##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528070_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5764015
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.054798434771595	31.0	31.0	33.0	30.0	33.0
2	31.70000043372545	31.0	31.0	34.0	30.0	34.0
3	31.950400371962946	31.0	31.0	34.0	30.0	34.0
4	35.72936069736113	37.0	35.0	37.0	35.0	37.0
5	35.366404841069986	37.0	35.0	37.0	33.0	37.0
6	35.566550919801564	37.0	35.0	37.0	33.0	37.0
7	35.84212515061116	37.0	35.0	37.0	35.0	37.0
8	35.92177553320038	37.0	35.0	37.0	35.0	37.0
9	37.120767208274096	39.0	37.0	39.0	33.0	39.0
10	36.13539347139103	37.0	35.0	39.0	32.0	39.0
11	37.19093513809385	39.0	37.0	39.0	34.0	39.0
12	37.44171987755063	39.0	37.0	39.0	35.0	39.0
13	37.38583053652706	39.0	37.0	39.0	35.0	39.0
14	37.99528557784808	40.0	38.0	40.0	33.0	40.0
15	37.324204916191235	39.0	37.0	40.0	32.0	40.0
16	37.031016574384346	39.0	36.0	40.0	32.0	40.0
17	36.98331909268106	39.0	36.0	40.0	32.0	40.0
18	37.129173154476526	39.0	36.0	40.0	32.0	40.0
19	37.27489414930391	39.0	36.0	40.0	32.0	40.0
20	37.401457144022004	39.0	37.0	40.0	32.0	40.0
21	37.35033687455706	39.0	36.0	40.0	32.0	40.0
22	37.38239178766884	39.0	37.0	40.0	32.0	40.0
23	37.45572556629364	40.0	37.0	40.0	33.0	40.0
24	37.42393609315729	40.0	37.0	40.0	32.0	40.0
25	37.42708893019883	40.0	37.0	40.0	32.0	40.0
26	37.157753753243185	40.0	36.0	40.0	32.0	40.0
27	36.973572934837954	40.0	36.0	40.0	31.0	40.0
28	36.94539778262201	40.0	36.0	40.0	31.0	40.0
29	37.01765262581725	40.0	36.0	40.0	31.0	40.0
30	36.885271290931755	39.0	35.0	40.0	31.0	40.0
31	36.888975479765406	39.0	35.0	40.0	31.0	40.0
32	36.943879049586094	39.0	35.0	40.0	31.0	40.0
33	36.95604574242086	39.0	35.0	40.0	31.0	40.0
34	36.88384173184837	39.0	35.0	40.0	31.0	40.0
35	36.65903333006594	39.0	35.0	40.0	31.0	40.0
36	36.664225023703096	39.0	35.0	40.0	31.0	40.0
37	36.54019845541693	39.0	35.0	40.0	31.0	40.0
38	36.36856305890946	38.0	35.0	40.0	31.0	40.0
39	36.22972147019048	38.0	35.0	40.0	31.0	40.0
40	36.0483851967769	38.0	35.0	40.0	30.0	40.0
41	35.88271317822733	38.0	35.0	40.0	30.0	40.0
42	35.68869321124251	38.0	35.0	40.0	30.0	40.0
43	35.51837425822105	37.0	34.0	40.0	29.0	40.0
44	35.2201288511567	37.0	34.0	40.0	28.0	40.0
45	35.08716528322706	37.0	34.0	40.0	28.0	40.0
46	34.9553845713448	37.0	34.0	40.0	28.0	40.0
47	34.80918248824821	36.0	34.0	39.0	28.0	40.0
48	34.66723889511044	36.0	34.0	39.0	28.0	40.0
49	34.351036907433446	35.0	33.0	39.0	27.0	40.0
50	34.23017098324692	35.0	33.0	39.0	27.0	40.0
51	34.06896980663652	35.0	33.0	39.0	26.0	40.0
52	32.87077375752839	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	4.0
14	21.0
15	29.0
16	80.0
17	234.0
18	746.0
19	1632.0
20	3088.0
21	5514.0
22	9285.0
23	14548.0
24	21208.0
25	30664.0
26	43286.0
27	58471.0
28	78978.0
29	104113.0
30	132972.0
31	166114.0
32	203784.0
33	260415.0
34	364644.0
35	519582.0
36	665572.0
37	1110620.0
38	1662239.0
39	306169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.33910737567477	19.649515138319384	31.41971351566573	16.591663970340118
2	30.42214498053874	19.951578890755837	33.52336869352352	16.1029074351819
3	22.31987598921932	21.298227017105265	37.22259570802643	19.15930128564898
4	20.880705549864114	22.059710115258202	38.623112535272725	18.436471799604963
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.29347859087805	0.6612751701721803	0.03591246726457166	0.00933377168518819
12	99.43431791901999	0.4433715040644412	0.08957297994540264	0.032737596970167496
13	97.62438161593958	1.5728272740442208	0.3225008956430544	0.4802902143731409
14	63.28685820560842	24.082345379045684	5.2025020753762785	7.428294339969622
15	31.95185994484747	27.918872522018074	17.599988896628478	22.52927863650598
16	27.62881429003915	17.94957160937298	29.80826732754859	24.61334677303928
17	22.052180641445243	17.713572917488936	30.517720720712905	29.716525720352916
18	22.387658602553948	18.62993069934759	31.624067598713744	27.35834309938472
19	23.867217555818296	18.28569148414777	30.28326609143106	27.563824868602875
20	27.495816718034217	18.467301004594887	25.873787628935734	28.163094648435163
21	29.19910860745505	18.77479499966603	21.55530476586199	30.47079162701693
22	29.138560534627338	20.708950271642248	23.224228250620442	26.928260943109965
23	30.142444112307132	19.51814837400666	20.586743788834696	29.752663724851512
24	28.280703641472133	21.015698258939295	21.87777096346904	28.825827136119525
25	31.115463786961	18.78515236341335	22.665381682733305	27.434002166892345
26	30.38186056073761	20.192157723392462	20.98396690501326	28.44201481085667
27	30.18992837457918	21.399285046968128	20.60525519104305	27.805531387409644
28	29.852212390148186	21.08146838618567	20.085530658750887	28.980788564915255
29	28.872860323923515	21.3684211439422	20.615733997916383	29.142984534217902
30	29.693052498995925	20.21476349384934	21.21217588781431	28.880008119340424
31	30.105698892178456	20.801854263044074	20.998626825225124	28.093820019552346
32	30.708802804989233	22.164289995775512	19.39608068334312	27.730826515892133
33	30.73067991669002	22.731325300159696	19.63488991614352	26.903104867006768
34	29.224611663918292	21.98169158130227	20.42883302697859	28.36486372780085
35	29.74503015693054	23.874799076685264	19.99574949058946	26.38442127579474
36	32.21265038345667	22.43660018233818	19.262632036870134	26.08811739733502
37	31.54155566909524	22.70315049492411	20.31696655890035	25.438327277080298
38	30.17965775592187	24.001984727659455	22.015817099712613	23.802540416706066
39	31.425022315174406	22.527682526849773	22.45915390574105	23.58814125223477
40	30.611266625780814	24.375509085247003	20.366480656278654	24.64674363269353
41	32.28221994564552	23.235695951519904	19.558935915329855	24.92314818750472
42	31.52639262736131	24.347074044741383	19.613880255342846	24.51265307255446
43	31.688172220231902	23.001640349652106	19.792332254513564	25.51785517560242
44	30.893986223144804	23.59551458488571	21.346474636169408	24.164024555800083
45	28.842829173761693	23.857276568503032	20.881555651746222	26.418338605989057
46	28.804661334156833	22.819493009646923	21.056815431604534	27.31903022459171
47	29.122512692975295	21.326419171358854	23.577887982595467	25.973180153070384
48	28.85459180796719	21.28939636694214	22.02246177360746	27.833550051483215
49	30.12080988685838	20.560199791291314	22.25948405755363	27.059506264296672
50	27.847325171776966	21.877701567397033	22.33529926622328	27.93967399460272
51	28.68210787098923	22.658181840262387	21.576782850148724	27.082927438599658
52	29.587466375434484	22.07587940003626	22.07133395731968	26.265320267209574
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	6.5
4	10.0
5	116.0
6	222.0
7	855.5
8	1489.0
9	2162.5
10	2836.0
11	3896.0
12	4956.0
13	5812.0
14	6521.0
15	6374.0
16	7233.5
17	8093.0
18	9405.0
19	10717.0
20	13949.0
21	17181.0
22	22378.0
23	27575.0
24	33191.0
25	38807.0
26	42245.0
27	45683.0
28	46203.0
29	46723.0
30	46183.0
31	45643.0
32	52056.5
33	58470.0
34	60644.0
35	62818.0
36	67068.0
37	71318.0
38	81680.5
39	99001.0
40	105959.0
41	121426.0
42	136893.0
43	166687.0
44	196481.0
45	211049.5
46	225618.0
47	249443.5
48	273269.0
49	292959.5
50	312650.0
51	375480.5
52	438311.0
53	484412.5
54	530514.0
55	539346.5
56	548179.0
57	560549.5
58	572920.0
59	562603.0
60	552286.0
61	482874.0
62	413462.0
63	361841.5
64	259868.0
65	209515.0
66	186290.0
67	163065.0
68	132685.5
69	102306.0
70	89604.5
71	76903.0
72	54539.0
73	32175.0
74	20481.5
75	8788.0
76	6213.0
77	3638.0
78	3369.5
79	3101.0
80	1603.0
81	105.0
82	57.5
83	10.0
84	7.5
85	5.0
86	8.5
87	12.0
88	6.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5764015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.69012192310263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06860993246192	6.080472506708758
2	7.9988529086405675	1.2302430822482013
3	2.951959951286907	0.6810279581253725
4	1.7610111846392142	0.5416956287129182
5	1.2011679178199444	0.4618563869077347
6	0.8638854949321984	0.39860308701770797
7	0.6716249869267396	0.3615414625248186
8	0.4762573790599839	0.2929981849398866
9	0.38932802781136133	0.26945820017554145
>10	2.319433922523748	3.475687530314602
>50	0.29615329668528817	1.6205426449988907
>100	1.068319376894016	24.57408325660861
>500	0.7350461680143026	39.162867796842264
>1k	0.19811881340580043	20.750130456470085
>5k	2.3063889802770712E-4	0.09879181740460083
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0022206743042826917	0.0	0.0	0.0	0.0
12	0.008587763911093222	0.0	0.0	0.0	0.0
13	0.01795623363228583	0.0	0.0	0.0	0.0
14	0.06561398608435266	0.0	0.0	0.0	0.0
15	0.10556877454343891	0.0	0.0	0.0	0.0
16	0.12290044352764523	0.0	0.0	0.0	0.0
17	0.13802878722557108	0.0	0.0	0.0	0.0
18	0.15445830727366255	0.0	0.0	0.0	0.0
19	0.17359427413009856	0.0	0.0	0.0	0.0
20	0.19399671930069579	0.0	0.0	0.0	0.0
21	0.21566564278545425	0.0	0.0	0.0	0.0
22	0.24208819720281782	0.0	0.0	0.0	0.0
23	0.2627855756794526	0.0	0.0	0.0	0.0
24	0.28468003639823974	0.0	0.0	0.0	0.0
25	0.3104433281315194	0.0	0.0	0.0	0.0
26	0.34017954498730485	0.0	0.0	0.0	0.0
27	0.36708787190873027	0.0	0.0	0.0	0.0
28	0.3960260339364141	0.0	0.0	0.0	0.0
29	0.433708101037211	0.0	0.0	0.0	0.0
30	0.48067189276918953	0.0	0.0	0.0	0.0
31	0.5186315441580218	0.0	0.0	0.0	0.0
32	0.5435967810631999	0.0	0.0	0.0	0.0
33	0.5666709750061372	0.0	0.0	0.0	0.0
34	0.5887389259049465	0.0	0.0	0.0	0.0
35	0.6098006337596277	0.0	0.0	0.0	0.0
36	0.6334126472606334	0.0	0.0	0.0	0.0
37	0.6540059316292549	0.0	0.0	0.0	0.0
38	0.6758830433300399	0.0	0.0	0.0	0.0
39	0.7004145547851628	0.0	0.0	0.0	0.0
40	0.72647277982448	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTAAT	75	0.0	46.000004	25
CGTATCT	75	0.0	46.000004	34
CGATTCA	75	0.0	46.000004	17
CGTACTA	75	0.0	46.000004	21
ACACGCG	50	1.6370905E-11	46.0	41
CGACGTA	30	1.8627143E-6	46.0	32
CGAAAAT	30	1.8627143E-6	46.0	27
ACGTTAG	85	0.0	46.0	41
ATAGCTA	80	0.0	46.0	14
CCAATTA	20	6.313575E-4	46.0	33
CGTCTAC	110	0.0	46.0	20
CAATTCG	55	1.8189894E-12	46.0	17
TCGATCA	25	3.4188753E-5	46.0	36
ATACTCG	25	3.4188753E-5	46.0	25
GTAATAC	85	0.0	46.0	39
CGTTAAT	260	0.0	46.0	18
CGTTAAC	60	0.0	46.0	34
ACGATTT	55	1.8189894E-12	46.0	34
ACGATTC	100	0.0	46.0	43
ACGATAC	55	1.8189894E-12	46.0	21
>>END_MODULE