##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528066_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3235799
Sequences flagged as poor quality	0
Sequence length	59
%GC	40
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.3482747228737	31.0	30.0	33.0	27.0	34.0
2	31.1388908890818	31.0	31.0	34.0	28.0	34.0
3	31.36210531000226	31.0	31.0	34.0	30.0	34.0
4	35.20019506774061	35.0	35.0	37.0	33.0	37.0
5	34.971556329673135	35.0	35.0	37.0	32.0	37.0
6	35.079164064269754	36.0	35.0	37.0	32.0	37.0
7	34.85282367662516	36.0	35.0	37.0	32.0	37.0
8	35.02094969434133	37.0	35.0	37.0	32.0	37.0
9	36.83131492407285	39.0	37.0	39.0	33.0	39.0
10	37.012671677072646	39.0	37.0	39.0	33.0	39.0
11	37.40133549704416	39.0	37.0	39.0	35.0	39.0
12	37.334970744474546	39.0	37.0	39.0	35.0	39.0
13	37.16670782085043	39.0	37.0	39.0	34.0	39.0
14	37.1481365189865	40.0	36.0	40.0	32.0	40.0
15	36.15163086458708	38.0	35.0	40.0	30.0	40.0
16	35.488360989047834	38.0	34.0	40.0	27.0	40.0
17	35.890890627013604	38.0	34.0	40.0	29.0	40.0
18	35.346771848313196	38.0	34.0	40.0	27.0	40.0
19	36.17087340715539	38.0	34.0	40.0	30.0	40.0
20	36.23162470845686	38.0	35.0	40.0	30.0	40.0
21	36.30732811277833	38.0	35.0	40.0	30.0	40.0
22	36.381295624357385	39.0	35.0	40.0	30.0	40.0
23	36.270308508037736	39.0	35.0	40.0	30.0	40.0
24	36.39337393948141	39.0	35.0	40.0	30.0	40.0
25	36.583377088626335	39.0	36.0	40.0	30.0	40.0
26	36.17536719678818	39.0	35.0	40.0	30.0	40.0
27	35.82642308746619	38.0	35.0	40.0	27.0	40.0
28	35.596541997818775	38.0	34.0	40.0	27.0	40.0
29	35.29932854296574	37.0	34.0	40.0	27.0	40.0
30	34.66782238328153	37.0	33.0	40.0	25.0	40.0
31	34.61857705005781	37.0	33.0	40.0	25.0	40.0
32	34.36229598933679	37.0	33.0	40.0	25.0	40.0
33	34.10476423288344	37.0	33.0	40.0	24.0	40.0
34	33.79439421299036	36.0	33.0	40.0	22.0	40.0
35	33.87927680303999	37.0	33.0	40.0	22.0	40.0
36	33.43387429194458	36.0	32.0	40.0	20.0	40.0
37	33.415158049062995	37.0	32.0	40.0	20.0	40.0
38	33.299335959990096	37.0	32.0	40.0	18.0	40.0
39	32.80510254190696	36.0	31.0	40.0	15.0	40.0
40	32.404574882432435	36.0	31.0	40.0	15.0	40.0
41	31.91242997479139	36.0	30.0	40.0	12.0	40.0
42	31.32546242829051	35.0	28.0	39.0	10.0	40.0
43	30.66267527741989	35.0	27.0	38.0	10.0	40.0
44	30.045172150680557	35.0	26.0	38.0	9.0	40.0
45	29.626772861973194	34.0	24.0	38.0	9.0	40.0
46	29.21699957259397	34.0	23.0	37.0	8.0	40.0
47	28.634205956550453	33.0	22.0	37.0	8.0	40.0
48	28.066409254715758	33.0	20.0	37.0	8.0	40.0
49	27.62022548372133	33.0	19.0	36.0	8.0	40.0
50	27.00416713151837	33.0	17.0	35.0	8.0	39.0
51	26.61038896420946	33.0	15.0	35.0	8.0	39.0
52	25.904331202278016	31.0	12.0	35.0	8.0	39.0
53	25.678627442557463	31.0	12.0	35.0	8.0	39.0
54	25.31177091036866	31.0	11.0	35.0	8.0	38.0
55	24.850699317232003	30.0	10.0	35.0	8.0	38.0
56	24.046970779087328	28.0	10.0	35.0	8.0	38.0
57	23.200445083270004	26.0	9.0	35.0	8.0	38.0
58	22.364857026039008	23.0	9.0	34.0	8.0	37.0
59	21.282795377586805	22.0	8.0	33.0	8.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	10.0
14	39.0
15	172.0
16	600.0
17	1919.0
18	4761.0
19	10097.0
20	18616.0
21	29995.0
22	44143.0
23	62123.0
24	82700.0
25	107894.0
26	134606.0
27	160419.0
28	169337.0
29	156507.0
30	152716.0
31	169833.0
32	197176.0
33	228698.0
34	263036.0
35	293669.0
36	325725.0
37	350968.0
38	252869.0
39	17168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.91323008629399	25.424972317501798	38.26597387538595	6.3958237208182585
2	18.678910525653787	23.119853859896736	38.78080807862293	19.420427535826544
3	22.37917126496423	24.002479758476962	33.39008387109335	20.22826510546545
4	26.491509515887728	28.163955795771002	35.7520970863765	9.59243760196477
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.21144669369141	0.6193833424140375	0.09373264532191276	0.07543731857263075
12	98.88287251464013	0.8338280591594225	0.1597132578383268	0.12358616836212633
13	93.65538465151883	5.259659206273319	0.6735276202261018	0.41142852198174235
14	40.3028432853833	50.74239778181525	5.5593997031336	3.39535922966785
15	41.068712858864224	42.78955522268225	11.226624397868965	4.915107520584561
16	27.28828335752623	58.46651785231407	8.235431187165828	6.009767602993882
17	40.89110602976266	28.984989487913186	15.0593099262346	15.064594556089547
18	12.144821109098556	35.90315714913071	40.08781138754292	11.864210354227813
19	21.453928380594718	38.40266345344689	19.574825259541768	20.568582906416623
20	22.54243851364068	33.048189952466146	25.001645652279393	19.407725881613782
21	25.988604360159577	37.573254704634	20.03947093129085	16.398670003915573
22	24.771532471578116	39.73618262444608	17.98650039758341	17.505784506392395
23	8.786485192683477	30.596492550989723	21.22063206027321	39.39639019605359
24	11.029918731046026	36.73352393025648	35.54568747935208	16.690869859345405
25	12.696029635956993	27.69968097523981	15.15882166970198	44.44546771910122
26	11.642441325929083	35.269279704950776	6.670315430593804	46.417963538526344
27	12.221000130106969	64.98163204822055	12.336952944234175	10.460414877438309
28	15.941255930915363	38.64621999079671	37.662042667050706	7.7504814112372244
29	45.39101470765026	32.16516229839987	9.607178937875931	12.83664405607394
30	50.70262398869646	32.10122136758186	9.425307319768626	7.770847323953063
31	40.83350047391695	34.52967875940378	11.4620840169615	13.174736749717766
32	25.68181769015937	55.216408682986795	10.951947262484474	8.149826364369357
33	22.3602578528518	55.667549189551025	10.774494954723703	11.197698002873478
34	14.383526294432997	63.845096682457715	11.666732080700934	10.10464494240835
35	18.740162785142093	56.165633279446595	11.691022835472785	13.403181099938532
36	10.84894333671529	56.62944453595542	18.444810694360186	14.076801432969106
37	12.122322801879845	60.03846345214892	14.693434295517118	13.145779450454123
38	13.118027417648623	56.85594191728225	15.38772958394511	14.638301081124014
39	15.862357334309085	56.924982052346266	13.422681693145957	13.78997892019869
40	16.34653450353375	57.06874870781529	13.634097791611902	12.950618997039061
41	15.431088272170182	52.802661722807876	13.236112626278704	18.53013737874324
42	17.047505113883773	52.795213794181905	17.329413848017136	12.82786724391719
43	17.305555752999492	51.907488691355674	10.722204933001093	20.064750622643743
44	16.89310739016855	52.90007197604054	10.426080235515247	19.780740398275665
45	16.642659201019594	59.10521636232659	13.208515114813993	11.04360932183983
46	17.585641135311555	52.99680233537374	19.076648456841728	10.340908072472981
47	25.424446944943117	52.55159544829577	11.696647412277462	10.32731019448365
48	25.96147041271723	53.06587337470591	10.689260983145122	10.283395229431742
49	23.733396295628992	54.4582342722771	11.27795638727869	10.530413044815207
50	18.810469995200567	58.72781343958633	11.802679956326088	10.659036608887016
51	17.68407122939342	59.10645253305289	11.634406216208113	11.575070021345578
52	19.234043894568234	56.91360928166428	11.68675804646704	12.165588777300444
53	16.768346859616436	56.09167936574553	11.322551246230066	15.817422528407976
54	16.147171069649257	58.20046300774554	12.43918426329942	13.213181659305784
55	18.858093472431385	54.644339775122	12.820944687849895	13.67662206459672
56	17.526243131912704	51.826859455732574	15.768902827400588	14.877994584954132
57	20.631998464675956	49.98079299734007	14.14380188633472	15.243406651649252
58	21.2019967865742	47.35593898137678	15.07522562433575	16.366838607713273
59	20.95754402544781	43.52974334932423	18.24717789949252	17.26553472573544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	4.0
5	8.0
6	24.0
7	40.0
8	59.0
9	504.0
10	930.0
11	2597.0
12	4264.0
13	7437.0
14	11750.5
15	12891.0
16	11892.0
17	10893.0
18	10424.0
19	10859.0
20	11763.0
21	13940.0
22	16117.0
23	22549.0
24	28981.0
25	41657.0
26	80433.0
27	106533.0
28	141020.0
29	175507.0
30	201716.0
31	230502.5
32	233080.0
33	219766.5
34	206453.0
35	190412.0
36	162640.5
37	150910.0
38	146005.5
39	141101.0
40	136748.5
41	132396.0
42	128293.0
43	129450.0
44	134710.0
45	136223.0
46	137736.0
47	146720.5
48	158206.5
49	160708.0
50	177127.5
51	193547.0
52	190859.0
53	179919.0
54	171667.0
55	145462.5
56	119258.0
57	96051.0
58	53810.5
59	34777.0
60	25101.0
61	15425.0
62	11307.5
63	7190.0
64	5374.5
65	3904.5
66	4250.0
67	2861.5
68	1473.0
69	1020.5
70	506.0
71	444.0
72	328.5
73	213.0
74	162.0
75	74.5
76	38.0
77	32.5
78	27.0
79	19.5
80	12.0
81	11.5
82	8.5
83	6.0
84	11.5
85	17.0
86	9.5
87	1.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	3235799.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.72544934410092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.99867014431801	54.65209684885232
2	4.64794687341431	5.644973248311858
3	1.4278860218619767	2.6012706086918778
4	0.7370861386660251	1.7903954790321066
5	0.4489107152293348	1.3630152448841544
6	0.3190521647186092	1.1624751640047388
7	0.2581633196839438	1.0973958514380584
8	0.2063212353895768	1.0023159782609656
9	0.1795157236498766	0.9811055684673182
>10	1.6438285726439836	20.28580858865733
>50	0.1024809858167529	4.049110727243554
>100	0.02585556320831552	2.8584986204704528
>500	0.0025068535024048363	1.0713172552187047
>1k	0.0017756878968751074	1.4402208164665442
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.025434212693680913	0.0	0.0	0.0	0.0
12	0.03866123946512129	0.0	0.0	0.0	0.0
13	0.10216951052892964	0.0	0.0	0.0	0.0
14	0.655572240426553	0.0	0.0	0.0	0.0
15	1.044904210675632	0.0	0.0	0.0	0.0
16	1.4114597352925815	0.0	0.0	0.0	0.0
17	1.6938629377164651	0.0	0.0	0.0	0.0
18	2.057853408076336	0.0	0.0	0.0	0.0
19	2.3550906592158536	0.0	0.0	0.0	0.0
20	2.543359460831776	0.0	0.0	0.0	0.0
21	2.774708812259352	0.0	0.0	0.0	0.0
22	2.928241216466165	0.0	0.0	0.0	0.0
23	3.079332183488529	0.0	0.0	0.0	0.0
24	3.3050878623795854	0.0	0.0	0.0	0.0
25	3.4649247372905423	0.0	0.0	0.0	0.0
26	3.63913209689477	0.0	0.0	0.0	0.0
27	3.875549748300188	0.0	0.0	0.0	0.0
28	4.099760213783365	0.0	0.0	0.0	0.0
29	4.276748957521774	0.0	0.0	0.0	0.0
30	4.480655318825428	0.0	0.0	0.0	0.0
31	4.677762741134416	0.0	0.0	0.0	0.0
32	5.142686551296913	0.0	0.0	0.0	0.0
33	5.419403368379804	0.0	0.0	0.0	0.0
34	5.712653968926994	0.0	0.0	0.0	0.0
35	5.921381396063229	0.0	0.0	0.0	0.0
36	6.145530053010091	0.0	0.0	0.0	0.0
37	6.361025514872834	0.0	0.0	0.0	0.0
38	6.5637266097183415	0.0	0.0	0.0	0.0
39	6.775946219156381	0.0	0.0	0.0	0.0
40	6.986496998113912	0.0	0.0	0.0	0.0
41	7.2211840105025065	0.0	0.0	0.0	0.0
42	7.489154919696804	0.0	0.0	0.0	0.0
43	7.750975879527746	0.0	0.0	0.0	0.0
44	8.03235924110243	0.0	0.0	0.0	0.0
45	8.335715537337146	0.0	0.0	0.0	0.0
46	8.627235498867513	0.0	0.0	0.0	0.0
47	8.904570401313554	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATT	20	3.139097E-4	53.000004	41
TTAGGTG	80	0.0	53.000004	52
GTATTAG	20	3.139097E-4	53.000004	31
GCTATAG	40	1.6025297E-9	53.000004	46
CGATTAA	80	0.0	53.000004	16
TAGCCAC	160	0.0	53.000004	45
TCGTGTA	20	3.139097E-4	53.000004	45
TCAATAG	20	3.139097E-4	53.000004	42
CGTCAGT	195	0.0	53.000004	43
TTTAGCA	15	0.0066973604	53.0	28
CGGGTCA	35	3.3445758E-8	53.0	23
GATGTTA	30	7.014478E-7	53.0	31
ATGATTA	105	0.0	53.0	39
CGACGTT	45	7.6397555E-11	53.0	31
GCAACTT	15	0.0066973604	53.0	52
CTTCAAT	235	0.0	53.0	53
TTATGCA	15	0.0066973604	53.0	44
CGGCAAT	25	1.4793348E-5	53.0	28
TAACGTG	15	0.0066973604	53.0	30
CTGACTA	70	0.0	53.0	18
>>END_MODULE