##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528058_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1509919
Sequences flagged as poor quality	0
Sequence length	52
%GC	37
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.905568444399997	31.0	31.0	33.0	28.0	34.0
2	31.611475847379893	31.0	31.0	34.0	30.0	34.0
3	31.84611624861996	31.0	31.0	34.0	30.0	34.0
4	35.56458260343767	37.0	35.0	37.0	33.0	37.0
5	35.49572460509471	37.0	35.0	37.0	33.0	37.0
6	35.71568872237518	37.0	35.0	37.0	33.0	37.0
7	35.55023878764358	37.0	35.0	37.0	33.0	37.0
8	34.30153339351316	35.0	35.0	37.0	31.0	37.0
9	37.11416970049387	39.0	37.0	39.0	34.0	39.0
10	36.799759457295394	39.0	37.0	39.0	33.0	39.0
11	37.53123313237332	39.0	37.0	39.0	35.0	39.0
12	37.59053631353735	39.0	37.0	39.0	35.0	39.0
13	37.35354214365142	39.0	37.0	39.0	35.0	39.0
14	37.268038881555896	40.0	37.0	40.0	31.0	40.0
15	35.89512483782243	38.0	35.0	40.0	30.0	40.0
16	36.22321329819679	38.0	35.0	40.0	30.0	40.0
17	36.02443309872914	38.0	35.0	40.0	29.0	40.0
18	36.33603921799779	38.0	35.0	40.0	30.0	40.0
19	36.634211504060815	39.0	35.0	40.0	31.0	40.0
20	36.77975441066706	39.0	35.0	40.0	31.0	40.0
21	36.863356246262214	39.0	35.0	40.0	31.0	40.0
22	36.74565920423546	39.0	35.0	40.0	31.0	40.0
23	36.71784512944072	39.0	35.0	40.0	31.0	40.0
24	36.76572186984865	39.0	35.0	40.0	31.0	40.0
25	36.610642027817384	39.0	35.0	40.0	31.0	40.0
26	36.08112951754366	39.0	35.0	40.0	30.0	40.0
27	35.30549784458636	37.0	35.0	40.0	28.0	40.0
28	34.778258303922264	35.0	34.0	40.0	28.0	40.0
29	34.64906329412373	35.0	33.0	40.0	27.0	40.0
30	34.323009379973364	35.0	33.0	40.0	26.0	40.0
31	34.15331087296736	35.0	33.0	40.0	26.0	40.0
32	33.93254803734505	35.0	33.0	39.0	23.0	40.0
33	33.787344884063316	35.0	33.0	39.0	23.0	40.0
34	33.59157345526482	35.0	33.0	39.0	22.0	40.0
35	33.184107226943965	35.0	33.0	39.0	21.0	40.0
36	32.80919903650461	35.0	33.0	39.0	20.0	40.0
37	32.38924405878726	35.0	32.0	39.0	17.0	40.0
38	31.763442277367197	35.0	31.0	39.0	15.0	40.0
39	30.871874584000864	35.0	29.0	38.0	10.0	40.0
40	29.925280760093752	35.0	25.0	37.0	9.0	40.0
41	28.887397270979438	35.0	21.0	37.0	8.0	40.0
42	27.89099150351774	34.0	18.0	35.0	8.0	40.0
43	27.024274149805386	33.0	15.0	35.0	8.0	40.0
44	26.23800482012611	33.0	10.0	35.0	8.0	39.0
45	25.821094376585762	33.0	10.0	35.0	8.0	39.0
46	25.352256644230586	33.0	10.0	35.0	8.0	39.0
47	24.912364835464682	32.0	10.0	35.0	8.0	38.0
48	24.468559571738616	31.0	9.0	35.0	8.0	38.0
49	24.00483535871792	30.0	9.0	35.0	8.0	37.0
50	23.602780678963573	29.0	9.0	35.0	8.0	37.0
51	23.201690289346647	27.0	9.0	35.0	8.0	36.0
52	22.31146107837573	24.0	8.0	35.0	7.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	6.0
14	9.0
15	52.0
16	191.0
17	610.0
18	1549.0
19	3380.0
20	6063.0
21	10238.0
22	15369.0
23	22081.0
24	30716.0
25	41221.0
26	53747.0
27	68157.0
28	85114.0
29	101968.0
30	108527.0
31	93260.0
32	75533.0
33	76402.0
34	95942.0
35	124948.0
36	159361.0
37	196997.0
38	123201.0
39	15273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.84675734261242	20.663691231119017	39.64431204587795	3.845239380390604
2	22.485510812169395	22.193111021187228	35.97815511957926	19.343223047064114
3	19.354084556853714	22.932488431498644	37.76083352815615	19.952593483491498
4	15.528978706804802	20.126245182688606	36.49156014329245	27.853215967214133
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.4256645555159	0.3147188690254245	0.17934736896482525	0.08026920649385827
12	98.75609221421811	0.7066604235061615	0.20279233521798187	0.3344550270577428
13	92.76543973550899	4.777673504340299	1.2854331921116298	1.1714535680390803
14	39.38900033710417	39.309790790101985	11.207952214655222	10.093256658138614
15	24.487075134493967	56.64793939277537	12.829959752807932	6.035025719922724
16	31.76971744841942	48.9450758616853	12.329204414276527	6.956002275618759
17	16.335048436373075	52.08127058471348	18.34727558233256	13.236405396580878
18	16.033906454584653	55.05421151730656	17.67101414049363	11.240867887615163
19	20.65746573160547	52.60043750691262	18.90399418776769	7.8381025737142185
20	15.70435235267587	55.448139933334176	19.47627654198669	9.371231172003267
21	18.782398261098773	52.9678082069303	14.09863707920756	14.151156452763361
22	19.73536328769954	59.384642487444694	12.168202400261206	8.711791824594565
23	14.261294811178612	55.76636892442575	14.273745810205712	15.698590454189926
24	18.876575498420774	58.428233567496	10.673022857517523	12.022168076565697
25	18.83008293822384	51.136981520200756	18.015867076313366	12.017068465262044
26	18.848958122919175	52.43532931236709	18.047789318499866	10.66792324621387
27	21.66142687124276	54.82002676964791	14.698205665337014	8.820340693772314
28	23.601001113304754	54.978180948779375	13.640003205469961	7.780814732445912
29	26.320484741234463	53.74738644920688	10.831243265367215	9.100885544191444
30	27.44650540856827	55.69523928104753	9.35566742321939	7.5025878871648075
31	20.11452269956203	60.454633659156556	11.488695751229038	7.94214789005238
32	17.801617172841723	64.30252218827633	10.606131852105975	7.28972878677598
33	16.132918388337387	66.83133333642401	8.744839954990963	8.290908320247642
34	13.599537458631886	68.85051449779756	9.052538579884088	8.497409463686463
35	15.77660788426399	66.95206828975594	9.471633908838818	7.799689917141251
36	12.348278285126552	66.52754220590641	12.618623912938375	8.50555559602866
37	13.615432351006909	66.91286088856423	11.191130120224992	8.280576640203877
38	15.10458508039173	64.66949551598464	11.954813470126544	8.271105933497095
39	15.004712173302012	62.99821381146936	12.470072897950155	9.527001117278475
40	18.087923921746796	61.86133163434595	11.091985729035796	8.958758714871461
41	18.989627920438114	60.26608049835785	10.489238164431338	10.255053416772688
42	20.929467077373022	56.71595628639682	10.338302915586862	12.016273720643293
43	22.4789541690647	55.48158543603995	11.662479907862606	10.376980487032748
44	21.984358101328613	52.96827180795791	14.140294942973764	10.907075147739713
45	22.520413346676214	52.485067079757265	13.424097584042588	11.570421989523942
46	23.994929529332367	53.172123802667556	11.81983934237532	11.013107325624752
47	23.951351032737517	52.842702158195245	12.302514240830137	10.903432568237104
48	23.70557625938875	52.81707164424052	11.403062018558611	12.07429007781212
49	22.226159151583627	53.21914619261033	11.685262586933472	12.86943206887257
50	22.303911666784774	53.46942451879869	12.182838946989872	12.043824867426663
51	21.825607863733087	53.68029675764065	11.971436878402086	12.522658500224185
52	24.356737017018794	49.95671953263718	12.763598577142218	12.922944873201809
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	2.5
6	5.0
7	12.0
8	19.0
9	409.0
10	799.0
11	2775.5
12	4752.0
13	11058.0
14	22068.0
15	26772.0
16	26910.0
17	27048.0
18	24071.0
19	21094.0
20	22849.5
21	24605.0
22	30855.5
23	37106.0
24	45291.5
25	53477.0
26	64232.5
27	74988.0
28	87609.5
29	100231.0
30	108375.5
31	116520.0
32	116303.5
33	116087.0
34	112343.5
35	108600.0
36	100718.0
37	92836.0
38	84684.0
39	70974.0
40	65416.0
41	64128.0
42	62840.0
43	60867.5
44	58895.0
45	59641.0
46	60387.0
47	62857.0
48	65327.0
49	68246.5
50	71166.0
51	66894.5
52	62623.0
53	60125.5
54	57628.0
55	50627.0
56	43626.0
57	36001.5
58	28377.0
59	23413.0
60	18449.0
61	13079.5
62	7710.0
63	5593.5
64	3858.0
65	4239.0
66	2358.0
67	477.0
68	326.5
69	176.0
70	144.5
71	113.0
72	85.0
73	57.0
74	47.5
75	38.0
76	27.0
77	16.0
78	13.5
79	11.0
80	7.5
81	4.0
82	2.0
83	0.0
84	3.0
85	6.0
86	14.0
87	22.0
88	13.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1509919.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.225145522265734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.5378560475342	47.65665417881689
2	4.822886635008474	5.1339768597143305
3	1.468170678490126	2.3443079404248177
4	0.6969049248792356	1.4837146416752396
5	0.4287099567551784	1.140907491756931
6	0.30193113081616557	0.96421970252356
7	0.2266290369588219	0.8443654430192965
8	0.19756675510930807	0.8412415432843807
9	0.16026313326827965	0.7677025731052606
>10	1.9657195605000641	22.461816498919475
>50	0.1270135236410993	4.461326646539985
>100	0.054193180014854556	6.392527392088342
>500	0.009116577768176001	3.18904713343927
>1k	0.003038859256058667	2.3181919546923924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTCACGTTGGGGGATGCTGAAACTGAAACTCCAGTACTTTGGCCACCTGAT	3909	0.25888805955816174	No Hit
GACCACGTTGGGGAGGTACTGGAGGGAGAGACGTGGGCACAAAGGTCGCGGG	2196	0.1454382652314462	No Hit
TACCACGTTGGGGGGAATTGTGAAGTGATGAGCTAAACTGGGTGAGTTGGAG	1772	0.11735728870224163	No Hit
AATCACGTTGGGGCGGGAGTATGATGAGGCGTGCCATCCTGCAGGAAGCTGC	1673	0.11080064559754531	No Hit
CTTTACGTTGGGGGATGGCAAAAATTCACCACCTCTTTGACCCGTGAAATAT	1533	0.10152862504544946	No Hit
GGCGACGTTGGGGTTAAGTACAACTACGACTGCCCCCTTGAGACAAGGCCTG	1529	0.10126371017253244	No Hit
GCTTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1528	0.10119748145430318	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.008212361060427744	0.0	0.0	0.0	0.0
12	0.05324788945632183	0.0	0.0	0.0	0.0
13	0.2464370605310616	0.0	0.0	0.0	0.0
14	0.6745394951649725	0.0	0.0	0.0	0.0
15	1.233244962146976	0.0	0.0	0.0	0.0
16	1.5678324466411775	0.0	0.0	0.0	0.0
17	1.770293638268013	0.0	0.0	0.0	0.0
18	2.042228755317338	0.0	0.0	0.0	0.0
19	2.272241093727544	0.0	0.0	0.0	0.0
20	2.498279709043995	0.0	0.0	0.0	0.0
21	2.707761144803132	0.0	0.0	0.0	0.0
22	2.9513503704503354	0.0	0.0	0.0	0.0
23	3.20553619101422	0.0	0.0	0.0	0.0
24	3.4925052271015864	0.0	0.0	0.0	0.0
25	3.8006674530223146	0.0	0.0	0.0	0.0
26	4.131546129295677	0.0	0.0	0.0	0.0
27	4.455801933746115	0.0	0.0	0.0	0.0
28	4.785952094118956	0.0	0.0	0.0	0.0
29	5.1335866361043205	0.0	0.0	0.0	0.0
30	5.510825415138163	0.0	0.0	0.0	0.0
31	5.847797133488617	0.0	0.0	0.0	0.0
32	6.140263153189012	0.0	0.0	0.0	0.0
33	6.411337296901357	0.0	0.0	0.0	0.0
34	6.657045841531897	0.0	0.0	0.0	0.0
35	6.894277110229091	0.0	0.0	0.0	0.0
36	7.1142226834684505	0.0	0.0	0.0	0.0
37	7.3359564321	0.0	0.0	0.0	0.0
38	7.552987941737272	0.0	0.0	0.0	0.0
39	7.77750329653445	0.0	0.0	0.0	0.0
40	8.000429162094125	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCT	155	0.0	46.000004	19
TATTTAG	35	1.0198528E-7	46.000004	14
CGTGGTT	70	0.0	46.000004	42
TCCGCCG	70	0.0	46.000004	20
TGCCAAT	35	1.0198528E-7	46.000004	35
CATTGTT	70	0.0	46.000004	46
ACCGCCA	35	1.0198528E-7	46.000004	35
GACCGTC	70	0.0	46.000004	28
ACACAAT	70	0.0	46.000004	36
CTAAGTT	155	0.0	46.000004	24
TGTGCTT	35	1.0198528E-7	46.000004	14
CCTAAGT	155	0.0	46.000004	23
CCAGACC	70	0.0	46.000004	25
TTTCACA	140	0.0	46.000004	38
ATACTCA	35	1.0198528E-7	46.000004	17
ATTTTTC	140	0.0	46.000004	35
CTTATAA	140	0.0	46.000004	15
TCTAACT	35	1.0198528E-7	46.000004	28
GATATTT	140	0.0	46.000004	32
ACAGCTT	70	0.0	46.000004	39
>>END_MODULE