FastQCFastQC Report
Fri 17 Jun 2016
SRR1528058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509919
Sequences flagged as poor quality0
Sequence length52
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCACGTTGGGGGATGCTGAAACTGAAACTCCAGTACTTTGGCCACCTGAT39090.25888805955816174No Hit
GACCACGTTGGGGAGGTACTGGAGGGAGAGACGTGGGCACAAAGGTCGCGGG21960.1454382652314462No Hit
TACCACGTTGGGGGGAATTGTGAAGTGATGAGCTAAACTGGGTGAGTTGGAG17720.11735728870224163No Hit
AATCACGTTGGGGCGGGAGTATGATGAGGCGTGCCATCCTGCAGGAAGCTGC16730.11080064559754531No Hit
CTTTACGTTGGGGGATGGCAAAAATTCACCACCTCTTTGACCCGTGAAATAT15330.10152862504544946No Hit
GGCGACGTTGGGGTTAAGTACAACTACGACTGCCCCCTTGAGACAAGGCCTG15290.10126371017253244No Hit
GCTTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15280.10119748145430318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCT1550.046.00000419
TATTTAG351.0198528E-746.00000414
CGTGGTT700.046.00000442
TCCGCCG700.046.00000420
TGCCAAT351.0198528E-746.00000435
CATTGTT700.046.00000446
ACCGCCA351.0198528E-746.00000435
GACCGTC700.046.00000428
ACACAAT700.046.00000436
CTAAGTT1550.046.00000424
TGTGCTT351.0198528E-746.00000414
CCTAAGT1550.046.00000423
CCAGACC700.046.00000425
TTTCACA1400.046.00000438
ATACTCA351.0198528E-746.00000417
ATTTTTC1400.046.00000435
CTTATAA1400.046.00000415
TCTAACT351.0198528E-746.00000428
GATATTT1400.046.00000432
ACAGCTT700.046.00000439
Produced by FastQC (version 0.11.5)