FastQCFastQC Report
Fri 17 Jun 2016
SRR1528063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4773516
Sequences flagged as poor quality0
Sequence length59
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTAG150.006697552753.00000425
TACCTAA150.006697552753.00000420
CACGTTA11400.053.0000041
CGATCTA150.006697552753.00000425
CGTTTAC1550.053.00000425
CGATACA307.0151873E-753.00000423
GTACGTA600.053.00000427
TACCGAT307.0151873E-753.00000422
TAATCAA401.6025297E-953.053
TAATATC900.053.020
CTATGCG550.053.032
CGTATTG1000.053.017
AGTCATA203.1392492E-453.019
CCGATTA9650.053.01
ACGATAC700.053.041
TAACACC850.053.031
AATCTAT550.053.020
GTACTCA401.6025297E-953.030
CGAATAA457.6397555E-1153.041
ACTAATT401.6025297E-953.048
Produced by FastQC (version 0.11.5)