FastQCFastQC Report
Fri 17 Jun 2016
SRR1528059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521992
Sequences flagged as poor quality0
Sequence length52
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCACGTTGGGGGATGCTGAAACTGAAACTCCAGTACTTTGGCCACCTGAT40230.2643246482241694No Hit
GACCACGTTGGGGAGGTACTGGAGGGAGAGACGTGGGCACAAAGGTCGCGGG22900.150460712014255No Hit
TACCACGTTGGGGGGAATTGTGAAGTGATGAGCTAAACTGGGTGAGTTGGAG18720.12299670431907658No Hit
AATCACGTTGGGGCGGGAGTATGATGAGGCGTGCCATCCTGCAGGAAGCTGC16980.11156431834070087No Hit
GCTTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15850.10413983779152583No Hit
GGCGACGTTGGGGTTAAGTACAACTACGACTGCCCCCTTGAGACAAGGCCTG15630.10269436370230593No Hit
CTTTACGTTGGGGGATGGCAAAAATTCACCACCTCTTTGACCCGTGAAATAT15560.10223444012846322No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGGT1050.046.00000445
AGGTACT2300.046.00000414
GACAAGG1650.046.00000442
CCGTGAA1650.046.00000442
CATTATA1050.046.00000420
TATGAAG1050.046.00000425
CTAAACT2100.046.00000433
CAAGGCC1650.046.00000444
CTTTGGC4250.046.00000438
ATGCGCT1050.046.00000414
CTACAAT1050.046.00000439
ACCCGTG1650.046.00000440
CAGTACT4250.046.00000433
CAATAGA1050.046.00000442
CTGGCTG2100.046.00000417
CTAATTG1150.046.00000436
CTGTGTG1050.046.00000419
TACAATA1450.046.00000440
GTGTTGT453.110472E-1046.017
CTTAAGT1300.046.039
Produced by FastQC (version 0.11.5)