGGTTTTTTCTTCAGCAAGTACACCATGTC16030.1209237275285921No Hit
CATGGCAGGAGGGATGTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGA15960.12039567631667684No Hit
TTGGGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC15930.12016936865442746No Hit
ATGAGCAGGAGGGGCGGGTAGATCACTTGAGGCCAGTTCAAGACCAGCCTGA15920.12009393276701098No Hit
TTGGGCAGGAGGGGGTCTCTCATGAGACTCTGAAGGGTGGAAGGAAGAAGAC15850.11956588155509573No Hit
CCATGCAGGAGGGGCAGAGTCGCGCAGGAGGGGCTTTTTTTCCGACATCTTA15820.11933957389284634No Hit
AGTTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG15810.11926413800542987No Hit
TATAGCAGGAGGGGTCCCTCAGCCATCCCGGCTGGCAAAAGCCCCTTGCCAC15690.11835890735643231No Hit
CACAGCAGGAGGGGGCTTTCTGATGAGATCATTGGCTGTGGACGTGGGGACC15660.11813259969418292No Hit
GGTTGCAGGAGGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATG15630.11790629203193351No Hit
TAGGGCAGGAGGGGTATCAACGCAGAGGGGGGAAACAGACTAGGCGAAGTTC15560.11737824082001828No Hit
TTGGGCAGGAGGGGGACAAGATGGCGGCTGGCGGGGCTGTTGCTGTGGCGCC15510.11700106138293595No Hit
GCCAGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG15490.11685018960810303No Hit
TCGGGCAGGAGGGATTGATGCTCGGATGAAGAGGCTGGGAAAGTGAGCATCA15480.11677475372068656No Hit
TTTCGCAGGAGGGGGGTGAACGGATCGAAGATGGCCGTGAGCTTGTTCTGAG15020.11330470289952921No Hit
GTTTGCAGGAGGGACCAGAGGCAGGGATGCTCCCAGCCAGAACAGGTCAAGT14920.11255034402536455No Hit
GCATGCAGGAGGGGGGACGCAGAGTTCTTGCAGGAGGGCAGGCGGAGATCTT14910.11247490813794811No Hit
CTTGGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC14860.11209772870086578No Hit
CCATGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG14780.11149424160153407No Hit
GCTGGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG14730.11111706216445175No Hit
TCGTGCAGGAGGGACGGTTTCCCTTGGCCCGAGATGCTGCACTGCCACAAGT14710.11096619038961882No Hit
CGGGGCAGGAGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAG14710.11096619038961882No Hit
GGCAGCAGGAGGGGGTATCAACGCAGAGTGATGGCAGGAGGGTGGTGGCATG14660.1105890109525365No Hit
TTGAGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC14660.1105890109525365No Hit
GAGTGCAGGAGGGGGTGCGCTCCAAGATGGAGGAACAGACCCAGCAAATACG14620.11028726740287063No Hit
TTTGGCAGGAGGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAA14600.1101363956280377No Hit
GTCTGCAGGAGGGGAACGACTAATGGAACAAGCCAGCTGCTGGGAGAAGGAG14400.10862767787970842No Hit
TTTGGCAGGAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCATT14300.10787331900554378No Hit
CTCTGCAGGAGGGGGTCGAGCGCCGAGCCACTGATGGCGATGGCTGCCACTG14250.10749613956846146No Hit
TACTGCAGGAGGGAGTGGTATCAACGCAGGGTGGAGGTGGAGGGAGGCACAC14210.1071943960187956No Hit
TTTTGCAGGAGGGGGCGTCCTTTGGCTGTGGACACCGTCGTAGAGTCGTCTG14160.10681721658171328No Hit
CCAGGCAGGAGGGGAGTCCAGAGCCTGCAACCTACTTCCTTCTAGCTGCAGC14150.10674178069429681No Hit
GTTCGCAGGAGGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATAC14070.10613829359496511No Hit
TGAAGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC14060.10606285770754864No Hit
TTCTGCAGGAGGCCAGCCAAGTCTACACAGTAAGACCCTAACTCCCAGCTGA14000.10561024238304986No Hit
TGTCGCAGGAGGGGCCGTTTGCCATGGTGCAGCTCCGGCCCCGCTTGTCGCG13950.10523306294596753No Hit
TGGCGCAGGAGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATG13880.10470501173405228No Hit
TTTGGCAGGAGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC13880.10470501173405228No Hit
CTGGGCAGGAGGGGCAGAGTGAAGGCAGGAGGGACCTTGATCATGTTGCCTG13820.1042523964095535No Hit
GTCGGCAGGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCC13810.10417696052213703No Hit
GCTAGCAGGAGGGGGAGATCTGACAATCATGGTAGAAGGCAAAGGAGAAGCA13790.10402608874730411No Hit
GGCAGCAGGAGGGGGTCTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCT13700.10334716576055594No Hit
TATGGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC13690.10327172987313947No Hit
ATAGGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC13630.10281911454864069No Hit
ATAGGCAGGAGGGGGACAAGCATTCTCTGCTCTGCACCAGCCCGCCCTGGGA13630.10281911454864069No Hit
TCAAGCAGGAGGGACAGACCTCAGCCTTACATAAAGAATACAGCAACTTGGG13610.10266824277380775No Hit
TCTAGCAGGAGGGTTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCA13590.10251737099897483No Hit
GCCGGCAGGAGGGGGCCGACTGGGAGCGACCGAGCGGGCCACCGCTGCCGCC13520.10198931978705958No Hit
TTGGGCAGGAGGGATTTACTTTGGTGCTTTGATAGACAGAACCTGTTTACAC13440.10138583268772786No Hit
TTCGGCAGGAGGGAGTTGGGGGTCGGGCCTGGCGGCTCACCTGTAATTCCAG13430.10131039680031141No Hit
TGGTGCAGGAGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCT13420.10123496091289494No Hit
TATGGCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG13420.10123496091289494No Hit
ATCTGCAGGAGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC13340.10063147381356322No Hit
ACACGCAGGAGGGATTCCAGGATTCATGTGCCAGGGTGGTGACTTTACACGC13270.10010342260164797No Hit
AGTTGCAGGAGGGATCGCCAGTGGGCAAAACCTGATGGAGAAGATACAGGCC13260.10002798671423152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAC1050.046.00000425
AAGTACC1050.046.00000426
CAGCGAA1050.046.00000445
CCTTACT1050.046.00000417
TACCCTA1050.046.00000429
TCCATAC1050.046.00000431
ACTGCGT1050.046.00000437
CGAAAGT1050.046.00000423
ACTGAGG2950.046.00000442
TAGGTCG1050.046.00000434
TGGAATA1050.046.00000427
AACTTCA2100.046.00000428
GCTATTA1050.046.00000427
GGACTAA2950.046.00000412
ACTTCAG2100.046.00000429
CTCCATA1050.046.00000430
CATCTAC1050.046.00000425
TTACTAG1050.046.00000419
GCCGCGA1050.046.00000417
CCGTCAA1050.046.00000431
Produced by FastQC (version 0.11.5)