FastQCFastQC Report
Fri 17 Jun 2016
SRR1528057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378719
Sequences flagged as poor quality0
Sequence length59
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCACGTTGGGGGATGCTGAAACTGAAACTCCAGTACTTTGGCCACCTGATGCAAAGA31730.23014116727193865No Hit
GACCACGTTGGGGAGGTACTGGAGGGAGAGACGTGGGCACAAAGGTCGCGGGAGGAACA19220.1394047663084356No Hit
TACCACGTTGGGGGGAATTGTGAAGTGATGAGCTAAACTGGGTGAGTTGGAGATTCTAA13930.10103581658046346No Hit
GGCGACGTTGGGGTTAAGTACAACTACGACTGCCCCCTTGAGACAAGGCCTGAGCTCTG13890.10074569219688712No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGTA1100.053.00000429
TTAGGAT550.053.00000445
CTGGTCA550.053.00000438
TCACTCC1100.053.00000422
TTACACC550.053.00000431
CCTGAGC1550.053.00000449
TACACCT550.053.00000432
TTCCTTA550.053.00000427
CTTACAC550.053.00000430
ACAACTG800.053.025
GTGCATT401.6007107E-953.023
GTCATGT401.6007107E-953.025
CATGGCT503.6379788E-1253.040
TTAGGCT503.6379788E-1253.028
CTGTCTG401.6007107E-953.023
GATACTC503.6379788E-1253.016
ACTATAC950.053.045
TCTGACC401.6007107E-953.052
TACCCGT401.6007107E-953.039
GATGTTT203.1384645E-453.032