##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1528059_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1521992 Sequences flagged as poor quality 0 Sequence length 52 %GC 37 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.865983526851654 31.0 31.0 33.0 28.0 34.0 2 31.58118242408633 31.0 31.0 34.0 30.0 34.0 3 31.82361208206088 31.0 31.0 34.0 30.0 34.0 4 35.52644691956331 37.0 35.0 37.0 33.0 37.0 5 35.363445405757716 37.0 35.0 37.0 33.0 37.0 6 35.66888656444975 37.0 35.0 37.0 33.0 37.0 7 35.55954893324012 37.0 35.0 37.0 33.0 37.0 8 34.28302382666926 35.0 35.0 37.0 31.0 37.0 9 37.09799263071028 39.0 37.0 39.0 34.0 39.0 10 36.84141112436859 39.0 37.0 39.0 33.0 39.0 11 37.54401665711778 39.0 37.0 39.0 35.0 39.0 12 37.567934654058625 39.0 37.0 39.0 35.0 39.0 13 37.35681725002497 39.0 37.0 39.0 35.0 39.0 14 37.308808456286236 40.0 37.0 40.0 31.0 40.0 15 35.88127992788398 38.0 35.0 40.0 30.0 40.0 16 36.24410246571598 38.0 35.0 40.0 30.0 40.0 17 36.00419975926286 38.0 35.0 40.0 29.0 40.0 18 36.398883174155976 38.0 35.0 40.0 30.0 40.0 19 36.61163002170839 39.0 35.0 40.0 31.0 40.0 20 36.75740411250519 39.0 35.0 40.0 31.0 40.0 21 36.78594171322845 39.0 35.0 40.0 31.0 40.0 22 36.84776529705807 39.0 35.0 40.0 31.0 40.0 23 36.7817531235381 39.0 35.0 40.0 31.0 40.0 24 36.66294895111144 39.0 35.0 40.0 31.0 40.0 25 36.56436958932767 39.0 35.0 40.0 31.0 40.0 26 36.15645351618142 39.0 35.0 40.0 30.0 40.0 27 35.41282542878018 37.0 35.0 40.0 29.0 40.0 28 34.830790832014884 35.0 34.0 40.0 28.0 40.0 29 34.69618959889408 35.0 33.0 40.0 28.0 40.0 30 34.50148489610984 35.0 33.0 40.0 27.0 40.0 31 34.25790608623436 35.0 33.0 40.0 26.0 40.0 32 33.950286203869666 35.0 33.0 39.0 23.0 40.0 33 33.70159238681938 35.0 33.0 39.0 22.0 40.0 34 33.45160421342556 35.0 33.0 39.0 21.0 40.0 35 33.23406759036841 35.0 33.0 39.0 21.0 40.0 36 32.80942081167312 35.0 33.0 39.0 20.0 40.0 37 32.391700481999905 35.0 32.0 39.0 17.0 40.0 38 31.710411092830974 35.0 31.0 39.0 15.0 40.0 39 30.807859699656767 35.0 28.0 38.0 10.0 40.0 40 29.93503185299266 35.0 25.0 37.0 9.0 40.0 41 28.92229722626663 35.0 21.0 37.0 8.0 40.0 42 27.92666518615078 34.0 18.0 35.0 8.0 40.0 43 27.013547377384374 33.0 15.0 35.0 8.0 40.0 44 26.296413515971174 33.0 11.0 35.0 8.0 39.0 45 25.876557169814298 33.0 10.0 35.0 8.0 39.0 46 25.41914609275213 33.0 10.0 35.0 8.0 39.0 47 24.881760876535488 32.0 10.0 35.0 8.0 38.0 48 24.439686279559947 31.0 9.0 35.0 8.0 38.0 49 24.116029519209036 31.0 9.0 35.0 8.0 37.0 50 23.72210563524644 30.0 9.0 35.0 8.0 37.0 51 23.307393205746155 28.0 9.0 35.0 8.0 36.0 52 22.517736624108405 26.0 8.0 35.0 7.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 2.0 13 2.0 14 17.0 15 69.0 16 225.0 17 622.0 18 1604.0 19 3327.0 20 6346.0 21 10206.0 22 15435.0 23 22370.0 24 30400.0 25 41369.0 26 53893.0 27 67956.0 28 85723.0 29 102443.0 30 109183.0 31 94495.0 32 76255.0 33 76630.0 34 96087.0 35 126541.0 36 161691.0 37 198465.0 38 123796.0 39 16838.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.76070045046229 20.634011216878932 39.782666400349015 3.8226219323097625 2 22.4836267207712 22.250379765465258 35.915169067905744 19.350824445857796 3 19.41429389904809 22.9652324059522 37.733246955305944 19.88722673969377 4 15.557703325641658 20.122247685927388 36.52483061671809 27.79521837171286 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 0.0 0.0 100.0 0.0 9 0.0 0.0 100.0 0.0 10 100.0 0.0 0.0 0.0 11 99.43061461558274 0.31025130224074765 0.1791073803278861 0.08002670184862995 12 98.72246371860037 0.7298330083206744 0.20190644891694567 0.3457968241620193 13 92.70686048284091 4.818093656208442 1.290085624628776 1.1849602363218728 14 39.25138896919301 39.33279544176316 11.25321289468013 10.162602694363702 15 24.54415003495419 56.575593038596786 12.942643588139754 5.937613338309268 16 31.630849570825603 49.015894958712 12.35518977760724 6.998065692855153 17 16.387076936015433 52.12484691115328 18.28018806931968 13.207888083511609 18 16.061713859205568 55.025387781276116 17.68156468627956 11.231333673238755 19 20.494325857166135 52.62866033461411 18.96527708424223 7.911736723977524 20 15.751002633390975 55.459752745086696 19.41468811925424 9.37455650226808 21 18.807391891678797 52.931290046202605 14.080165992988135 14.181152069130457 22 19.681640902186082 59.37166555408964 12.197304585043812 8.749388958680466 23 14.324516817433993 55.76409074423518 14.274450851252832 15.636941587077988 24 18.77329184384675 58.401095406546155 10.77554941156064 12.050063338046455 25 18.996946107469682 51.04731168100752 17.92992341615462 12.025818795368174 26 18.938995737165502 52.4561232910554 18.029464018207715 10.575416953571372 27 21.63204537211759 54.991944767120984 14.69081309231586 8.685196768445563 28 23.580150224179892 54.989053818942544 13.652305662579042 7.778490294298525 29 26.420835326335485 53.769862128053234 10.755115664208486 9.054186881402792 30 27.46532176253226 55.740963158807666 9.252348238361305 7.541366840298766 31 20.184337368396154 60.41214408485721 11.476078717890765 7.927439828855867 32 17.837872998018387 64.18765670253195 10.6434199391324 7.331050360317268 33 16.129651141398902 66.75238766038191 8.85779951537196 8.260161682847215 34 13.610584024094738 68.73143879862707 9.138681412254467 8.519295765023733 35 15.874919184857738 67.00803946407076 9.314306514094687 7.802734836976804 36 12.351378982281116 66.4828724461101 12.655059947752681 8.510688623856105 37 13.643632818043722 66.89903757707005 11.198876209598998 8.258453395287228 38 15.091537931868235 64.60217924929961 12.020102602378987 8.286180216453175 39 15.049619183280857 62.88942386030938 12.504270718899967 9.55668623750979 40 18.025193299307748 61.86215170644787 11.10912540933198 9.003529584912403 41 19.036171017981697 60.190132405426574 10.532841171307076 10.240855405284654 42 20.982764692587082 56.67119143858837 10.316085761291781 12.02995810753276 43 22.395847021534937 55.49937187580487 11.686263791136879 10.418517311523319 44 22.01897250445469 53.04778211712019 14.001584765228728 10.931660613196389 45 22.49532192022034 52.498830480055084 13.40204153504092 11.603806064683651 46 24.07548791320848 53.07610026859537 11.864779841155539 10.983631977040616 47 23.958273105246285 52.71998801570573 12.405321447156096 10.91641743189189 48 23.587509001361372 52.748371870548596 11.558602147711683 12.105516980378345 49 22.424493689848568 53.175180947074615 11.4212164058681 12.97910895720871 50 22.224624045330067 53.401134828566775 12.264453426824845 12.109787699278314 51 21.850246256222107 53.6875358083354 11.959326987264058 12.502890948178441 52 24.149995532170998 50.136728708166665 12.701249415240026 13.012026344422308 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 2.0 6 4.0 7 15.5 8 27.0 9 432.5 10 838.0 11 2806.0 12 4774.0 13 11354.0 14 22567.5 15 27201.0 16 27261.5 17 27322.0 18 24218.5 19 21115.0 20 23213.5 21 25312.0 22 31555.5 23 37799.0 24 45797.5 25 53796.0 26 64578.5 27 75361.0 28 87884.0 29 100407.0 30 108790.5 31 117174.0 32 116887.0 33 116600.0 34 113044.0 35 109488.0 36 101269.5 37 93051.0 38 85116.5 39 71474.5 40 65767.0 41 64539.0 42 63311.0 43 61408.0 44 59505.0 45 60086.0 46 60667.0 47 63312.5 48 65958.0 49 68347.5 50 70737.0 51 66723.0 52 62709.0 53 60894.5 54 59080.0 55 51908.0 56 44736.0 57 36745.5 58 28755.0 59 23681.0 60 18607.0 61 13256.0 62 7905.0 63 5734.5 64 3977.5 65 4391.0 66 2419.0 67 447.0 68 321.0 69 195.0 70 152.5 71 110.0 72 76.5 73 43.0 74 46.5 75 50.0 76 33.0 77 16.0 78 14.0 79 12.0 80 7.5 81 3.0 82 3.5 83 4.0 84 6.0 85 8.0 86 13.0 87 18.0 88 13.5 89 4.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1521992.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.03563408627283 #Duplication Level Percentage of deduplicated Percentage of total 1 89.50873561621131 47.4715254966632 2 4.8438630142927614 5.13794692780123 3 1.4530314449920498 2.311873320973399 4 0.6992085688542187 1.483318792309554 5 0.44440924023461315 1.178476292482073 6 0.30169841643341483 0.960046009102233 7 0.22537364441468707 0.836698389650698 8 0.19569838294767658 0.8303190263430617 9 0.16145470320361816 0.7706567304553387 >10 1.9676730936015112 22.374375604170204 >50 0.13224371992910608 4.650496110962446 >100 0.05374232387762043 6.234292203724944 >500 0.00996626127047165 3.4893449938238534 >1k 0.002901569736972759 2.2706301015378147 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTCACGTTGGGGGATGCTGAAACTGAAACTCCAGTACTTTGGCCACCTGAT 4023 0.2643246482241694 No Hit GACCACGTTGGGGAGGTACTGGAGGGAGAGACGTGGGCACAAAGGTCGCGGG 2290 0.150460712014255 No Hit TACCACGTTGGGGGGAATTGTGAAGTGATGAGCTAAACTGGGTGAGTTGGAG 1872 0.12299670431907658 No Hit AATCACGTTGGGGCGGGAGTATGATGAGGCGTGCCATCCTGCAGGAAGCTGC 1698 0.11156431834070087 No Hit GCTTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1585 0.10413983779152583 No Hit GGCGACGTTGGGGTTAAGTACAACTACGACTGCCCCCTTGAGACAAGGCCTG 1563 0.10269436370230593 No Hit CTTTACGTTGGGGGATGGCAAAAATTCACCACCTCTTTGACCCGTGAAATAT 1556 0.10223444012846322 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.009855505153772162 0.0 0.0 0.0 0.0 12 0.05591356590573406 0.0 0.0 0.0 0.0 13 0.2500013140673538 0.0 0.0 0.0 0.0 14 0.6754306198718522 0.0 0.0 0.0 0.0 15 1.230558373499992 0.0 0.0 0.0 0.0 16 1.5697191575251381 0.0 0.0 0.0 0.0 17 1.7668292606005813 0.0 0.0 0.0 0.0 18 2.039169719683152 0.0 0.0 0.0 0.0 19 2.2730080052983195 0.0 0.0 0.0 0.0 20 2.504875189882733 0.0 0.0 0.0 0.0 21 2.7090155533012 0.0 0.0 0.0 0.0 22 2.9541548181593598 0.0 0.0 0.0 0.0 23 3.203236285079028 0.0 0.0 0.0 0.0 24 3.4947621275276086 0.0 0.0 0.0 0.0 25 3.8087585217267894 0.0 0.0 0.0 0.0 26 4.138260910701239 0.0 0.0 0.0 0.0 27 4.469865807441826 0.0 0.0 0.0 0.0 28 4.80278477153625 0.0 0.0 0.0 0.0 29 5.153903568481306 0.0 0.0 0.0 0.0 30 5.537151312227659 0.0 0.0 0.0 0.0 31 5.879334451166629 0.0 0.0 0.0 0.0 32 6.167903642069078 0.0 0.0 0.0 0.0 33 6.446551624450063 0.0 0.0 0.0 0.0 34 6.701152174255844 0.0 0.0 0.0 0.0 35 6.945108778495551 0.0 0.0 0.0 0.0 36 7.174807751946134 0.0 0.0 0.0 0.0 37 7.394716923610637 0.0 0.0 0.0 0.0 38 7.609041309021335 0.0 0.0 0.0 0.0 39 7.8254024988304804 0.0 0.0 0.0 0.0 40 8.051093566851863 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAGGT 105 0.0 46.000004 45 AGGTACT 230 0.0 46.000004 14 GACAAGG 165 0.0 46.000004 42 CCGTGAA 165 0.0 46.000004 42 CATTATA 105 0.0 46.000004 20 TATGAAG 105 0.0 46.000004 25 CTAAACT 210 0.0 46.000004 33 CAAGGCC 165 0.0 46.000004 44 CTTTGGC 425 0.0 46.000004 38 ATGCGCT 105 0.0 46.000004 14 CTACAAT 105 0.0 46.000004 39 ACCCGTG 165 0.0 46.000004 40 CAGTACT 425 0.0 46.000004 33 CAATAGA 105 0.0 46.000004 42 CTGGCTG 210 0.0 46.000004 17 CTAATTG 115 0.0 46.000004 36 CTGTGTG 105 0.0 46.000004 19 TACAATA 145 0.0 46.000004 40 GTGTTGT 45 3.110472E-10 46.0 17 CTTAAGT 130 0.0 46.0 39 >>END_MODULE