##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528067_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3380127
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.806879741500836	31.0	31.0	34.0	27.0	34.0
2	31.539399555105472	31.0	31.0	34.0	30.0	34.0
3	31.72118799086543	31.0	31.0	34.0	30.0	34.0
4	35.49905787563603	37.0	35.0	37.0	33.0	37.0
5	35.48153190693723	37.0	35.0	37.0	33.0	37.0
6	35.45184396917631	37.0	35.0	37.0	33.0	37.0
7	35.366185057543696	37.0	35.0	37.0	33.0	37.0
8	35.404088958787646	37.0	35.0	37.0	33.0	37.0
9	37.22135115041535	39.0	37.0	39.0	34.0	39.0
10	37.3804590774252	39.0	37.0	39.0	34.0	39.0
11	37.773354078116	39.0	37.0	39.0	35.0	39.0
12	37.674571103393454	39.0	37.0	39.0	35.0	39.0
13	37.50190273915744	39.0	37.0	39.0	35.0	39.0
14	37.8090397195135	40.0	38.0	40.0	33.0	40.0
15	36.90644641458738	39.0	36.0	40.0	31.0	40.0
16	36.39470410431324	38.0	35.0	40.0	31.0	40.0
17	36.56205107086213	38.0	36.0	40.0	31.0	40.0
18	35.95482063247919	38.0	34.0	40.0	29.0	40.0
19	36.71433055621875	39.0	36.0	40.0	31.0	40.0
20	36.96101329920444	39.0	36.0	40.0	32.0	40.0
21	37.09021968701176	39.0	36.0	40.0	32.0	40.0
22	37.18861598987257	39.0	36.0	40.0	32.0	40.0
23	37.184483896610985	39.0	36.0	40.0	32.0	40.0
24	37.325023882238746	40.0	37.0	40.0	32.0	40.0
25	37.47093585536875	40.0	37.0	40.0	32.0	40.0
26	37.096956120287786	40.0	36.0	40.0	31.0	40.0
27	36.746589699144444	39.0	36.0	40.0	31.0	40.0
28	36.539559904110114	39.0	36.0	40.0	30.0	40.0
29	36.36860745173184	38.0	35.0	40.0	30.0	40.0
30	35.80386890788423	38.0	35.0	40.0	29.0	40.0
31	35.73113495439669	38.0	35.0	40.0	29.0	40.0
32	35.610138908981824	38.0	35.0	40.0	29.0	40.0
33	35.56939901962264	38.0	35.0	40.0	29.0	40.0
34	35.32976512420983	38.0	35.0	40.0	27.0	40.0
35	35.18282389981205	38.0	34.0	40.0	25.0	40.0
36	34.94138119662368	38.0	34.0	40.0	25.0	40.0
37	34.77101836706136	38.0	34.0	40.0	24.0	40.0
38	34.533691189709735	38.0	33.0	40.0	22.0	40.0
39	34.12981731159805	38.0	33.0	40.0	20.0	40.0
40	33.65392720451036	38.0	33.0	40.0	15.0	40.0
41	33.15655772697298	37.0	32.0	40.0	15.0	40.0
42	32.46373612589113	37.0	31.0	40.0	10.0	40.0
43	31.725742257613398	35.0	30.0	39.0	10.0	40.0
44	31.000349691002736	35.0	29.0	39.0	9.0	40.0
45	30.519800291527506	35.0	27.0	39.0	9.0	40.0
46	30.17124622832219	35.0	26.0	38.0	9.0	40.0
47	29.77317420321781	35.0	24.0	38.0	8.0	40.0
48	29.239864655972987	35.0	23.0	38.0	8.0	40.0
49	28.713404555509303	34.0	20.0	38.0	8.0	40.0
50	28.30636274909197	34.0	20.0	37.0	8.0	40.0
51	27.918006335264916	33.0	18.0	37.0	8.0	40.0
52	27.009479525473452	33.0	15.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	6.0
14	15.0
15	27.0
16	130.0
17	429.0
18	1132.0
19	2640.0
20	5281.0
21	9896.0
22	16003.0
23	24447.0
24	35354.0
25	49031.0
26	66943.0
27	87446.0
28	114017.0
29	143421.0
30	169848.0
31	175467.0
32	165143.0
33	172141.0
34	205534.0
35	263055.0
36	347350.0
37	527226.0
38	685894.0
39	112247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.637347945802034	24.713450115927596	39.16625026219429	6.482951676076077
2	19.065555820831584	22.43835808536188	39.120749013276715	19.375337080529814
3	22.601310542473698	23.562102844064736	33.586903687346656	20.249682926114907
4	26.57361690847711	27.771648816745643	35.972553693988424	9.682180580788827
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.21860924160542	0.5993561780370974	0.1049073008203538	0.07712727953712982
12	98.88924291897908	0.8193183273882904	0.1748750860544589	0.11656366757817088
13	93.60760705145103	5.2747426354098526	0.6997370217154563	0.41791329142366546
14	41.01011589209518	50.14699151836602	5.4671318562882405	3.3757607332505555
15	41.07804233391231	42.725228963290434	11.185289783490383	5.011438919306878
16	27.13989740622172	58.69214381589805	8.131055430757483	6.036903347122756
17	40.7501552456461	29.430787659753616	14.908848099494488	14.910208995105807
18	12.426041980079447	35.98687268259447	39.802676053296224	11.784409284029861
19	21.276271572044482	38.86540357803124	19.446961608247264	20.411363241677012
20	22.587967848545336	33.476523219393826	24.515173542295894	19.42033538976494
21	25.79317286007301	38.049102888737615	19.807421437123516	16.350302814065863
22	24.660907711455813	40.08106795987251	17.73045805675349	17.527566271918186
23	9.108503911243572	31.119984544959404	20.785875796974494	38.98563574682253
24	11.537229222452293	36.7711331556477	35.0245419772689	16.667095644631104
25	13.073118258574306	28.14409044393894	14.718086036412242	44.06470526107451
26	11.996738584082788	35.606324851107665	6.788147309257907	45.60878925555164
27	12.460981495665695	64.44530042806083	12.395717675696801	10.698000400576664
28	16.242525798586858	38.512665352514865	37.345016918003374	7.899791930894905
29	45.16081792193015	32.42830816711916	9.825843821844565	12.58503008910612
30	50.353965990035285	32.28491118824825	9.454408074016154	7.906714747700308
31	40.77130829699594	34.562991272221424	11.630983096197273	13.034717334585357
32	25.819148215436876	54.56945848484391	11.200703405522928	8.410689894196283
33	22.278216173534307	55.26168099600991	10.92621667765738	11.5338861527984
34	14.731665407838227	62.9641430632636	11.768285629504453	10.535905899393722
35	18.58152075351015	55.572142703513805	12.120668838774401	13.725667704201646
36	11.150320683216933	56.180433457086075	18.321560107061067	14.347685752635922
37	12.4976960924841	59.21348517378193	14.89973601583609	13.389082717897876
38	13.496475132443248	55.928786107740926	15.674174372738065	14.900564387077765
39	16.26503974554802	55.91266245321551	13.720519968628398	14.101777832608065
40	16.74942982911589	55.950767530332435	14.012136230384243	13.287666410167429
41	16.157706500377056	51.88423393558881	13.641854285356732	18.3162052786774
42	17.847051309018862	51.66563268184894	17.373430051592738	13.113885957539464
43	18.24250982285577	50.740992868019454	11.215406995062612	19.801090314062165
44	17.80338431070785	51.5392173134323	11.1339011818195	19.523497194040342
45	17.58528599665042	57.08859460014373	13.947996628529047	11.37812277467681
46	18.466613828415323	51.67643109267787	19.208716122204876	10.648238956701924
47	25.830952505630705	51.4853140133492	12.119929221594337	10.563804259425755
48	26.34365513485144	51.9150020102795	11.276085188515106	10.465257666353956
49	23.840021395645785	53.45145315545836	11.964402520970365	10.74412292792549
50	19.709052352174933	57.2258971334509	12.297407760122622	10.767642754251542
51	18.414456024877172	57.69694452309041	12.083392132899148	11.805207319133276
52	21.04255254314409	55.0094715376079	11.639237223926793	12.308738695321212
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	6.5
6	13.0
7	30.0
8	47.0
9	81.0
10	115.0
11	752.0
12	1389.0
13	3892.5
14	11150.0
15	15904.0
16	18655.5
17	21407.0
18	21830.0
19	22253.0
20	25052.5
21	27852.0
22	34975.0
23	42098.0
24	52106.0
25	62114.0
26	76955.5
27	91797.0
28	111455.0
29	131113.0
30	160865.5
31	190618.0
32	220791.0
33	250964.0
34	259452.0
35	267940.0
36	244723.0
37	221506.0
38	189366.5
39	138246.5
40	119266.0
41	116060.5
42	112855.0
43	114666.5
44	116478.0
45	126708.0
46	136938.0
47	158760.0
48	180582.0
49	217286.5
50	253991.0
51	266457.0
52	278923.0
53	275839.0
54	272755.0
55	233236.0
56	193717.0
57	152652.0
58	111587.0
59	82105.5
60	52624.0
61	36765.0
62	20906.0
63	15202.5
64	7745.5
65	5992.0
66	3609.0
67	1226.0
68	1172.5
69	1119.0
70	751.0
71	383.0
72	332.5
73	282.0
74	205.0
75	128.0
76	91.0
77	54.0
78	40.5
79	27.0
80	17.5
81	8.0
82	18.0
83	28.0
84	16.5
85	5.0
86	3.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3380127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.45423208026297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.57569618615233	36.75171930422875
2	6.828526792430342	5.934567760091235
3	2.0907239800572053	2.7255241513553066
4	1.0241478017260233	1.7801422504277506
5	0.6596697379783644	1.4332720945219053
6	0.46650313450739245	1.2162921283832617
7	0.3476247415017527	1.0574036335841006
8	0.27849467195984523	0.9681417686767854
9	0.23131421522002074	0.9046423432471209
>10	3.141186637713813	30.606338541901785
>50	0.27761351816188046	7.9251505316077715
>100	0.07095195210379157	5.4820990332689075
>500	0.004569519377971262	1.3646226141287565
>1k	0.0029771111093742043	1.850083844576584
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.026774141918336204	0.0	0.0	0.0	0.0
12	0.0411227152115882	0.0	0.0	0.0	0.0
13	0.11665242163977863	0.0	0.0	0.0	0.0
14	0.7347061219889075	0.0	0.0	0.0	0.0
15	1.1970556135908503	0.0	0.0	0.0	0.0
16	1.6217733830711094	0.0	0.0	0.0	0.0
17	1.9528556175551983	0.0	0.0	0.0	0.0
18	2.370413892732433	0.0	0.0	0.0	0.0
19	2.7109928118085502	0.0	0.0	0.0	0.0
20	2.9356293417377515	0.0	0.0	0.0	0.0
21	3.2125420139539136	0.0	0.0	0.0	0.0
22	3.384902401596153	0.0	0.0	0.0	0.0
23	3.5612271373235385	0.0	0.0	0.0	0.0
24	3.8260100877866425	0.0	0.0	0.0	0.0
25	4.020825253015641	0.0	0.0	0.0	0.0
26	4.224841255964643	0.0	0.0	0.0	0.0
27	4.50352900941296	0.0	0.0	0.0	0.0
28	4.7663297858334905	0.0	0.0	0.0	0.0
29	4.97306758000513	0.0	0.0	0.0	0.0
30	5.210159263246617	0.0	0.0	0.0	0.0
31	5.4380205240809	0.0	0.0	0.0	0.0
32	5.981727905489942	0.0	0.0	0.0	0.0
33	6.301449620088239	0.0	0.0	0.0	0.0
34	6.637531666709564	0.0	0.0	0.0	0.0
35	6.875422136505522	0.0	0.0	0.0	0.0
36	7.134850258584959	0.0	0.0	0.0	0.0
37	7.376675491778859	0.0	0.0	0.0	0.0
38	7.601844546077706	0.0	0.0	0.0	0.0
39	7.837959934641509	0.0	0.0	0.0	0.0
40	8.07715213067438	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATG	20	6.3132006E-4	46.000004	31
TAGACCG	20	6.3132006E-4	46.000004	16
TTTCGGG	20	6.3132006E-4	46.000004	43
CGATTAA	80	0.0	46.000004	16
TGACGAG	20	6.3132006E-4	46.000004	36
TGTGCAC	40	5.6170393E-9	46.000004	21
TGTTGTT	40	5.6170393E-9	46.000004	26
ATTCCGA	20	6.3132006E-4	46.000004	30
ACCTAAT	20	6.3132006E-4	46.000004	23
TACTGAG	20	6.3132006E-4	46.000004	37
TATAGAG	20	6.3132006E-4	46.000004	23
TAGGCTG	40	5.6170393E-9	46.000004	21
GATTCCG	20	6.3132006E-4	46.000004	29
TGCACAC	20	6.3132006E-4	46.000004	18
CTTGTGT	25	3.418573E-5	46.0	44
CTTAATG	45	3.110472E-10	46.0	41
ATCCTTA	170	0.0	46.0	29
ATTTAGT	180	0.0	46.0	44
ATTTAGG	45	3.110472E-10	46.0	30
CTTAAAT	50	1.6370905E-11	46.0	26
>>END_MODULE