FastQCFastQC Report
Fri 17 Jun 2016
SRR1528071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5798047
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA1050.046.00000442
TAATACG1200.046.040
TCGTTAC750.046.046
ATAGGTC253.418878E-546.046
ACGTTAA453.110472E-1046.025
TAACGGC2500.046.041
CGCGAAT301.8627161E-646.017
ATCCGTA1250.046.015
ATACTCG301.8627161E-646.025
CGTTACA501.6370905E-1146.031
ATATCTC650.046.017
ACGATAA600.046.042
CCGTAAA405.6188583E-946.043
ATATACG750.046.030
ATATACC1100.046.031
CGTAGAC206.313578E-446.016
TACGAAC253.418878E-546.017
TATTCGC253.418878E-546.015
ACTACGG453.110472E-1046.029
CGAATAC600.046.035
Produced by FastQC (version 0.11.5)