##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528069_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5044197
Sequences flagged as poor quality	0
Sequence length	59
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.630841341049923	31.0	31.0	31.0	28.0	33.0
2	31.2495041331653	31.0	31.0	33.0	30.0	34.0
3	31.595393280635154	31.0	31.0	34.0	30.0	34.0
4	35.464203519410525	37.0	35.0	37.0	33.0	37.0
5	34.948780945708506	35.0	35.0	37.0	32.0	37.0
6	35.174393664640775	36.0	35.0	37.0	33.0	37.0
7	35.46102481723057	37.0	35.0	37.0	33.0	37.0
8	35.602635662326435	37.0	35.0	37.0	33.0	37.0
9	36.51155674530555	38.0	35.0	39.0	32.0	39.0
10	35.64012428539171	37.0	35.0	39.0	32.0	39.0
11	36.5119807969435	37.0	35.0	39.0	33.0	39.0
12	36.86272859684108	39.0	37.0	39.0	33.0	39.0
13	36.890961237239544	39.0	37.0	39.0	32.0	39.0
14	37.24037146051195	39.0	37.0	40.0	32.0	40.0
15	36.399802981525106	38.0	36.0	40.0	30.0	40.0
16	35.90983639219483	38.0	35.0	40.0	30.0	40.0
17	36.05058386894881	38.0	35.0	40.0	30.0	40.0
18	36.40953535319893	38.0	35.0	40.0	30.0	40.0
19	36.62509870252887	39.0	36.0	40.0	31.0	40.0
20	36.66762380612811	39.0	35.0	40.0	31.0	40.0
21	36.542529762418084	39.0	35.0	40.0	30.0	40.0
22	36.53801288887012	39.0	35.0	40.0	30.0	40.0
23	36.521082146474455	39.0	35.0	40.0	30.0	40.0
24	36.458823277520686	39.0	35.0	40.0	30.0	40.0
25	36.46947135490545	39.0	35.0	40.0	30.0	40.0
26	36.12198512468883	38.0	35.0	40.0	30.0	40.0
27	35.92235077258085	38.0	35.0	40.0	29.0	40.0
28	35.843109815100405	38.0	35.0	40.0	29.0	40.0
29	35.81549412126449	38.0	35.0	40.0	29.0	40.0
30	35.73107691868498	38.0	35.0	40.0	28.0	40.0
31	35.690290050130876	38.0	34.0	40.0	28.0	40.0
32	35.65532551563708	38.0	34.0	40.0	28.0	40.0
33	35.41301717597469	38.0	34.0	40.0	27.0	40.0
34	35.28884260468019	38.0	34.0	40.0	27.0	40.0
35	35.190704486759735	38.0	34.0	40.0	26.0	40.0
36	35.02403772096926	38.0	33.0	40.0	26.0	40.0
37	34.98993258986515	38.0	33.0	40.0	26.0	40.0
38	34.86366690277957	37.0	33.0	40.0	26.0	40.0
39	34.631890070907225	37.0	33.0	40.0	26.0	40.0
40	34.509629580288	37.0	33.0	40.0	25.0	40.0
41	34.282417399637644	37.0	33.0	40.0	24.0	40.0
42	34.18279678608904	36.0	33.0	39.0	24.0	40.0
43	33.99747412720003	36.0	33.0	39.0	24.0	40.0
44	33.8048395017086	36.0	32.0	39.0	23.0	40.0
45	33.704956210076645	36.0	32.0	39.0	23.0	40.0
46	33.55541427109211	35.0	32.0	39.0	23.0	40.0
47	33.24242788297126	35.0	32.0	39.0	23.0	40.0
48	33.101706178406594	35.0	32.0	38.0	23.0	40.0
49	32.832403254670666	35.0	31.0	38.0	22.0	40.0
50	32.50721948409232	35.0	31.0	38.0	21.0	40.0
51	32.334421514465035	35.0	31.0	38.0	21.0	40.0
52	31.728577809312366	34.0	30.0	37.0	19.0	40.0
53	31.710938529958288	34.0	30.0	37.0	20.0	39.0
54	31.7009412994774	34.0	30.0	37.0	20.0	39.0
55	31.66688533378058	34.0	30.0	37.0	20.0	39.0
56	31.525701910532042	34.0	30.0	36.0	20.0	39.0
57	31.247678074428894	34.0	30.0	36.0	19.0	39.0
58	31.040212545227714	34.0	29.0	36.0	18.0	39.0
59	30.20440022465419	33.0	28.0	35.0	17.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	12.0
14	29.0
15	154.0
16	511.0
17	1478.0
18	3253.0
19	6222.0
20	11112.0
21	16927.0
22	24473.0
23	34845.0
24	47717.0
25	62490.0
26	80772.0
27	102290.0
28	124429.0
29	149441.0
30	180389.0
31	221184.0
32	272060.0
33	341511.0
34	447670.0
35	551807.0
36	716515.0
37	858781.0
38	733417.0
39	54703.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.58699055568211	19.913159616882528	30.663473294163566	16.836376533271796
2	29.852997414652915	20.51589182579507	33.32365884996165	16.307451909590366
3	22.646498540798465	21.38106422092555	36.978115644571375	18.99432159370461
4	20.90665769001488	22.228671877803343	38.42274597919154	18.44192445299024
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.25068350819764	0.690218879238856	0.042722359971269955	0.016375252592236187
12	99.38594785255215	0.47234475576588303	0.09795414413830389	0.04375324754366255
13	97.55865998096426	1.6189098086375295	0.32940822890144855	0.4930219814967576
14	62.721578875686255	24.400553745224464	5.36339084298254	7.514476536106739
15	31.870840889045372	27.982233842175475	17.673100396356446	22.473824872422707
16	27.658396371117146	18.10482421681786	29.635123291179944	24.60165612088505
17	22.09812582656863	17.860166841223688	30.42930321714239	29.612404115065296
18	22.258508143119705	18.91387271353597	31.480868015265862	27.346751128078463
19	23.816833482118167	18.613170738573455	30.265550691220028	27.304445088088354
20	27.397423217213763	18.680614575521137	25.923809082000567	27.998153125264537
21	29.03465110502227	18.971047324281745	21.683748671988823	30.310552898707165
22	29.05267181277813	20.83116500009813	23.25065813250355	26.865505054620193
23	30.005846322021128	19.770956606175375	20.66654018469144	29.55665688711206
24	28.027315348706644	21.20767686115352	22.02681616122447	28.738191628915366
25	30.96235139111339	18.99721600881171	22.739813690861002	27.3006189092139
26	30.13589675423065	20.448408339325365	21.180219567158062	28.235475339285916
27	30.01113953321014	21.501420345002387	20.845280229935508	27.642159891851964
28	29.729033184072705	21.304679416763463	20.125998251059585	28.840289148104247
29	28.78743633525812	21.593684782731522	20.59063117479353	29.028247707216824
30	29.556815485200122	20.52039204654378	21.217133272154122	28.705659196101973
31	29.9025791419328	21.100999822171893	21.088311975127063	27.908109060768243
32	30.494487031335215	22.47802772175631	19.452570944394125	27.57491430251435
33	30.55552747047746	23.00312616656328	19.808484878762666	26.63286148419659
34	29.121424083952313	22.278630275542373	20.50683191001462	28.0931137304907
35	29.69178245813952	24.199530668607906	19.990833030510107	26.117853842742463
36	32.10844064972086	22.714953440557537	19.415141795611866	25.76146411410974
37	31.38818725755556	23.036292991728914	20.33469351018606	25.24082624052946
38	30.010247418964802	24.29611690423669	22.04505494135142	23.64858073544709
39	31.07977345056111	22.87577190185078	22.59465679076372	23.449797856824386
40	30.348378542709575	24.825259600289204	20.292050449258824	24.5343114077424
41	32.024720683985976	23.599811823368515	19.660175841665183	24.715291650980326
42	31.311049112475185	24.702722752501536	19.613885024712555	24.37234311031072
43	31.401073352210474	23.306008865236628	19.86534229333232	25.427575489220583
44	30.74739150750853	23.825794274093578	21.360862789458857	24.065951428939037
45	28.641486444720538	24.228157623502806	20.809556010599902	26.32079992117675
46	28.59721775339068	23.19078735426075	21.054649530936242	27.157345361412332
47	28.87383264372902	21.68860573843567	23.596917408261415	25.84064420957389
48	28.639424669575753	21.639698053030045	21.972397192258747	27.74848008513545
49	29.995577095819215	20.932429879324697	22.119596042739804	26.952396982116284
50	27.47186519479711	22.276330603265496	22.485006037630964	27.76679816430643
51	28.40325229169281	22.99981543147502	21.6616242387044	26.935308038127776
52	29.221479652757417	22.56376981311396	22.159186090471884	26.055564443656742
53	26.731608618775198	24.124434473911307	22.36690993630899	26.777046971004502
54	26.364632467764444	23.862311483869483	22.502590600644663	27.27046544772141
55	26.631731472819165	23.76249381219647	21.40582534742398	28.199949367560386
56	28.53936910077065	23.370023018530006	20.445850945155396	27.644756935543953
57	28.915464641844878	22.721892106910175	21.455367425181848	26.9072758260631
58	27.84242962755023	23.213942675117565	21.19782395493277	27.74580374239943
59	29.226713389663413	22.80434328794058	20.88709065090043	27.081852671495582
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	4.0
4	89.5
5	173.0
6	675.5
7	1178.0
8	1685.0
9	2799.5
10	3407.0
11	3966.0
12	4525.0
13	4317.0
14	4177.5
15	4246.0
16	4487.0
17	4728.0
18	5469.0
19	8029.0
20	9848.0
21	12832.0
22	15816.0
23	18785.0
24	21754.0
25	26408.0
26	34983.5
27	38905.0
28	39661.0
29	40417.0
30	42097.0
31	47116.5
32	50456.0
33	51853.5
34	53251.0
35	52793.5
36	58259.5
37	64183.0
38	70386.5
39	76590.0
40	83051.5
41	89513.0
42	104040.5
43	126076.0
44	133584.0
45	143164.5
46	152745.0
47	170424.0
48	206999.0
49	225895.0
50	245111.0
51	264327.0
52	302452.5
53	369467.5
54	398357.0
55	425289.0
56	452221.0
57	453316.0
58	431000.5
59	407590.0
60	381223.0
61	354856.0
62	321531.5
63	288207.0
64	244230.0
65	173454.5
66	146656.0
67	133874.0
68	121092.0
69	95855.5
70	68342.0
71	66065.0
72	46657.5
73	27250.0
74	16972.0
75	5396.5
76	4099.0
77	3115.0
78	2131.0
79	1438.0
80	745.0
81	544.5
82	220.5
83	97.0
84	57.0
85	17.0
86	11.5
87	4.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	5044197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.418253147721146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.43735538466515	11.0616530338009
2	7.533027683903748	2.02160144862824
3	2.9689221043895766	1.1951324511769297
4	1.6156157009646974	0.8671496185990903
5	0.9989465865212422	0.6702059089496976
6	0.6657414119475669	0.5359852077860255
7	0.4600822270095011	0.43214498535465545
8	0.33124529888556187	0.3555786619551218
9	0.22947601189805922	0.27712504970798657
>10	1.287906301063287	3.363826083790954
>50	0.1803903799997202	1.737911368069051
>100	0.8817614659897388	34.95913074914391
>500	0.3443127433201021	30.9912179798861
>1k	0.06521669944219186	11.531337453151396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0020617751447851856	0.0	0.0	0.0	0.0
12	0.008029028208057695	0.0	0.0	0.0	0.0
13	0.016950170661455134	0.0	0.0	0.0	0.0
14	0.06351853426818976	0.0	0.0	0.0	0.0
15	0.09823169079240958	0.0	0.0	0.0	0.0
16	0.11399237579341172	0.0	0.0	0.0	0.0
17	0.12834550276287782	0.0	0.0	0.0	0.0
18	0.1449586524872046	0.0	0.0	0.0	0.0
19	0.16206742123672013	0.0	0.0	0.0	0.0
20	0.18068287182280945	0.0	0.0	0.0	0.0
21	0.20015078713222342	0.0	0.0	0.0	0.0
22	0.22336558227206432	0.0	0.0	0.0	0.0
23	0.24166383668203284	0.0	0.0	0.0	0.0
24	0.26089385485935623	0.0	0.0	0.0	0.0
25	0.2838707528671065	0.0	0.0	0.0	0.0
26	0.3114271706676008	0.0	0.0	0.0	0.0
27	0.33418599630426804	0.0	0.0	0.0	0.0
28	0.3582136066454185	0.0	0.0	0.0	0.0
29	0.3916976279871702	0.0	0.0	0.0	0.0
30	0.42995941673174143	0.0	0.0	0.0	0.0
31	0.46217465336901	0.0	0.0	0.0	0.0
32	0.48368451906220156	0.0	0.0	0.0	0.0
33	0.5030533105665778	0.0	0.0	0.0	0.0
34	0.5216885859136746	0.0	0.0	0.0	0.0
35	0.5407600060029376	0.0	0.0	0.0	0.0
36	0.5597917765701855	0.0	0.0	0.0	0.0
37	0.5782089795461993	0.0	0.0	0.0	0.0
38	0.5975975958115831	0.0	0.0	0.0	0.0
39	0.6195634310079483	0.0	0.0	0.0	0.0
40	0.6418068128584193	0.0	0.0	0.0	0.0
41	0.6605610367715614	0.0	0.0	0.0	0.0
42	0.6810003653703454	0.0	0.0	0.0	0.0
43	0.6994373931073667	0.0	0.0	0.0	0.0
44	0.7197577731401054	0.0	0.0	0.0	0.0
45	0.7391860389275042	0.0	0.0	0.0	0.0
46	0.7578411390356087	0.0	0.0	0.0	0.0
47	0.7749102582631091	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	65	0.0	53.0	32
ACGTTTA	165	0.0	53.0	29
CCGATTA	50	3.6379788E-12	53.0	19
ACGTTAA	20	3.1392663E-4	53.0	25
CCGATAC	50	3.6379788E-12	53.0	42
CGCATAA	30	7.01526E-7	53.0	33
CATTACG	55	0.0	53.0	16
TTATACG	55	0.0	53.0	47
CGGATTA	50	3.6379788E-12	53.0	42
ACTACGT	25	1.4794534E-5	53.0	39
TTAGTAC	20	3.1392663E-4	53.0	50
TTACGCG	40	1.6025297E-9	53.0	25
TACTACG	20	3.1392663E-4	53.0	15
ACCGTTC	15	0.0066975756	53.0	24
TTACACG	50	3.6379788E-12	53.0	45
CACGATA	85	0.0	52.999996	20
CGTATAT	1755	0.0	52.54701	1
CGATATT	4060	0.0	52.543102	2
CGTTTAT	3055	0.0	52.306053	1
CCGTTAT	1450	0.0	51.172413	1
>>END_MODULE