##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5352556
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49475969985181	31.0	31.0	34.0	30.0	34.0
2	31.959813591861533	31.0	31.0	34.0	30.0	34.0
3	32.097605517812426	33.0	31.0	34.0	30.0	34.0
4	35.804464820171894	37.0	35.0	37.0	35.0	37.0
5	35.62370818726605	37.0	35.0	37.0	33.0	37.0
6	35.84370046758969	37.0	35.0	37.0	35.0	37.0
7	35.82133264929877	37.0	35.0	37.0	33.0	37.0
8	35.89866673043682	37.0	35.0	37.0	35.0	37.0
9	37.492319930889096	39.0	37.0	39.0	34.0	39.0
10	37.67474455194864	39.0	37.0	39.0	35.0	39.0
11	37.3115872117919	39.0	37.0	39.0	34.0	39.0
12	37.18480890251312	39.0	37.0	39.0	34.0	39.0
13	37.250825773705124	39.0	37.0	39.0	35.0	39.0
14	37.962912111522044	40.0	38.0	40.0	33.0	40.0
15	37.305224830903214	39.0	37.0	40.0	32.0	40.0
16	36.909273812361796	39.0	36.0	40.0	31.0	40.0
17	36.787497038797916	39.0	36.0	40.0	31.0	40.0
18	37.02804884245956	39.0	36.0	40.0	32.0	40.0
19	37.11764528946544	39.0	36.0	40.0	32.0	40.0
20	37.220560606932466	39.0	36.0	40.0	32.0	40.0
21	37.07065390815154	39.0	36.0	40.0	32.0	40.0
22	37.13365147417421	39.0	36.0	40.0	32.0	40.0
23	37.10170486773048	39.0	36.0	40.0	32.0	40.0
24	36.989723040730446	39.0	36.0	40.0	31.0	40.0
25	37.065759050442445	39.0	36.0	40.0	31.0	40.0
26	36.913894034924624	39.0	35.0	40.0	31.0	40.0
27	36.74722076705036	39.0	35.0	40.0	31.0	40.0
28	36.70188990082495	39.0	35.0	40.0	31.0	40.0
29	36.76644597459606	39.0	35.0	40.0	31.0	40.0
30	36.75854395544857	39.0	35.0	40.0	31.0	40.0
31	36.75769632302773	39.0	35.0	40.0	31.0	40.0
32	36.72544649696332	39.0	35.0	40.0	31.0	40.0
33	36.61368325712052	39.0	35.0	40.0	31.0	40.0
34	36.53629985375211	39.0	35.0	40.0	31.0	40.0
35	36.45483036515638	39.0	35.0	40.0	31.0	40.0
36	36.43444645885069	39.0	35.0	40.0	31.0	40.0
37	36.28886087319778	38.0	35.0	40.0	31.0	40.0
38	36.067975001102276	38.0	35.0	40.0	30.0	40.0
39	35.869636674515874	38.0	35.0	40.0	30.0	40.0
40	35.809372382091844	38.0	35.0	40.0	30.0	40.0
41	35.67431148782003	38.0	34.0	40.0	29.0	40.0
42	35.507840366359545	37.0	34.0	40.0	29.0	40.0
43	35.308564543743216	37.0	34.0	40.0	29.0	40.0
44	35.06763292154253	37.0	34.0	40.0	28.0	40.0
45	34.939680033240194	36.0	34.0	40.0	28.0	40.0
46	34.79964151706213	36.0	33.0	40.0	28.0	40.0
47	34.522237600129735	36.0	33.0	39.0	27.0	40.0
48	34.383342649754624	35.0	33.0	39.0	27.0	40.0
49	34.29141927707062	35.0	33.0	39.0	27.0	40.0
50	34.14140085596489	35.0	33.0	39.0	27.0	40.0
51	33.9883717237148	35.0	33.0	38.0	27.0	40.0
52	33.17858813621007	35.0	32.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	8.0
14	19.0
15	37.0
16	149.0
17	387.0
18	888.0
19	1914.0
20	3737.0
21	6282.0
22	10662.0
23	16030.0
24	23496.0
25	32940.0
26	44865.0
27	60243.0
28	78142.0
29	100741.0
30	127896.0
31	158323.0
32	191389.0
33	241724.0
34	342713.0
35	533802.0
36	567779.0
37	972351.0
38	1488705.0
39	347332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.4721916034134	22.311658205911343	29.94999024764991	19.26615994302535
2	25.23542023661219	20.872850279380543	34.957373636072184	18.93435584793508
3	19.189112640764524	22.237357255113256	36.32152564120767	22.25200446291454
4	19.000623253638075	21.51822419046153	37.39163121319982	22.08952134270057
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.49213048868614	0.43218604345288497	0.048500193178735544	0.02718327468222658
12	99.61156875332084	0.30262177546577745	0.05634691164370817	0.029462559569671014
13	99.18362367437165	0.5148755099432869	0.12437048766981607	0.17713032801525103
14	70.8541676163687	17.823241830631943	3.886348877059857	7.436241675939495
15	36.21256835052263	24.233300875320126	15.256598903402413	24.29753187075483
16	27.519002136549343	16.985062837268774	28.406727552219913	27.089207473961974
17	22.887084226676002	16.62994277873973	29.547322811755727	30.93565018282854
18	24.141550317268983	15.893191962867833	32.07654436497255	27.888713354890637
19	23.95289278617543	15.75251151038868	30.028681624255775	30.265914079180117
20	29.748273535111075	17.341453316882628	22.983468085154083	29.926805062852214
21	31.46890943317548	19.256388910270157	19.981780667030854	29.29292098952351
22	31.548740452225065	20.072765235898512	20.387736251615117	27.990758060261307
23	31.720023106717615	19.043481282587233	19.16936506596101	30.067130544734145
24	32.098645955315554	20.24466441827045	19.881342670679206	27.775346955734793
25	34.95003134950853	18.514930063319284	19.331997647479074	27.203040939693114
26	35.34487074960075	18.06865729195547	19.024574427619253	27.561897530824524
27	33.69096932381464	19.531902141705757	19.417545561410286	27.359582973069312
28	32.620116445302024	18.35366131620108	19.71839248388994	29.307829754606956
29	30.313536187197293	20.471004880658885	18.904855923039385	30.310603009104437
30	30.874950210703073	19.565624348442128	20.136211559486718	29.423213881368078
31	31.325426581244546	18.096737334462265	19.906825823027354	30.671010261265835
32	31.748626263788736	19.151934141370962	18.696861835728576	30.402577759111722
33	30.575896823872554	20.643128254986966	20.464521996593778	28.316452924546702
34	31.39154452564345	20.067889060852423	19.428306775305106	29.112259638199024
35	33.92425973684348	21.530349238756212	16.849146463857643	27.696244560542667
36	35.097063907411716	19.52342021269838	18.280742882465873	27.098772997424035
37	34.717077224413906	19.3822166456549	19.554321337319962	26.346384792611232
38	33.10444580122095	21.766666243192972	20.656654503007534	24.472233452578543
39	32.20416563600643	21.950036580654178	22.225194841492552	23.62060294184685
40	33.62718671229222	22.765945839707236	18.58405591646309	25.02281153153746
41	33.66072209239847	22.835501394100312	18.07007717434437	25.433699339156846
42	34.58443031702985	21.78157500827642	18.134663140376297	25.499331534317438
43	33.712136781007054	21.429201301210114	17.791892322098075	27.066769595684754
44	31.95837652142266	21.949793706035024	18.678907796574197	27.412921975968118
45	30.368892917701373	20.672964467816872	19.762222011315718	29.195920603166037
46	29.894428007852696	19.657113349211105	20.382355644667708	30.06610299826849
47	28.87145879463942	17.96935893804754	23.901534145555882	29.257648121757157
48	29.194650182081233	18.14017452596479	22.6491231478942	30.016052144059774
49	31.03739596559102	17.89550637116174	21.87052316687579	29.196574496371454
50	30.71510134597377	19.534312205234283	22.10179585229935	27.64879059649259
51	31.14741816806774	18.864221130988636	23.332049211628984	26.65631148931464
52	31.440810708005674	18.255876257997112	22.552365636155887	27.75094739784133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	4.0
6	6.0
7	28.5
8	51.0
9	440.5
10	830.0
11	947.0
12	1064.0
13	2099.5
14	3025.5
15	2916.0
16	3312.0
17	3708.0
18	4448.5
19	5189.0
20	7395.5
21	9602.0
22	11380.5
23	13159.0
24	15326.0
25	17493.0
26	20815.5
27	24138.0
28	26584.5
29	29031.0
30	26297.5
31	23564.0
32	26322.0
33	29080.0
34	30459.0
35	31838.0
36	31057.0
37	30276.0
38	36894.0
39	49023.0
40	54534.0
41	74791.5
42	95049.0
43	102399.0
44	109749.0
45	131714.5
46	153680.0
47	160584.0
48	167488.0
49	219563.5
50	271639.0
51	324875.0
52	378111.0
53	439354.0
54	500597.0
55	543837.5
56	587078.0
57	614439.5
58	641801.0
59	604127.5
60	566454.0
61	516679.5
62	466905.0
63	409526.0
64	306399.5
65	260652.0
66	232215.0
67	203778.0
68	173833.5
69	143889.0
70	105267.5
71	66646.0
72	51338.0
73	36030.0
74	27955.5
75	19881.0
76	13324.0
77	6767.0
78	3700.0
79	633.0
80	385.0
81	137.0
82	215.0
83	293.0
84	157.5
85	22.0
86	12.0
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	5352556.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.335090301514732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44567956556043	5.03295554113029
2	8.027605049093433	1.0171120578180501
3	3.0654571852549517	0.5825984425205188
4	1.7950250717544196	0.4548658369218884
5	1.218085733798564	0.38583415593003695
6	0.9048809614212838	0.34395015616351815
7	0.6279108355241023	0.2784510291041328
8	0.48404938304690964	0.24531972415957343
9	0.3681132760203934	0.20988277602980496
>10	1.7065514905184542	1.9299950302980668
>50	0.1980397054992907	0.9043014494290217
>100	0.8994424356573237	17.41703025295294
>500	0.9180743872143382	41.45000376642861
>1k	0.34108491963609616	29.747699781113567
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.004894857709101969	0.0	0.0	0.0	0.0
12	0.007174142596546398	0.0	0.0	0.0	0.0
13	0.009901811396274976	0.0	0.0	0.0	0.0
14	0.019691526814478916	0.0	0.0	0.0	0.0
15	0.0344134652678085	0.0	0.0	0.0	0.0
16	0.04721108943091861	0.0	0.0	0.0	0.0
17	0.0566458342518976	0.0	0.0	0.0	0.0
18	0.06570692581263979	0.0	0.0	0.0	0.0
19	0.0768044276416725	0.0	0.0	0.0	0.0
20	0.08911630256647478	0.0	0.0	0.0	0.0
21	0.10273596390210583	0.0	0.0	0.0	0.0
22	0.11495442551184892	0.0	0.0	0.0	0.0
23	0.12737839641472223	0.0	0.0	0.0	0.0
24	0.1402881165559034	0.0	0.0	0.0	0.0
25	0.15439352712984228	0.0	0.0	0.0	0.0
26	0.16986277210364545	0.0	0.0	0.0	0.0
27	0.18555620903359069	0.0	0.0	0.0	0.0
28	0.1998297635746361	0.0	0.0	0.0	0.0
29	0.2187926665316533	0.0	0.0	0.0	0.0
30	0.24003485437611488	0.0	0.0	0.0	0.0
31	0.2571855390209836	0.0	0.0	0.0	0.0
32	0.27087993100866203	0.0	0.0	0.0	0.0
33	0.28349072854165375	0.0	0.0	0.0	0.0
34	0.2958960167815152	0.0	0.0	0.0	0.0
35	0.3067880093174177	0.0	0.0	0.0	0.0
36	0.3182965297327109	0.0	0.0	0.0	0.0
37	0.32739498661947675	0.0	0.0	0.0	0.0
38	0.3373341633417754	0.0	0.0	0.0	0.0
39	0.3481887905516542	0.0	0.0	0.0	0.0
40	0.3623502491146286	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGTA	20	6.3135324E-4	46.000004	28
AGCGTAA	20	6.3135324E-4	46.000004	40
AATTTCC	80	0.0	46.000004	33
TTAATAC	40	5.6188583E-9	46.000004	46
ACGTTAT	20	6.3135324E-4	46.000004	14
CCGATAT	20	6.3135324E-4	46.000004	25
CGATTTC	80	0.0	46.000004	40
TCGATAA	320	0.0	46.000004	36
GTCAGTA	20	6.3135324E-4	46.000004	43
AATGCGT	20	6.3135324E-4	46.000004	46
TCGGATA	20	6.3135324E-4	46.000004	43
ACCTAAC	20	6.3135324E-4	46.000004	24
TGACATA	40	5.6188583E-9	46.000004	41
TGATTGA	40	5.6188583E-9	46.000004	23
CGTAGAC	40	5.6188583E-9	46.000004	16
TCATGTA	40	5.6188583E-9	46.000004	30
ACTACGT	20	6.3135324E-4	46.000004	23
CTCATAT	40	5.6188583E-9	46.000004	46
AATTCGT	20	6.3135324E-4	46.000004	19
CTATTAG	80	0.0	46.000004	34
>>END_MODULE