FastQCFastQC Report
Fri 17 Jun 2016
SRR1528072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118644
Sequences flagged as poor quality0
Sequence length59
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAGCAGGAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG43760.3911879024962366No Hit
TTAAGCAGGAGGGATAAACCCTTGGTTTGTACCTCGAATCTTGCGCCTGTTTCTTCCCC40040.35793335502626394No Hit
CATTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG37520.3354060809337019No Hit
CTTTGCAGGAGGGAGCCTGGGATAAATGAGACCCCGTCTCAAAATTTAAAGAGAAAGAA23750.2123106189279163No Hit
CCTTGCAGGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG23490.2099863763628107No Hit
CCTTGCAGGAGGGAAGCAGTGGTATCAACGGGGGCCGGTAGTGGTGGCGCACGCCGGTA23460.20971819452837542No Hit
GCTTGCAGGAGGGATTAGTCCTTTCACAATTAAAAATGTGTTATGAGACTTCAAAGAAT21140.18897879933204845No Hit
AATTGCAGGAGGGGGTGGCCCACAGGAGAGCACAGGCCCAGACTGAGGGACTCTTCTAT20850.18638637493250756No Hit
CAGAGCAGGAGGGGGCTAGCTGGTGCGCTCTGTGACTTGATTGCTGCTGCTTCTGAGCT20600.1841515263122137No Hit
ATGAGCAGGAGGGCAAGCTGTGCCGCTGCCCACCGAAAGGGCAGCAGCAGCAACGAGCC19760.17664243494802637No Hit
CTTGGCAGGAGGGGGGAGGCAGAGGCGAGTGCAGCACGAGGTCAAGAGATTGAGGTCAT19710.1761954652239676No Hit
CTTTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG19230.17190455587300338No Hit
GCTTGCAGGAGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCA17200.15375758507621728No Hit
TCTGGCAGGAGGGGCTTCCACCTTCCCCCACCCTCCTCATCCAACCTCTGTTTGGCCCT17130.153131827462535No Hit
GTAAGCAGGAGGGGGTGCCCCCGACCTACAACTTGCGCCAATTATCGAGCTGGTAGACG17010.15205910012479396No Hit
GTTAGCAGGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCCAAGTGCT16700.14928788783562957No Hit
TTTTGCAGGAGGGGTGGCCCTGGTGCTCACACATGTAATCCCAGCACTTTGGGAGGCTG16640.14875152416675905No Hit
CTTTGCAGGAGGGGTCTAGCAGTGACCGACCGTCGGAGGCGGCGCGGCCGGGAGGCAAC16590.14830455444270027No Hit
AGTCGCAGGAGGGGGCTTTTAAAGGCGCTAGCTGGTGCGCTCTGTGACTTGATTGCTGC16540.1478575847186415No Hit
ATATGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG16520.147678796829018No Hit
GAGTGCAGGAGGGCAAGTACTGGGATACAGCCACCCTTTCAACCCAGCACTCTGAAGAC16490.14741061499458272No Hit
GCTAGCAGGAGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAGATGGGCG15800.14124243280257168No Hit
GTCGGCAGGAGGGGCCAGGCTGGTCGCGAACTCCTGACCTCAAGTGATCCACCCACCTC15480.13838182656859555No Hit
GCAGGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG15430.1379348568445368No Hit
GGAAGCAGGAGGGAGATCAGGAATTAGAGACCAGCCTGGCCAATACAATATGGCAAAAC15190.1357894021690547No Hit
CTTGGCAGGAGGGGGGAGGACGGACCGCAGAGGTTGTCTGAAGGCCGAGGCCAAGATGG15140.1353424324449959No Hit
GGTAGCAGGAGGGAGTGAACGCAGGAGGGGGCACTGCTGCTGCTGCCACTGGATTGAAA14900.1331969777695138No Hit
CATCGCAGGAGGGGGAGACTGAGTACACCTTCCCACTTACTGAAAAGGTCAAGGCCTTC14840.13266061410064328No Hit
CCGGGCAGGAGGGGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTCTTC14830.13257122015583153No Hit
CATAGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG14730.13167728070771398No Hit
TGAAGCAGGAGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA14380.12854849263930257No Hit
GTTGGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAAC14240.12729697741193802No Hit
GTTTGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAAC14240.12729697741193802No Hit
GTTCGCAGGAGGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCGCCTA14090.12595606823976171No Hit
CACTGCAGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAG14040.12550909851570294No Hit
GCGTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG14010.1252409166812677No Hit
TATCGCAGGAGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGG13960.12479394695720891No Hit
TATTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG13930.12452576512277365No Hit
CCCGGCAGGAGGGGTTGCAACCCGCGGAGGACGAGATTCTCAGGTGCTGGGGGCAGGGT13850.12381061356427961No Hit
ACCAGCAGGAGGGGGAGAAAGCCAGAGCCGGTTTGCAGGCAACGCTGTGGGGATCGGGC13810.12345303778503258No Hit
AGACGCAGGAGGGAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTG13720.1226484922817268No Hit
CCTGGCAGGAGGGGCCTGCGGACTAGCGGTGGCTTCGCGTGGGTGGAGGAGGCGCGGCT13450.12023485577180944No Hit
TGGGGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG13400.11978788604775066No Hit
GCACGCAGGAGGGGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGG13310.11898334054444488No Hit
CACAGCAGGAGGGGGCTTTCTGATGAGATCATTGGCTGTGGACGTGGGGACCTGAGTGG13310.11898334054444488No Hit
GCCCGCAGGAGGGGCTTTTCCTCTTCCGGGGACGGTGCTCAGAGGCACTCAGGAGGGTC13180.11782121926189207No Hit
TCCTGCAGGAGGGGCAGAGCAGAGAGGAGCAGCGGGCTTGTCGTTGGTGGCGGTGGCGG13110.1171954616482098No Hit
TCTTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG13020.116390916144904No Hit
CCTTGCAGGAGGGGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTCTTC12990.11612273431046874No Hit
CGTAGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG12760.1140666735797984No Hit
GGCAGCAGGAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCT12670.11326212807649262No Hit
AATCGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG12650.11308334018686911No Hit
CCATGCAGGAGGGGAAATGGTAAAGAGGATCTGCAAAAACAAAACCAGGTTCTTTGAAA12630.1129045522972456No Hit
TTCTGCAGGAGGCCAGCCAAGTCTACACAGTAAGACCCTAACTCCCAGCTGAGCGTGGT12630.1129045522972456No Hit
CCATGCAGGAGGGGCAGAGTCGCGCAGGAGGGGCTTTTTTTCCGACATCTTAGCGAGGC12590.11254697651799858No Hit
TCAAGCAGGAGGGACAGACCTCAGCCTTACATAAAGAATACAGCAACTTGGGCTGGAGA12510.11183182495950454No Hit
TACCGCAGGAGGGCTCTGCATTTCACTCCGATAATTTTTCCAGACTCTCCTGAATCCTG12500.11174243101469278No Hit
ATTTGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAAC12330.11022273395289296No Hit
GGCAGCAGGAGGGGGTATCAACGCAGAGTGATGGCAGGAGGGTGGTGGCATGCACCTGT12280.10977576422883419No Hit
GCCAGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG12170.1087924308359049No Hit
GGAGGCAGGAGGGAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCG12130.10843485505665788No Hit
TTGGGCAGGAGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAAC12090.10807727927741087No Hit
GCATGCAGGAGGGGGGACGCAGAGTTCTTGCAGGAGGGCAGGCGGAGATCTTCCAGGCC12080.1079878853325991No Hit
CACTGCAGGAGGGAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA12050.10771970349816384No Hit
GCTGGCAGGAGGGGGCTCCTGGTGCTGCTTGTGCGCTCCCTCAGTGCGGACCCGGTGCC12030.10754091560854033No Hit
TTGTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG12010.10736212771891684No Hit
CCATGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG12000.10727273377410507No Hit
ATCTGCAGGAGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCG11950.10682576405004629No Hit
TTGGGCAGGAGGGGGTCTCTCATGAGACTCTGAAGGGTGGAAGGAAGAAGACTGGAAAA11930.1066469761604228No Hit
GAGTGCAGGAGGGGGTGCGCTCCAAGATGGAGGAACAGACCCAGCAAATACGCCTGCAG11920.10655758221561104No Hit
CTGGGCAGGAGGGGCAGAGTGAAGGCAGGAGGGACCTTGATCATGTTGCCTGCATTCCT11920.10655758221561104No Hit
TTCTGCAGGAGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGG11810.10557424882268174No Hit
AGTTGCAGGAGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTG11710.10468030937456421No Hit
TATAGCAGGAGGGGTCCCTCAGCCATCCCGGCTGGCAAAAGCCCCTTGCCACAACCTTC11690.1045015214849407No Hit
TCGTGCAGGAGGGACGGTTTCCCTTGGCCCGAGATGCTGCACTGCCACAAGTTCCCCCT11630.10396515781607017No Hit
GATGGCAGGAGGGGCTCTGTTGAGGTTTTTTCTTCAGCAAGTACACCATGTCTGGTCGT11600.10369697598163491No Hit
CCAGGCAGGAGGGGAGTCCAGAGCCTGCAACCTACTTCCTTCTAGCTGCAGCCACTTGT11600.10369697598163491No Hit
TACTGCAGGAGGGAGTGGTATCAACGCAGGGTGGAGGTGGAGGGAGGCACACACTGCCT11560.10333940020238788No Hit
TAGGGCAGGAGGGGTATCAACGCAGAGGGGGGAAACAGACTAGGCGAAGTTCTGCTTTG11530.10307121836795263No Hit
AGTTGCAGGAGGGATCGCCAGTGGGCAAAACCTGATGGAGAAGATACAGGCCTCTGTGG11470.1025348546990821No Hit
CTTTGCAGGAGGGGAAGGTGCTCGGTCCTTCCGAGGAAGCTAAGGCCGCGTTGGGGTGA11350.10146212736134107No Hit
TAGTGCAGGAGGGAGTTTCCCTGATGTCCCAGAGTCAAGAGCAGGGATCTGATCCGATG11350.10146212736134107No Hit
TTTGGCAGGAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCATTCTTCTAC11230.1003894000236No Hit
CTCTGCAGGAGGGGGTCGAGCGCCGAGCCACTGATGGCGATGGCTGCCACTGGTCTCTG11210.10021061213397649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAT150.006696218653.00000413
TCACGGT600.053.00000443
CTAGAGA150.006696218653.00000434
AACCGTA600.053.00000422
CCTTATC150.006696218653.00000445
TAGAGCC150.006696218653.00000450
TGATACG307.0103124E-753.00000435
AGTCCGT150.006696218653.00000434
TCGTTAA150.006696218653.00000424
GTATTGT307.0103124E-753.00000438
GATGTAA600.053.00000450
CTGTACC307.0103124E-753.00000437
TCATTTG750.053.00000449
TTTACAT150.006696218653.00000437
GCGGTAA750.053.00000419
CCCTAAG750.053.00000434
ACCGCGT150.006696218653.00000420
CATTGTA750.053.00000453
GTTTAAC307.0103124E-753.00000449
CTATATA150.006696218653.00000416
Produced by FastQC (version 0.11.5)