FastQCFastQC Report
Fri 17 Jun 2016
SRR1528064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5317463
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAA351.0205076E-746.00000432
CGAACTA551.8189894E-1246.00000434
AATTTCC551.8189894E-1246.00000433
CTATATG351.0205076E-746.00000419
ATAATCG700.046.00000414
CGCATAT551.8189894E-1246.00000446
TACGGAC1100.046.00000435
GTAATGT551.8189894E-1246.00000430
GTAATAC351.0205076E-746.00000429
TAGTTAC351.0205076E-746.00000423
CGTTAAG351.0205076E-746.00000441
AAGTTAT351.0205076E-746.00000414
ATATAGC700.046.00000415
TCTAACG700.046.00000437
TCTAAAG1100.046.00000441
TCATGTA700.046.00000430
CCATAAT351.0205076E-746.00000443
ACGTGAC551.8189894E-1246.00000446
CTCATGT700.046.00000429
CTCATGC1100.046.00000436
Produced by FastQC (version 0.11.5)