FastQCFastQC Report
Fri 17 Jun 2016
SRR1528070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5764015
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAAT750.046.00000425
CGTATCT750.046.00000434
CGATTCA750.046.00000417
CGTACTA750.046.00000421
ACACGCG501.6370905E-1146.041
CGACGTA301.8627143E-646.032
CGAAAAT301.8627143E-646.027
ACGTTAG850.046.041
ATAGCTA800.046.014
CCAATTA206.313575E-446.033
CGTCTAC1100.046.020
CAATTCG551.8189894E-1246.017
TCGATCA253.4188753E-546.036
ATACTCG253.4188753E-546.025
GTAATAC850.046.039
CGTTAAT2600.046.018
CGTTAAC600.046.034
ACGATTT551.8189894E-1246.034
ACGATTC1000.046.043
ACGATAC551.8189894E-1246.021
Produced by FastQC (version 0.11.5)