##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528063_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4773516
Sequences flagged as poor quality	0
Sequence length	59
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95727321328765	31.0	31.0	33.0	30.0	34.0
2	31.527591611717654	31.0	31.0	34.0	30.0	34.0
3	31.738886179495367	31.0	31.0	34.0	30.0	34.0
4	35.533042520439864	37.0	35.0	37.0	33.0	37.0
5	35.26139935427052	37.0	35.0	37.0	33.0	37.0
6	35.55229185363577	37.0	35.0	37.0	33.0	37.0
7	35.39454942646049	37.0	35.0	37.0	33.0	37.0
8	35.515850161599964	37.0	35.0	37.0	33.0	37.0
9	37.123768727286134	39.0	37.0	39.0	33.0	39.0
10	37.30067208321916	39.0	37.0	39.0	34.0	39.0
11	36.44196479073287	37.0	35.0	39.0	33.0	39.0
12	36.49467310887824	37.0	35.0	39.0	33.0	39.0
13	36.70490766135486	39.0	37.0	39.0	33.0	39.0
14	37.17254996107691	39.0	37.0	40.0	32.0	40.0
15	36.51611663184956	39.0	36.0	40.0	31.0	40.0
16	35.92036750269612	38.0	35.0	40.0	30.0	40.0
17	36.047536658513344	38.0	35.0	40.0	30.0	40.0
18	36.297996277796074	38.0	35.0	40.0	30.0	40.0
19	36.49522406544777	39.0	35.0	40.0	30.0	40.0
20	36.5131988664121	39.0	35.0	40.0	30.0	40.0
21	36.32857709076496	39.0	35.0	40.0	30.0	40.0
22	36.2837350917018	39.0	35.0	40.0	30.0	40.0
23	36.24179933617066	38.0	35.0	40.0	30.0	40.0
24	36.192607294078414	38.0	35.0	40.0	30.0	40.0
25	36.17494526047467	38.0	35.0	40.0	30.0	40.0
26	35.81013806175574	38.0	35.0	40.0	29.0	40.0
27	35.616684850328355	38.0	34.0	40.0	27.0	40.0
28	35.56081827315547	38.0	34.0	40.0	27.0	40.0
29	35.516454537912935	38.0	34.0	40.0	27.0	40.0
30	35.44264940140559	38.0	34.0	40.0	27.0	40.0
31	35.44431253608451	38.0	34.0	40.0	27.0	40.0
32	35.399504055291736	38.0	34.0	40.0	27.0	40.0
33	35.175445101681866	38.0	34.0	40.0	27.0	40.0
34	35.08058546362891	38.0	33.0	40.0	26.0	40.0
35	34.916776648491386	38.0	33.0	40.0	26.0	40.0
36	34.78885773086337	37.0	33.0	40.0	25.0	40.0
37	34.71944097390686	37.0	33.0	40.0	26.0	40.0
38	34.609532260916275	37.0	33.0	40.0	25.0	40.0
39	34.28851374961349	37.0	33.0	40.0	24.0	40.0
40	34.24362335854745	37.0	33.0	40.0	24.0	40.0
41	34.01821403761923	36.0	33.0	39.0	24.0	40.0
42	33.95344898812532	36.0	33.0	39.0	24.0	40.0
43	33.802759642996904	36.0	32.0	39.0	23.0	40.0
44	33.57661375807685	35.0	32.0	39.0	23.0	40.0
45	33.47464070509034	35.0	32.0	39.0	23.0	40.0
46	33.33030139628735	35.0	32.0	39.0	23.0	40.0
47	33.010772772103415	35.0	31.0	38.0	23.0	40.0
48	32.859246308172004	35.0	31.0	38.0	23.0	40.0
49	32.634083974998724	35.0	31.0	38.0	22.0	40.0
50	32.26252137837184	35.0	31.0	38.0	21.0	40.0
51	32.074830795581285	35.0	30.0	37.0	20.0	40.0
52	31.486641293335982	34.0	30.0	37.0	18.0	39.0
53	31.470883935447162	34.0	30.0	36.0	20.0	39.0
54	31.50568658406089	34.0	30.0	36.0	20.0	39.0
55	31.48044921185977	34.0	30.0	36.0	20.0	39.0
56	31.343051536854595	34.0	30.0	36.0	20.0	39.0
57	31.06831819564447	34.0	29.0	35.0	20.0	38.0
58	30.950049607040178	34.0	29.0	35.0	20.0	38.0
59	30.264161888218243	33.0	28.0	35.0	18.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	6.0
13	7.0
14	33.0
15	194.0
16	606.0
17	1612.0
18	3727.0
19	6898.0
20	11518.0
21	17897.0
22	26366.0
23	36556.0
24	49508.0
25	64466.0
26	81535.0
27	101318.0
28	122122.0
29	145512.0
30	171817.0
31	208697.0
32	254600.0
33	320123.0
34	432695.0
35	533597.0
36	656528.0
37	811276.0
38	668622.0
39	45679.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.610231954810665	22.691533871469165	29.173066561419297	19.52516761230087
2	24.852121580822185	21.44685384944766	34.65374369751772	19.047280872212433
3	19.311509587482266	22.42692807565744	36.08426157993395	22.17730075692634
4	19.16365211722345	21.59814694242148	37.19566457931638	22.042536361038696
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.41994538197841	0.4884659441803484	0.04596192827257728	0.045626745568675166
12	99.59036483799363	0.31808838600310546	0.04629711097647939	0.04524966502678529
13	99.15305615399635	0.5362085305674056	0.12718088721185808	0.18355442822439474
14	70.47310619677404	17.99019841978114	4.114891413373288	7.421803970071536
15	36.32033494807601	24.26527951304657	15.259171646224711	24.15521389265271
16	27.699582446146614	17.007421783021154	28.3026389772235	26.99035679360874
17	22.874669321313682	16.683593393213723	29.464675513814136	30.97706177165846
18	23.990639185036773	16.05523056799223	31.991324633666252	27.96280561330474
19	23.936297689166643	15.916716315604681	30.02828523042554	30.118700764803137
20	29.757478554591625	17.47514829739756	23.00995744017617	29.757415707834646
21	31.337278433758257	19.417259730563384	20.16452861999415	29.0809332156842
22	31.547375142347907	20.239567647830235	20.447799902629423	27.765257307192435
23	31.625284172086154	19.254046702682047	19.22388025933086	29.89678886590094
24	31.97626236090965	20.464957067285415	19.899399101207578	27.659381470597356
25	34.92402665037679	18.767864190672032	19.343687127056867	26.96442203189431
26	35.0441687008067	18.221432587635615	19.2449758207577	27.489422890799993
27	33.623873890859485	19.581101226014535	19.63525418161372	27.159770701512258
28	32.660893982548714	18.403248255583517	19.799640348958714	29.136217412909062
29	30.234129308459423	20.55652479220767	18.89049916246222	30.318846736870686
30	30.71647816829356	19.67928880933886	20.313580178635622	29.290652843731955
31	31.077847020938027	18.269573203483557	20.04229167766485	30.610288097913575
32	31.56639676079435	19.37115115985785	18.68559778578306	30.376854293564744
33	30.44196353379773	20.866652588993105	20.53346003239541	28.15792384481376
34	31.274641165966553	20.24520290703959	19.588119113877486	28.892036813116366
35	33.76050274053758	21.648822377467678	17.060925322131524	27.529749559863216
36	34.96764229972205	19.604689708801644	18.53721659254939	26.890451398926913
37	34.64733332830559	19.485909338106335	19.58409692143066	26.282660412157412
38	33.04231095067032	21.868492742037525	20.66767975638921	24.42151655090294
39	31.962666512482624	22.16014359227035	22.367600737066766	23.509589158180262
40	33.36953306535476	23.14503606984872	18.566000407246985	24.91943045754953
41	33.503103372859755	23.05824469845707	18.18822855103031	25.250423377652865
42	34.5007746910244	22.01735576040805	18.09211491068638	25.389754637881172
43	33.48569482117584	21.69815708169827	17.840287955460923	26.97586014166497
44	31.842461615295726	22.118287652120575	18.637645710206062	27.401605022377634
45	30.137743332168576	20.930923872466334	19.679582094204775	29.25175070116032
46	29.778385575747517	19.919656705874665	20.320074343523725	29.98188337485409
47	28.712399832743827	18.170568612318466	23.823843892007485	29.29318766293022
48	29.066939337796292	18.240391359325077	22.53793639740602	30.154732905472613
49	30.862680673951864	18.08694052769489	21.884581511824827	29.165797286528424
50	30.47468574526617	19.630833959706013	22.261724900471684	27.63275539455613
51	30.835028100879942	19.00404230340906	23.526599680403294	26.63432991530771
52	31.034398962944714	18.586509398942	22.813330886499593	27.565760751613695
53	28.600721145587443	21.32920472037802	23.19870720031105	26.871366933723483
54	28.771203448359657	20.670403115858416	22.995712175260334	27.562681260521593
55	28.711876109768987	20.823518764784698	21.867110113383927	28.597495012062385
56	30.242802160922892	19.67583223770487	21.67402392701732	28.407341674354917
57	29.278502470715505	20.022453051377642	22.065244989228066	28.633799488678786
58	30.233752227917538	19.49954708437135	21.828543153516193	28.438157534194918
59	28.321702493507928	20.166162635675676	23.36145935197452	28.15067551884188
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	1.0
5	2.0
6	17.0
7	32.0
8	292.5
9	633.5
10	714.0
11	1402.0
12	2090.0
13	1865.5
14	1612.5
15	1584.0
16	1662.5
17	1741.0
18	2161.5
19	3200.0
20	3818.0
21	5084.5
22	6351.0
23	8198.5
24	10046.0
25	12128.5
26	15905.5
27	17600.0
28	19878.5
29	22157.0
30	23058.5
31	25804.5
32	27649.0
33	27127.5
34	26606.0
35	28565.0
36	31903.0
37	33282.0
38	38772.5
39	44263.0
40	48022.5
41	51782.0
42	58903.5
43	77232.0
44	88439.0
45	93745.5
46	99052.0
47	113852.5
48	153910.5
49	179168.0
50	201645.0
51	224122.0
52	276634.5
53	367856.0
54	406565.0
55	437816.0
56	469067.0
57	480082.0
58	467478.0
59	443859.0
60	424344.5
61	404830.0
62	362346.5
63	319863.0
64	277255.0
65	211730.0
66	188813.0
67	175153.0
68	161493.0
69	138098.0
70	83977.0
71	53251.0
72	39047.0
73	24843.0
74	19529.0
75	9555.5
76	4896.0
77	3636.5
78	2377.0
79	1669.0
80	961.0
81	560.5
82	103.0
83	46.0
84	38.0
85	30.0
86	16.0
87	2.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	4773516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.515343675251986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56571916996322	10.083076638559268
2	8.682977883715226	2.1734090467861646
3	3.4949814524155216	1.3122268204683478
4	1.8909053670361387	0.9466132210334309
5	1.1395676722498638	0.7131040529706981
6	0.7408426141792614	0.5563139955435339
7	0.5127373804428929	0.44919591719738605
8	0.34317720820419956	0.343598456175125
9	0.26747829566526804	0.3012824516329343
>10	1.0266957397798826	2.291118512058008
>50	0.12421618810309609	1.1255656974142894
>100	0.6684005966300536	25.4832313579404
>500	0.4380320394497558	37.25289552525825
>1k	0.10426839216567153	16.968368306962155
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.005006791639537817	0.0	0.0	0.0	0.0
12	0.0071645302959076704	0.0	0.0	0.0	0.0
13	0.009112779762338704	0.0	0.0	0.0	0.0
14	0.01923110763638375	0.0	0.0	0.0	0.0
15	0.033643963904174616	0.0	0.0	0.0	0.0
16	0.04629711097647939	0.0	0.0	0.0	0.0
17	0.05436244478912399	0.0	0.0	0.0	0.0
18	0.06282580806265235	0.0	0.0	0.0	0.0
19	0.07332121647858728	0.0	0.0	0.0	0.0
20	0.08549253841403276	0.0	0.0	0.0	0.0
21	0.09734962656457001	0.0	0.0	0.0	0.0
22	0.10845255363132751	0.0	0.0	0.0	0.0
23	0.11930409367015843	0.0	0.0	0.0	0.0
24	0.1314754156056039	0.0	0.0	0.0	0.0
25	0.14463133673376186	0.0	0.0	0.0	0.0
26	0.15837382759374852	0.0	0.0	0.0	0.0
27	0.17303807088946596	0.0	0.0	0.0	0.0
28	0.1873671314812813	0.0	0.0	0.0	0.0
29	0.20454524505626462	0.0	0.0	0.0	0.0
30	0.2240696375585627	0.0	0.0	0.0	0.0
31	0.24049359004976625	0.0	0.0	0.0	0.0
32	0.25172221063048705	0.0	0.0	0.0	0.0
33	0.2635583498620304	0.0	0.0	0.0	0.0
34	0.27392806476400205	0.0	0.0	0.0	0.0
35	0.2841720861520104	0.0	0.0	0.0	0.0
36	0.29529596213776177	0.0	0.0	0.0	0.0
37	0.30373837649229624	0.0	0.0	0.0	0.0
38	0.31308159436356764	0.0	0.0	0.0	0.0
39	0.32296948412868	0.0	0.0	0.0	0.0
40	0.3356226312009848	0.0	0.0	0.0	0.0
41	0.3453219806951522	0.0	0.0	0.0	0.0
42	0.355126074784289	0.0	0.0	0.0	0.0
43	0.3634008977868724	0.0	0.0	0.0	0.0
44	0.37209469916933346	0.0	0.0	0.0	0.0
45	0.38106083649871497	0.0	0.0	0.0	0.0
46	0.38967084220520054	0.0	0.0	0.0	0.0
47	0.39790376736979616	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTAG	15	0.0066975527	53.000004	25
TACCTAA	15	0.0066975527	53.000004	20
CACGTTA	1140	0.0	53.000004	1
CGATCTA	15	0.0066975527	53.000004	25
CGTTTAC	155	0.0	53.000004	25
CGATACA	30	7.0151873E-7	53.000004	23
GTACGTA	60	0.0	53.000004	27
TACCGAT	30	7.0151873E-7	53.000004	22
TAATCAA	40	1.6025297E-9	53.0	53
TAATATC	90	0.0	53.0	20
CTATGCG	55	0.0	53.0	32
CGTATTG	100	0.0	53.0	17
AGTCATA	20	3.1392492E-4	53.0	19
CCGATTA	965	0.0	53.0	1
ACGATAC	70	0.0	53.0	41
TAACACC	85	0.0	53.0	31
AATCTAT	55	0.0	53.0	20
GTACTCA	40	1.6025297E-9	53.0	30
CGAATAA	45	7.6397555E-11	53.0	41
ACTAATT	40	1.6025297E-9	53.0	48
>>END_MODULE