##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528057_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1378719
Sequences flagged as poor quality	0
Sequence length	59
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.482713301260084	31.0	31.0	31.0	28.0	34.0
2	31.25397053351698	31.0	31.0	34.0	30.0	34.0
3	31.531739970218734	31.0	31.0	34.0	30.0	34.0
4	35.30392269925924	35.0	35.0	37.0	33.0	37.0
5	35.01673002257893	35.0	35.0	37.0	32.0	37.0
6	35.443109146969036	37.0	35.0	37.0	33.0	37.0
7	35.10940590504664	37.0	35.0	37.0	32.0	37.0
8	33.72747673746427	35.0	33.0	36.0	30.0	37.0
9	36.70132347490678	37.0	35.0	39.0	33.0	39.0
10	36.350678419605444	38.0	35.0	39.0	32.0	39.0
11	37.14950762265552	39.0	37.0	39.0	34.0	39.0
12	37.140555834800274	39.0	37.0	39.0	35.0	39.0
13	36.8919765376411	39.0	37.0	39.0	32.0	39.0
14	36.27999106416899	39.0	36.0	40.0	29.0	40.0
15	34.764881748927806	37.0	33.0	40.0	25.0	40.0
16	35.07359150051606	38.0	33.0	40.0	26.0	40.0
17	34.9587450379664	38.0	33.0	40.0	25.0	40.0
18	35.45532773538335	38.0	34.0	40.0	27.0	40.0
19	35.857713573251694	38.0	34.0	40.0	30.0	40.0
20	35.90809874963644	38.0	34.0	40.0	30.0	40.0
21	35.9283276722813	38.0	34.0	40.0	30.0	40.0
22	35.84301659729067	38.0	34.0	40.0	30.0	40.0
23	35.737903082499045	38.0	34.0	40.0	29.0	40.0
24	35.738795214978545	38.0	34.0	40.0	29.0	40.0
25	35.55600307241722	38.0	34.0	40.0	27.0	40.0
26	34.96477962514479	38.0	33.0	39.0	25.0	40.0
27	34.18507977332582	37.0	33.0	39.0	24.0	40.0
28	33.64737339515884	35.0	33.0	38.0	24.0	40.0
29	33.404449347546525	35.0	32.0	38.0	23.0	40.0
30	33.14561777998272	35.0	31.0	38.0	22.0	40.0
31	32.855015416484434	35.0	31.0	38.0	21.0	40.0
32	32.49888991157734	35.0	31.0	38.0	21.0	40.0
33	32.1239643466145	35.0	31.0	38.0	20.0	40.0
34	31.88566343105448	35.0	31.0	38.0	18.0	40.0
35	31.735530590352347	35.0	30.0	38.0	17.0	40.0
36	31.18518929528062	35.0	29.0	38.0	15.0	40.0
37	30.955696556005975	35.0	28.0	38.0	15.0	40.0
38	30.466209575700343	35.0	26.0	37.0	12.0	40.0
39	29.542709573161755	34.0	24.0	37.0	10.0	40.0
40	28.701524386042404	33.0	22.0	36.0	9.0	40.0
41	27.69003618576374	33.0	18.0	35.0	8.0	39.0
42	26.78543923743707	33.0	16.0	35.0	8.0	39.0
43	25.925139930616755	33.0	12.0	35.0	8.0	39.0
44	25.188675865060247	31.0	10.0	35.0	8.0	38.0
45	24.820204842321026	31.0	10.0	35.0	8.0	38.0
46	24.33816970680755	30.0	10.0	35.0	8.0	38.0
47	23.79292299591142	28.0	9.0	35.0	8.0	37.0
48	23.366236339674728	27.0	9.0	35.0	8.0	36.0
49	22.985232668875963	27.0	9.0	35.0	8.0	36.0
50	22.451662013796867	24.0	9.0	34.0	8.0	35.0
51	22.036653589310077	23.0	9.0	34.0	8.0	35.0
52	21.433104207601403	22.0	8.0	34.0	8.0	35.0
53	21.150792148363806	21.0	8.0	33.0	8.0	35.0
54	20.791078530142837	20.0	8.0	33.0	8.0	35.0
55	20.404204917753365	20.0	8.0	33.0	7.0	35.0
56	19.84207369304405	18.0	8.0	33.0	7.0	35.0
57	19.223475559559272	15.0	8.0	33.0	6.0	35.0
58	18.66004965478825	13.0	8.0	33.0	6.0	35.0
59	17.87329325264974	10.0	8.0	31.0	6.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	15.0
14	65.0
15	271.0
16	922.0
17	2626.0
18	5717.0
19	10749.0
20	17890.0
21	26732.0
22	37616.0
23	50626.0
24	64933.0
25	80480.0
26	96142.0
27	104644.0
28	91933.0
29	70699.0
30	64367.0
31	69940.0
32	77828.0
33	87651.0
34	99585.0
35	103072.0
36	105207.0
37	74474.0
38	32594.0
39	1937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.26213898553657	21.278737726831938	38.65080556661655	3.808317721014942
2	22.000494662073997	22.944341812943755	35.45247436207088	19.602689162911368
3	19.443991125095106	23.348485079265608	37.350177955043776	19.857345840595507
4	15.725321838605256	20.25343815527312	36.22406016019218	27.79717984592945
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.40531754476439	0.3208775682354417	0.18212558179005295	0.09167930521012621
12	98.7230175256887	0.7274868918176945	0.1993154515169516	0.35018013097665296
13	92.80890449758073	4.775012167091336	1.255150614447179	1.1609327208807596
14	39.16613900294404	39.55534086351171	11.036331551244308	10.242188582299946
15	24.471193912610183	56.605007982047105	12.882465535036507	6.041332570306205
16	31.759118428048065	48.71101362931823	12.403470177751958	7.126397764881749
17	16.371936558501044	51.83746651783285	18.408827324494695	13.381769599171406
18	16.01007892108544	54.963556750868015	17.720652286651596	11.305712041394948
19	20.63038226063469	52.390516124025275	19.03890495452663	7.940196660813408
20	15.659826259012895	55.15968083416563	19.803382705250307	9.377110201571169
21	18.926481755890794	52.55886079759545	14.272596518942585	14.242060927571174
22	19.752248282644977	59.03044782874538	12.475130900495316	8.742172988114328
23	14.253230716338864	55.45306911705721	14.587961723890075	15.705738442713852
24	18.62489745916318	58.43424222049598	10.846445142193586	12.094415178147251
25	18.848220703421074	50.98624157641986	18.080333991190372	12.085203728968702
26	18.86004327205181	52.38667197594289	18.109273898452113	10.64401085355319
27	21.551672240681384	55.0276017085425	14.86452279253423	8.55620325824189
28	23.453727699407928	55.14727801676774	13.665511246309073	7.733483037515258
29	26.33234183325246	53.858255380538026	10.661345785471877	9.14805700073764
30	27.47158775646089	55.90769402612135	9.211231585261391	7.409486632156372
31	20.01974296430237	60.747839117325576	11.356411277424915	7.87600664094714
32	17.752420906653203	64.65733771711277	10.460942367516514	7.1292990087175125
33	16.047287373279108	67.2059353646392	8.691111096604892	8.055666165476794
34	13.428116969447727	69.37367222762579	9.012061195936226	8.186149606990258
35	15.592662464214971	67.63386883041433	9.261132979236523	7.512335726134187
36	11.99250898841606	67.01728198421868	12.655225611600333	8.334983415764924
37	13.269926649302722	67.8352151526163	10.915857400964228	7.979000797116743
38	14.66382925019529	65.71527628182392	11.656109765659282	7.964784702321502
39	14.365653914974697	64.2509459868182	12.117842722121042	9.265557376086063
40	17.235999503887303	63.41074577198109	10.621598744921917	8.731655979209686
41	17.98655128419932	61.98993413451181	9.964031829546121	10.05948275174274
42	19.62510127154264	58.70543598804397	9.815923331730396	11.853539408682987
43	21.109377617919243	57.49880867674994	11.211349085636739	10.180464619694078
44	20.559446848850275	55.15641693485039	13.576443060551135	10.707693155748197
45	21.083846672164526	54.675173113593125	12.836916006815022	11.404064207427329
46	22.79325954019637	55.064810160736165	11.197423115225074	10.944507183842394
47	22.77106502485278	54.462004222760406	11.935354484851517	10.831576267535299
48	22.390494364696504	54.48702745084386	10.919121300279462	12.20335688418017
49	21.44345584560741	54.5159673581056	11.112489201933098	12.92808759435389
50	20.92319029475912	54.90930349113924	11.883277157999563	12.284229056102077
51	20.915574529690243	54.99626827511624	11.592935181135534	12.495222014057978
52	20.903534367771822	53.36765504791041	12.623166867215147	13.105643717102614
53	19.913920095392896	52.89526002035223	13.141691671761976	14.049128212492901
54	19.93328589799662	51.92319827317967	13.497819352601944	14.645696476221767
55	19.577883528115592	50.187964335009525	14.452038450184556	15.782113686690327
56	20.079218462935522	47.94319944818342	14.99050930610226	16.987072782778796
57	20.47226447158558	45.44406800805675	16.179366498902244	17.904301021455424
58	20.915647060786135	43.212213656299795	17.1084898373055	18.763649445608568
59	21.898588472342805	39.66362978968158	19.688275856066394	18.74950588190922
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	1.5
5	3.0
6	6.5
7	10.0
8	290.5
9	1899.5
10	3228.0
11	7681.0
12	12134.0
13	15321.0
14	17849.0
15	17190.0
16	13737.0
17	10284.0
18	9423.0
19	10523.5
20	12485.0
21	14458.5
22	16432.0
23	20431.5
24	24431.0
25	31840.5
26	51194.5
27	63139.0
28	73045.5
29	82952.0
30	91205.5
31	102954.5
32	106450.0
33	105382.0
34	104314.0
35	99706.5
36	89831.5
37	84564.0
38	78043.5
39	71523.0
40	68835.5
41	66148.0
42	62543.5
43	56969.5
44	55000.0
45	54342.0
46	53684.0
47	51272.5
48	46855.0
49	44849.0
50	42280.0
51	39711.0
52	39252.0
53	36290.0
54	33787.0
55	29794.5
56	25802.0
57	21574.5
58	14387.0
59	11427.0
60	8575.5
61	5724.0
62	5018.5
63	4313.0
64	3400.0
65	1517.5
66	548.0
67	413.0
68	278.0
69	218.5
70	124.0
71	89.0
72	75.0
73	61.0
74	44.5
75	37.0
76	46.0
77	36.5
78	27.0
79	18.5
80	10.0
81	7.5
82	3.0
83	1.0
84	2.0
85	3.0
86	2.0
87	1.0
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1378719.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.84650076307878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.0807276577416	65.71209616137456
2	2.831423921295075	3.9553010615867197
3	0.8435510944460083	1.7675727648575719
4	0.4384396031386486	1.2249388830075032
5	0.25844471766791133	0.9025729584902725
6	0.19002234445747768	0.7963437496290742
7	0.1526450066298786	0.7463203700437787
8	0.11928784186256769	0.6665470670143885
9	0.10317541704817453	0.6485797661027665
>10	0.898411301701333	12.396385132179603
>50	0.042158842362568574	1.9398379675494883
>100	0.03554056731934204	5.619315594819384
>500	0.0046819674222653125	2.2023989195144305
>1k	0.0014897169070844176	1.4217896038305624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTCACGTTGGGGGATGCTGAAACTGAAACTCCAGTACTTTGGCCACCTGATGCAAAGA	3173	0.23014116727193865	No Hit
GACCACGTTGGGGAGGTACTGGAGGGAGAGACGTGGGCACAAAGGTCGCGGGAGGAACA	1922	0.1394047663084356	No Hit
TACCACGTTGGGGGGAATTGTGAAGTGATGAGCTAAACTGGGTGAGTTGGAGATTCTAA	1393	0.10103581658046346	No Hit
GGCGACGTTGGGGTTAAGTACAACTACGACTGCCCCCTTGAGACAAGGCCTGAGCTCTG	1389	0.10074569219688712	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.006962985205832371	0.0	0.0	0.0	0.0
12	0.04576712150916902	0.0	0.0	0.0	0.0
13	0.2202769382303428	0.0	0.0	0.0	0.0
14	0.5975836990713844	0.0	0.0	0.0	0.0
15	1.0810034532054755	0.0	0.0	0.0	0.0
16	1.3738840184257997	0.0	0.0	0.0	0.0
17	1.5454925913112099	0.0	0.0	0.0	0.0
18	1.7724423903638087	0.0	0.0	0.0	0.0
19	1.9732809948945362	0.0	0.0	0.0	0.0
20	2.1667214276440667	0.0	0.0	0.0	0.0
21	2.3414488376529228	0.0	0.0	0.0	0.0
22	2.5483075231428596	0.0	0.0	0.0	0.0
23	2.764232595619557	0.0	0.0	0.0	0.0
24	3.00634139371402	0.0	0.0	0.0	0.0
25	3.260127698247431	0.0	0.0	0.0	0.0
26	3.53233690113794	0.0	0.0	0.0	0.0
27	3.8043285107407674	0.0	0.0	0.0	0.0
28	4.074071656370878	0.0	0.0	0.0	0.0
29	4.365284006385638	0.0	0.0	0.0	0.0
30	4.672018010921732	0.0	0.0	0.0	0.0
31	4.955687126963507	0.0	0.0	0.0	0.0
32	5.198883891496382	0.0	0.0	0.0	0.0
33	5.425978752740769	0.0	0.0	0.0	0.0
34	5.635448557682893	0.0	0.0	0.0	0.0
35	5.83432882262448	0.0	0.0	0.0	0.0
36	6.025085604825929	0.0	0.0	0.0	0.0
37	6.215189607164332	0.0	0.0	0.0	0.0
38	6.399636184022995	0.0	0.0	0.0	0.0
39	6.5835025121145065	0.0	0.0	0.0	0.0
40	6.772591079110391	0.0	0.0	0.0	0.0
41	6.958995995558196	0.0	0.0	0.0	0.0
42	7.1533793325543495	0.0	0.0	0.0	0.0
43	7.35320250174256	0.0	0.0	0.0	0.0
44	7.558828158602297	0.0	0.0	0.0	0.0
45	7.761117385050906	0.0	0.0	0.0	0.0
46	7.9570964061567295	0.0	0.0	0.0	0.0
47	8.141252858631818	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGTA	110	0.0	53.000004	29
TTAGGAT	55	0.0	53.000004	45
CTGGTCA	55	0.0	53.000004	38
TCACTCC	110	0.0	53.000004	22
TTACACC	55	0.0	53.000004	31
CCTGAGC	155	0.0	53.000004	49
TACACCT	55	0.0	53.000004	32
TTCCTTA	55	0.0	53.000004	27
CTTACAC	55	0.0	53.000004	30
ACAACTG	80	0.0	53.0	25
GTGCATT	40	1.6007107E-9	53.0	23
GTCATGT	40	1.6007107E-9	53.0	25
CATGGCT	50	3.6379788E-12	53.0	40
TTAGGCT	50	3.6379788E-12	53.0	28
CTGTCTG	40	1.6007107E-9	53.0	23
GATACTC	50	3.6379788E-12	53.0	16
ACTATAC	95	0.0	53.0	45
TCTGACC	40	1.6007107E-9	53.0	52
TACCCGT	40	1.6007107E-9	53.0	39
GATGTTT	20	3.1384645E-4	53.0	32
>>END_MODULE