FastQCFastQC Report
Fri 17 Jun 2016
SRR1528073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315634
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGCAGGAGGGATAAACCCTTGGTTTGTACCTCGAATCTTGCGCCTGTTT50720.38551755275403343No Hit
CATTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG47450.3606626158946941No Hit
GCAAGCAGGAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGC47390.3602065620073668No Hit
CCCGGCAGGAGGGGTTGCAACCCGCGGAGGACGAGATTCTCAGGTGCTGGGG33680.25599824875307264No Hit
CTTGGCAGGAGGGGGGAGGCAGAGGCGAGTGCAGCACGAGGTCAAGAGATTG33020.2509816559924721No Hit
CCTTGCAGGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG26480.2012717822737935No Hit
CAGAGCAGGAGGGGGCTAGCTGGTGCGCTCTGTGACTTGATTGCTGCTGCTT25380.19291079433945915No Hit
ATGAGCAGGAGGGCAAGCTGTGCCGCTGCCCACCGAAAGGGCAGCAGCAGCA24850.18888231833473443No Hit
AATTGCAGGAGGGGGTGGCCCACAGGAGAGCACAGGCCCAGACTGAGGGACT24580.18683007584176145No Hit
CTTTGCAGGAGGGAGCCTGGGATAAATGAGACCCCGTCTCAAAATTTAAAGA24570.18675406686054025No Hit
CTTTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG24480.18606998602954924No Hit
CCTTGCAGGAGGGAAGCAGTGGTATCAACGGGGGCCGGTAGTGGTGGCGCAC24100.18318164474314286No Hit
CCTGGCAGGAGGGGCCTGCGGACTAGCGGTGGCTTCGCGTGGGTGGAGGAGG24070.1829536177994792No Hit
GTCGGCAGGAGGGGCCAGGCTGGTCGCGAACTCCTGACCTCAAGTGATCCAC23930.18188949206238209No Hit
GTTAGCAGGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCC22600.1717802975599597No Hit
GCTTGCAGGAGGGATTAGTCCTTTCACAATTAAAAATGTGTTATGAGACTTC22530.17124823469141112No Hit
TTTTGCAGGAGGGGTGGCCCTGGTGCTCACACATGTAATCCCAGCACTTTGG22270.16927200117965938No Hit
TCTGGCAGGAGGGGCTTCCACCTTCCCCCACCCTCCTCATCCAACCTCTGTT22240.16904397423599574No Hit
GCTTGCAGGAGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCT21790.16562357008104078No Hit
GCAGGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG21660.1646354533251649No Hit
CTTGGCAGGAGGGGGGAGGACGGACCGCAGAGGTTGTCTGAAGGCCGAGGCC21570.1639513724941739No Hit
GTAAGCAGGAGGGGGTGCCCCCGACCTACAACTTGCGCCAATTATCGAGCTG21140.16068298630166142No Hit
AGTCGCAGGAGGGGGCTTTTAAAGGCGCTAGCTGGTGCGCTCTGTGACTTGA21010.15969486954578554No Hit
GTTTGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC19980.15186594447999974No Hit
GCTAGCAGGAGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAG19820.15064980078046022No Hit
CTTTGCAGGAGGGGTCTAGCAGTGACCGACCGTCGGAGGCGGCGCGGCCGGG19370.14722939662550527No Hit
GAGTGCAGGAGGGCAAGTACTGGGATACAGCCACCCTTTCAACCCAGCACTC19200.1459372439447445No Hit
TCCTGCAGGAGGGGCAGAGCAGAGAGGAGCAGCGGGCTTGTCGTTGGTGGCG18940.14396101043299275No Hit
GGTAGCAGGAGGGAGTGAACGCAGGAGGGGGCACTGCTGCTGCTGCCACTGG18800.14289688469589565No Hit
GTTGGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC18730.1423648218273471No Hit
ATATGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG18490.14054060627803783No Hit
TGAAGCAGGAGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCT18440.14016056137193172No Hit
AGACGCAGGAGGGAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACAC18130.13780428295407385No Hit
TATCGCAGGAGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC17980.13666414823575554No Hit
CCGGGCAGGAGGGGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACT17910.136132085367207No Hit
ATTTGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC17770.1350679596301099No Hit
CGTAGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG17730.134763923705225No Hit
TATTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG17530.13324374408080059No Hit
ACAGGCAGGAGGGGACCAGCTTGGTCAACATGGTGAAACTCTGTCTCTACTA17260.1311915015878276No Hit
TCTTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG17200.1307354477005003No Hit
GCCCGCAGGAGGGGCTTTTCCTCTTCCGGGGACGGTGCTCAGAGGCACTCAG17140.13027939381317297No Hit
CGTGGCAGGAGGGGGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGC17080.12982333992584563No Hit
CATAGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG17040.12951930400096073No Hit
TCTTGCAGGAGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC16930.12868320520752732No Hit
ACCAGCAGGAGGGGGAGAAAGCCAGAGCCGGTTTGCAGGCAACGCTGTGGGG16740.12723903456432412No Hit
GCTGGCAGGAGGGGGCTCCTGGTGCTGCTTGTGCGCTCCCTCAGTGCGGACC16740.12723903456432412No Hit
CACTGCAGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG16730.1271630255831029No Hit
GGCAGCAGGAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGC16560.12587087290234214No Hit
GCGTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG16550.1257948639211209No Hit
TTGTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG16550.1257948639211209No Hit
GCACGCAGGAGGGGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTT16300.1238946393905904No Hit
CCTTGCAGGAGGGGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACT16210.1232105585595994No Hit
TTGGGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC16070.12214643282250232No Hit
GATGGCAGGAGGGGCTCTGTTGAGGTTTTTTCTTCAGCAAGTACACCATGTC16030.12184239689761742No Hit
GGAAGCAGGAGGGAGATCAGGAATTAGAGACCAGCCTGGCCAATACAATATG15960.12131033402906888No Hit
GGAGGCAGGAGGGAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAG15940.12115831606662644No Hit
CTTTGCAGGAGGGGAAGGTGCTCGGTCCTTCCGAGGAAGCTAAGGCCGCGTT15930.12108230708540522No Hit
TAGGGCAGGAGGGGTATCAACGCAGAGGGGGGAAACAGACTAGGCGAAGTTC15930.12108230708540522No Hit
TTGGGCAGGAGGGGGTCTCTCATGAGACTCTGAAGGGTGGAAGGAAGAAGAC15880.12070226217929911No Hit
TGGGGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG15870.12062625319807789No Hit
AATCGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG15860.12055024421685667No Hit
ATGAGCAGGAGGGGCGGGTAGATCACTTGAGGCCAGTTCAAGACCAGCCTGA15730.1195621274609808No Hit
AGTTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG15690.1192580915360959No Hit
CATCGCAGGAGGGGGAGACTGAGTACACCTTCCCACTTACTGAAAAGGTCAA15360.11674979515579562No Hit
GGTTGCAGGAGGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATG15300.11629374126846828No Hit
TTTCGCAGGAGGGGGGTGAACGGATCGAAGATGGCCGTGAGCTTGTTCTGAG15260.11598970534358338No Hit
GTTTGCAGGAGGGACCAGAGGCAGGGATGCTCCCAGCCAGAACAGGTCAAGT15250.11591369636236218No Hit
GGCAGCAGGAGGGGGTATCAACGCAGAGTGATGGCAGGAGGGTGGTGGCATG15100.11477356164404386No Hit
TATAGCAGGAGGGGTCCCTCAGCCATCCCGGCTGGCAAAAGCCCCTTGCCAC15070.11454553470038019No Hit
CACAGCAGGAGGGGGCTTTCTGATGAGATCATTGGCTGTGGACGTGGGGACC15030.11424149877549532No Hit
CATGGCAGGAGGGATGTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGA15000.11401347183183164No Hit
GTTCGCAGGAGGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATAC15000.11401347183183164No Hit
TTCTGCAGGAGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC14990.11393746285061042No Hit
GCCAGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG14880.11310136405717701No Hit
TCGTGCAGGAGGGACGGTTTCCCTTGGCCCGAGATGCTGCACTGCCACAAGT14820.11264531016984967No Hit
TTGAGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC14810.11256930118862846No Hit
CCAGGCAGGAGGGGAGTCCAGAGCCTGCAACCTACTTCCTTCTAGCTGCAGC14730.11196122933885867No Hit
TTGGGCAGGAGGGGGACAAGATGGCGGCTGGCGGGGCTGTTGCTGTGGCGCC14720.11188522035763745No Hit
CCATGCAGGAGGGGCAGAGTCGCGCAGGAGGGGCTTTTTTTCCGACATCTTA14690.1116571934139738No Hit
GCATGCAGGAGGGGGGACGCAGAGTTCTTGCAGGAGGGCAGGCGGAGATCTT14650.1113531574890889No Hit
GGCAGCAGGAGGGGGTCTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCT14610.11104912156420402No Hit
TCGGGCAGGAGGGATTGATGCTCGGATGAAGAGGCTGGGAAAGTGAGCATCA14480.11006100480832816No Hit
TTCTGCAGGAGGCCAGCCAAGTCTACACAGTAAGACCCTAACTCCCAGCTGA14470.10998499582710693No Hit
TACTGCAGGAGGGAGTGGTATCAACGCAGGGTGGAGGTGGAGGGAGGCACAC14340.10899687907123105No Hit
CCATGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG14330.10892087009000984No Hit
GAGTGCAGGAGGGGGTGCGCTCCAAGATGGAGGAACAGACCCAGCAAATACG14270.1084648162026825No Hit
TTTGGCAGGAGGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAA14240.10823678925901885No Hit
TGAAGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC14230.10816078027779763No Hit
TTTGGCAGGAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCATT14140.10747669944680663No Hit
CTGGGCAGGAGGGGCAGAGTGAAGGCAGGAGGGACCTTGATCATGTTGCCTG14100.10717266352192176No Hit
CTTGGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC14020.10656459167215199No Hit
TGTCGCAGGAGGGGCCGTTTGCCATGGTGCAGCTCCGGCCCCGCTTGTCGCG13940.1059565198223822No Hit
TTTTGCAGGAGGGGGCGTCCTTTGGCTGTGGACACCGTCGTAGAGTCGTCTG13930.10588051084116099No Hit
GTCTGCAGGAGGGGAACGACTAATGGAACAAGCCAGCTGCTGGGAGAAGGAG13930.10588051084116099No Hit
ATAGGCAGGAGGGGGACAAGCATTCTCTGCTCTGCACCAGCCCGCCCTGGGA13900.10565248389749733No Hit
GCTGGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG13900.10565248389749733No Hit
CTCTGCAGGAGGGGGTCGAGCGCCGAGCCACTGATGGCGATGGCTGCCACTG13760.10458835816040024No Hit
TATGGCAGGAGCCTTCATTCTTGTGCTACTGTAATTTCTCACTTCCTTATCA13710.10420831325429414No Hit
TGGCGCAGGAGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATG13610.1034482234420819No Hit
TATGGCAGGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG13600.10337221446086069No Hit
GGTAGCAGGAGGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATG13580.10322019649841825No Hit
CGGGGCAGGAGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAG13540.10291616057353337No Hit
GTCGGCAGGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCC13410.1019280438176575No Hit
TCTAGCAGGAGGGTTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCA13400.10185203483643628No Hit
TCAAGCAGGAGGGACAGACCTCAGCCTTACATAAAGAATACAGCAACTTGGG13380.10170001687399384No Hit
CTTGGCAGGAGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCT13370.10162400789277262No Hit
AGTTGCAGGAGGGATCGCCAGTGGGCAAAACCTGATGGAGAAGATACAGGCC13370.10162400789277262No Hit
TTCGGCAGGAGGGAGTTGGGGGTCGGGCCTGGCGGCTCACCTGTAATTCCAG13260.10078790909933918No Hit
TATGGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC13240.10063589113689675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAT405.6115823E-946.00000413
TCACGGT700.046.00000443
TCCGGAG206.311788E-446.00000442
AGCACAC405.6115823E-946.00000436
ATTTATG351.01972546E-746.00000422
TTTAGTG206.311788E-446.00000415
ATTTAGT206.311788E-446.00000414
GTGTTCC700.046.00000424
GTCATGC405.6115823E-946.00000446
CTAGATT551.8189894E-1246.00000442
GTGTTAC206.311788E-446.00000438
ATCCTCT551.8189894E-1246.00000431
GTCATCA1500.046.00000417
ATCCTAT700.046.00000442
CTATGTT551.8189894E-1246.00000434
AAGTATA1600.046.00000434
TTAGGTC405.6115823E-946.00000442
TTTAGAG206.311788E-446.00000426
TATCAGA206.311788E-446.00000422
AACCGTT351.01972546E-746.00000431
Produced by FastQC (version 0.11.5)