FastQCFastQC Report
Fri 17 Jun 2016
SRR1528068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3397447
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG2900.046.00000427
GCTATAG750.046.00000446
TACTGAC1500.046.00000417
CTGTTTA1450.046.00000432
GACATTT1450.046.00000421
TTAGTAG750.046.00000437
CTACAAT750.046.00000437
CTTAATA501.6370905E-1146.034
ATTTAGT1200.046.044
ATTTACC1200.046.045
TATTTAG1050.046.043
CTTAAAT600.046.026
CTAGATG253.4185774E-546.031
GTCATAA253.4185774E-546.014
TAATATG1750.046.022
TAATAAT800.046.036
CCTTAAA600.046.025
CAAACTG650.046.025
CTCACCG551.8189894E-1246.026
GATGTTA206.3132064E-446.031
Produced by FastQC (version 0.11.5)