FastQCFastQC Report
Fri 17 Jun 2016
SRR1528065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5352556
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGTA206.3135324E-446.00000428
AGCGTAA206.3135324E-446.00000440
AATTTCC800.046.00000433
TTAATAC405.6188583E-946.00000446
ACGTTAT206.3135324E-446.00000414
CCGATAT206.3135324E-446.00000425
CGATTTC800.046.00000440
TCGATAA3200.046.00000436
GTCAGTA206.3135324E-446.00000443
AATGCGT206.3135324E-446.00000446
TCGGATA206.3135324E-446.00000443
ACCTAAC206.3135324E-446.00000424
TGACATA405.6188583E-946.00000441
TGATTGA405.6188583E-946.00000423
CGTAGAC405.6188583E-946.00000416
TCATGTA405.6188583E-946.00000430
ACTACGT206.3135324E-446.00000423
CTCATAT405.6188583E-946.00000446
AATTCGT206.3135324E-446.00000419
CTATTAG800.046.00000434