FastQCFastQC Report
Fri 17 Jun 2016
SRR1528066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235799
Sequences flagged as poor quality0
Sequence length59
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATT203.139097E-453.00000441
TTAGGTG800.053.00000452
GTATTAG203.139097E-453.00000431
GCTATAG401.6025297E-953.00000446
CGATTAA800.053.00000416
TAGCCAC1600.053.00000445
TCGTGTA203.139097E-453.00000445
TCAATAG203.139097E-453.00000442
CGTCAGT1950.053.00000443
TTTAGCA150.006697360453.028
CGGGTCA353.3445758E-853.023
GATGTTA307.014478E-753.031
ATGATTA1050.053.039
CGACGTT457.6397555E-1153.031
GCAACTT150.006697360453.052
CTTCAAT2350.053.053
TTATGCA150.006697360453.044
CGGCAAT251.4793348E-553.028
TAACGTG150.006697360453.030
CTGACTA700.053.018