FastQCFastQC Report
Fri 17 Jun 2016
SRR1528067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3380127
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATG206.3132006E-446.00000431
TAGACCG206.3132006E-446.00000416
TTTCGGG206.3132006E-446.00000443
CGATTAA800.046.00000416
TGACGAG206.3132006E-446.00000436
TGTGCAC405.6170393E-946.00000421
TGTTGTT405.6170393E-946.00000426
ATTCCGA206.3132006E-446.00000430
ACCTAAT206.3132006E-446.00000423
TACTGAG206.3132006E-446.00000437
TATAGAG206.3132006E-446.00000423
TAGGCTG405.6170393E-946.00000421
GATTCCG206.3132006E-446.00000429
TGCACAC206.3132006E-446.00000418
CTTGTGT253.418573E-546.044
CTTAATG453.110472E-1046.041
ATCCTTA1700.046.029
ATTTAGT1800.046.044
ATTTAGG453.110472E-1046.030
CTTAAAT501.6370905E-1146.026
Produced by FastQC (version 0.11.5)