FastQCFastQC Report
Fri 17 Jun 2016
SRR1528069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5044197
Sequences flagged as poor quality0
Sequence length59
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA650.053.032
ACGTTTA1650.053.029
CCGATTA503.6379788E-1253.019
ACGTTAA203.1392663E-453.025
CCGATAC503.6379788E-1253.042
CGCATAA307.01526E-753.033
CATTACG550.053.016
TTATACG550.053.047
CGGATTA503.6379788E-1253.042
ACTACGT251.4794534E-553.039
TTAGTAC203.1392663E-453.050
TTACGCG401.6025297E-953.025
TACTACG203.1392663E-453.015
ACCGTTC150.006697575653.024
TTACACG503.6379788E-1253.045
CACGATA850.052.99999620
CGTATAT17550.052.547011
CGATATT40600.052.5431022
CGTTTAT30550.052.3060531
CCGTTAT14500.051.1724131
Produced by FastQC (version 0.11.5)