FastQCFastQC Report
Fri 17 Jun 2016
SRR1528056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270216
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGTGGTAGGGACCACCCACAGAGAAGATCCAACTCACCTGCCTCTTCCA37480.29506792545519817No Hit
CCTCGTGGTAGGGGTCAACCATTTGCTCTCCCAAGCCACTGGGCCATGGCCT33290.2620814097759751No Hit
TAAAGTGGTAGGGGGTTGTGGGGACGGTCACAGATGATGTGCGGATTCTGGA31490.24791059158442344No Hit
GGCTGTGGTAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCC30720.24184863046914856No Hit
TAGGGTGGTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTG28210.22208821176870705No Hit
CGTGGTGGTAGGGGAACTGCCGGGCGCAGTGGCTCATGCCTGGAATCCCAGC26910.2118537319636975No Hit
ACTGGTGGTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACT26740.21051537691227318No Hit
CACAGTGGTAGGGGCCAGGCAGGATACCAGGCCCAAGGATGTTCAGAATAGT26680.21004301630588815No Hit
CGGGGTGGTAGGGTGCTTAAAAGTTAACATCTCCGGCCGGGCGCGGTGGCTC26170.20602795115161515No Hit
TACCGTGGTAGGGAGGCCCTGCTGTTGGCATCCTGCTATCTGCCCCCTGCCT25610.20161925215868795No Hit
GCGAGTGGTAGGGAGTGAAGGAGCAGGACTGTTACAGCCTCTGCCAAACCAG25430.20020217033953283No Hit
ATCTGTGGTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG23610.18587389861251946No Hit
GATCGTGGTAGGGTTTGGCGGGCTGAGGTGGGAGAATCACTTAGCCCAGGAA23570.18555899154159608No Hit
GCTCGTGGTAGGGGGACTTCCCCGGGACCATGCTGCGCTGGCTGCGGGCCTT20570.16194096122234328No Hit
CGGAGTGGTAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCC20350.16020897233226475No Hit
CCGGGTGGTAGGGTTTGGGAGGCCGAGGCGGGCAGATCACCTGAGGTTAGGA20150.1586344369776479No Hit
ACATGTGGTAGGGCTTTTTCCAGCCTCCGCCTCGGACCCTGCAGCCGCCGCG20100.1582408031389937No Hit
GCTGGTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT19050.1499744925272552No Hit
GAAGGTGGTAGGGACCGTGTTACTGCATGCGCCCCTGAAGCTCTGTGTTCTT18640.14674669505029067No Hit
CTATGTGGTAGGGGTCTCTGCTTTCTACATGCTGAGAGTAAAAGTACACACC18490.14556579353432803No Hit
GGTGGTGGTAGGGGTTCTGCCTGTAGGGAAGCTGGAGCTCACAGGCAAAATA18140.14281035666374853No Hit
TAAGGTGGTAGGGGTTCCCTGGCTCCACCCCCCACGCCTCTTAAGTACCAAG18090.14241672282509432No Hit
CTTGGTGGTAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG17980.14155072838005503No Hit
GGGGGTGGTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACT17830.1403698268640924No Hit
GCCTGTGGTAGGGGAGCTGAGATGAAGTAACAAGGCTGAAGGAAGGAATTCC17390.13690584908393533No Hit
TTGCGTGGTAGGGAACTATGTTCTGTTTCTTTGCTAAAGCTCTGTGTTTTCC17300.13619730817435774No Hit
CAGAGTGGTAGGGATCTCCGCAGTTGGGCAAAATGACTTCTGCCGAGCTGGA17260.13588240110343439No Hit
TCTAGTGGTAGGGTCAACCAGAGTTTATGTGGTAGGGGCCGGTAGTGGTGGC17220.135567494032511No Hit
TCTCGTGGTAGGGGAGGCCACAGTCTGCGGTGTCTGCGGGGGTGGCTGACTG17070.13438659251654836No Hit
TTGCGTGGTAGGGATCACTATTAGTTCTTCTGGCGCCTGCCAGAAGTAACAG17040.13415041221335583No Hit
TTGTGTGGTAGGGGGAACCCCCCAACGCACAATACTGGGCCCAGTTCCACAG16770.1320247894846231No Hit
GGTTGTGGTAGGGGACAACAACAGATCAAGCGAACTGCCCGGACTTCCTGTA16700.13147370211050718No Hit
GTTGGTGGTAGGGGGGTTCGGGTGTGGGAAGCGGGAGCCCTGGCTCCCGCCG16680.1313162485750455No Hit
ATAGGTGGTAGGGGGTGTTCGCAGCCTTCGCCGCGCCGCTGCCGCTCTCCAA16420.1292693526140436No Hit
GGTAGTGGTAGGGGGGAGTGTGGCGGAGCCCGCAGCTTCAGCAGCTCCGCGG16000.1259628283693482No Hit
GAGGGTGGTAGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATG15930.1254117409952323No Hit
TAATGTGGTAGGGCACTGTGACTTGATGGGAGACTGTTATCTGAGTCCACAG15850.12478192685338556No Hit
CCGTGTGGTAGGGGCTCTTCGGTTCTCATCGCTGTGCGGACGCCAGAGCCGA15820.12454574655019303No Hit
TGTGGTGGTAGGGGGACATACATGGTATGTACTCAATGATAAGTGGATATTA15820.12454574655019303No Hit
ACTCGTGGTAGGGATGTGGATCCTAACCAACGCGCAAACATCACAAAGGGAA15740.12391593240834631No Hit
ACATGTGGTAGGGGTCGTGACGCCACGCTTCTGGCCTGTCGCCTCCGTCTGC15710.12367975210515376No Hit
TACGGTGGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG15700.12360102533742293No Hit
TTCGGTGGTAGGGCGGGTGGTGGTGGTGCACGCCTTTAATCCCAGCACTCGG15630.12304993796330703No Hit
TTAAGTGGTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG15500.12202648998280607No Hit
GTGGGTGGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTG15440.12155412937642102No Hit
AGCTGTGGTAAGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG15440.12155412937642102No Hit
ACATGTGGTAGGGGTTTCCTCAGTGTGTATGCCCAGCAGTGGGATTGCTGGA15430.12147540260869016No Hit
GGCCGTGGTAGGGGGAGCTAGAGAGATGGCTCAGTGGTTAAGAGCACTGACT15380.12108176877003596No Hit
ACTAGTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTGGGAGCACCCGAC15370.12100304200230512No Hit
GTTCGTGGTAGGGCGGTGGTAGGGGGAGTCCCAAGAGACAGGAAGAGGAAGA15350.12084558846684344No Hit
AGGGGTGGTAGGGGTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAA15310.12053068139592006No Hit
CTTTGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACT15280.12029450109272753No Hit
AGGTGTGGTAGGGGGGCCCCCGTGCCAAGCAAAGACCAAAGATGCATCAGCT15240.11997959402180416No Hit
TGGCGTGGTAGGGCGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGC15120.11903487280903405No Hit
GGGTGTGGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGAC15010.11816887836399478No Hit
GACTGTGGTAGGGCTTTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGA14910.11738161068668634No Hit
GCCGGTGGTAGGGGAGAATTCGGTCCCGGAGCAACCTGGCGAGACGGCTCAA14840.11683052331257046No Hit
CTATGTGGTAGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAG14730.1159645288675312No Hit
GGGTGTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT14720.11588580209980034No Hit
TGGAGTGGTAGGGAGGTGGGGGAGAGAGGAGGTTCTGAGAAAGTATTTGCTG14700.11572834856433867No Hit
ATTTGTGGTAGGGGAACCCTGCCCCTCACTCCTATTTATTAAGCATCACACT14690.11564962179660783No Hit
TGCCGTGGTAGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGA14610.11501980765476108No Hit
AATGGTGGTAGGGACAAGCAGCTGCGCAACCCTAGCCTCGTCCTGTTGTGAC14560.11462617381610687No Hit
GTTGGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACT14520.1143112667451835No Hit
GGGCGTGGTAGGGGAGGTCGGCGAGTGCTCTGCGGTGGCCGGTGTCTGCAGG14490.11407508644199096No Hit
GGACGTGGTAGGGGAAGTCCAGGCCTTACTTTCGTACACAATGCCCTATCCC14380.11320909199695169No Hit
TTTTGTGGTAGGGGGAACACGGCTGTGCTCCCTCCTCGTCGCCCTGTACTGC14380.11320909199695169No Hit
CTTTGTGGTAGGGACAGCCGAACACTTAACGCCGCGCCGGAGCCACCGCACC14330.11281545815829748No Hit
TACTGTGGTAGGGTACCCTCCCGGGTGCTGCTTCTCCTTCCTGCCCACCCTA14250.11218564401645076No Hit
TGGTGTGGTAGGGGACTTCCGGCGTGGCCATGGCGGCTAACGCGACCACGAA14130.11124092280368064No Hit
GTATGTGGTAGGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAA14070.11076856219729557No Hit
CTTGGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACT14060.11068983542956473No Hit
CGCGGTGGTAGGGACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTC13960.1099025677522563No Hit
GGTTGTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC13960.1099025677522563No Hit
CTAAGTGGTAGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGAT13950.10982384098452547No Hit
GGCCGTGGTAGGGGATTTCAAAATTTAGTTACTTCTTTTTTTTTGAGATGGA13880.10927275361040957No Hit
AGGTGTGGTAGGGATCTGGAGACCGTGGTACAGCCAAGACCCAAGCACCTCA13880.10927275361040957No Hit
CCTCGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACT13630.1073045844171385No Hit
CCCAGTGGTAGGGCTCCTCCTCCTCTGTGGTGGTTGGGGCAGCTGAGTGAGA13570.10683222381075344No Hit
GGCTGTGGTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC13500.10628113643663754No Hit
TCCAGTGGTAGGGACCAAGGTTGATGCACCCAGCCTGAAAGGACTGATTAAT13460.10596622936571419No Hit
GCTTGTGGTAGGGGGGGCTGGAGAGATGGCTCAGCTGGTAAGAGCACTGACT13410.10557259552705996No Hit
CCTTGTGGTAGGGACCGGGCGGAGCGGCCAACATGGCGGAACGCAGGCGACA13410.10557259552705996No Hit
GACAGTGGTAGGGAGGCAATCCTCCCTAACTCATTCTGTGAAACCAGTATGC13400.10549386875932912No Hit
AAAAGTGGTAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGAC13400.10549386875932912No Hit
TCCTGTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT13330.10494278138521324No Hit
GGTCGTGGTAGGGGGCCGGTAGTGGTGGCGCGCGCCGGTAGGATTTGCTGAA13330.10494278138521324No Hit
ACGAGTGGTAGGGCAGGATCTTCTGGAAACCCAAGATCCAGGGGTTCTGAAG13230.1041555137079048No Hit
AGTCGTGGTAGGGGATTGAGCTCCATAGAGACAGCGCCGGGGCAAGCGCGAG13210.10399806017244312No Hit
GCCTGTGGTAGGGAACGCTTTCTTCAAAGGTGCTTGGTCCAATGTACTGAGA13120.10328951926286553No Hit
TCGTGTGGTAGGGATCTTATTGACCCAGATATATTTTGATCAACGGACCAAG13080.10297461219194216No Hit
AATGGTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGGCACCGACT13020.10250225158555709No Hit
TACTGTGGTAGGGTGCTACCTCACTCCCTGCCTGGTGCTCAAACTCATCCTT12930.10179371067597952No Hit
CAAGGTGGTAGGGTTTGGCTGGTGAGATGGCTCAGCGGATAGGAGCACTTGA12840.10108516976640193No Hit
TTGGGTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT12820.10092771623094025No Hit
ATATGTGGTAGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTA12750.10037662885682434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTGT351.0196891E-746.00000420
CGGAACC351.0196891E-746.00000415
GTACCTT351.0196891E-746.00000430
TTGGCGC700.046.00000427
CCGGGTT351.0196891E-746.00000418
CCCCTCC351.0196891E-746.00000439
ATTAGAT700.046.00000414
AATCAAT351.0196891E-746.00000435
AATCAAG700.046.00000414
GTACACT700.046.00000433
AAATACG351.0196891E-746.00000418
CCCTATG700.046.00000442
ACTGACC351.0196891E-746.00000446
ACGTTCG700.046.00000442
GGCACGG700.046.00000432
CGGCCCC351.0196891E-746.00000429
GCGCGAT700.046.00000421
TCTTCAC351.0196891E-746.00000431
ACCGCGA351.0196891E-746.00000431
ATAATAT351.0196891E-746.00000437
Produced by FastQC (version 0.11.5)